High frequency shoot regeneration from immature embryo explants of Hungarian vetch

dc.contributor.authorSancak, Cengiz
dc.contributor.authorMirici, Semra
dc.contributor.authorÖzcan, Sebahattin
dc.date.accessioned2020-06-25T15:13:11Z
dc.date.available2020-06-25T15:13:11Z
dc.date.issued2000
dc.departmentKırıkkale Üniversitesi
dc.description.abstractEfficient and reproducible shoot regeneration has been established from immature cotyledons and embryo axes of Hungarian vetch. Immature embryo axes showed higher regeneration capacity than immature cotyledons in most media tested. Immature cotyledons appeared to produce the highest frequency of shoot regeneration on 20 ?M 6-benzylaminopurine (BA) and 2.5 ?M ?-naphthaleneacetic acid (NAA)-containing medium; whereas, shoot regeneration on immature embryo axes was best achieved on a medium supplemented with 5 ?M each of BA and NAA. Regenerated shoots were excised and rooted in half-strength MS medium supplemented with 5 ?M indole-3-butyric acid (IBA). Rooted plantlets were acclimatized to ambient conditions and later established under greenhouse conditions.en_US
dc.description.sponsorshipThe authors wish to thank the State Planning Organization of Turkey and Ankara University for financial support.en_US
dc.identifier.doi10.1023/A:1006426013350
dc.identifier.endpage235en_US
dc.identifier.issn01676857
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-0033784319
dc.identifier.scopusqualityQ1
dc.identifier.startpage231en_US
dc.identifier.urihttps://doi.org/10.1023/A:1006426013350
dc.identifier.urihttps://hdl.handle.net/20.500.12587/1641
dc.identifier.volume61en_US
dc.indekslendigikaynakScopus
dc.language.isoen
dc.relation.ispartofPlant Cell, Tissue and Organ Culture
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectImmature cotyledonen_US
dc.subjectImmature embryo axisen_US
dc.subjectOrganogenesisen_US
dc.subjectVicia pannonicaen_US
dc.titleHigh frequency shoot regeneration from immature embryo explants of Hungarian vetchen_US
dc.typeArticle

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