Rhizopogon roseolus (Corda) Th. Fr.’ un kültürel özellikleri
Yükleniyor...
Tarih
2017
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada Rhizopogon roseolus (Corda) Th. Fr.’un spor ve miselyumlarının anatomik ve morfolojik özellikleri çalışıldı. Mantar örnekleri Kırıkkale-Türkiye’den toplandı ve laboratuvara getirildi. Alınan doku örnekleri patates dekstroz agar (PDA) besiyerinde doku kültürü yöntemi ile gelişti. 25C’de inkübe edildi. Miseller inokulasyondan 2 gün sonra besiyeri üzerinde karışık olarak gelişim gösterdi. Özellikle koloninin en dış bölgesindeki misellerde yoğunluğun arttığı ve pigmentasyon oluşumu gözlendi. Misellerde önce hafif sarımsı renkte gelişme olduğu daha sonra koloninin en dışında pembemsi pigmentasyon geliştiği görüldü. Kolonizasyon oluşumu 20 günde tamamlandı R.roseolus’un spor ve miselleri hem ışık hem de scanning elektron mikroskop (SEM) ile incelendi. R.roseolus’un sporları düz ve boyutları 3.5-4x6.5-7 µm. olup genellikle oval şekillidir.
In this study, the morphological and anatomical features of Rhizopogon roseolus (Corda) Th. Fr. spores and mycelium were examined. The mushroom samples were collected from Kırıkkale-Turkey and brought to the laboratory. Taken tissue fragments were grown on potato dextrose agar (PDA) medium by tissue culture method. They were incubated in 25C. After 2 days from inoculation mycelium showed mixed growth on the agar medium. Particularly, in the outermost regions of the colony, density increased and pigmentation was observed. It was first observed to develop a slightly yellowish color and then pinkish pigmentation developed at the outermost part of the colony. Colonization occurred in 20 days. The spores and mycelium of R.roseolus were examined by both light microscopy and scanning electron microscopy (SEM). The spores of R. roseolus are smooth and have dimensions of .5-4x6.5-7 µm. usually oval shape.
In this study, the morphological and anatomical features of Rhizopogon roseolus (Corda) Th. Fr. spores and mycelium were examined. The mushroom samples were collected from Kırıkkale-Turkey and brought to the laboratory. Taken tissue fragments were grown on potato dextrose agar (PDA) medium by tissue culture method. They were incubated in 25C. After 2 days from inoculation mycelium showed mixed growth on the agar medium. Particularly, in the outermost regions of the colony, density increased and pigmentation was observed. It was first observed to develop a slightly yellowish color and then pinkish pigmentation developed at the outermost part of the colony. Colonization occurred in 20 days. The spores and mycelium of R.roseolus were examined by both light microscopy and scanning electron microscopy (SEM). The spores of R. roseolus are smooth and have dimensions of .5-4x6.5-7 µm. usually oval shape.
Açıklama
Anahtar Kelimeler
Biyoloji, Mikroskopi
Kaynak
Biological Diversity and Conservation
WoS Q Değeri
Scopus Q Değeri
Cilt
10
Sayı
3
Künye
Güler, P. (2017). Rhizopogon roseolus (Corda) Th. Fr.’ un kültürel özellikleri. Biological Diversity and Conservation, 10(3), 79 - 84.
