Role of nicotinic acetylcholine receptor subtypes on nicotine's enhancing effect on electrical field stimulation elicited contractile responses in rabbit urine bladder

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dc.contributor.authorFincan, G. S. Ozturk
dc.contributor.authorVural, I. M.
dc.contributor.authorYildirim, S. S.
dc.contributor.authorIsli, F.
dc.contributor.authorDilekoz, E.
dc.contributor.authorErcan, S.
dc.contributor.authorSarioglu, Y.
dc.date.accessioned2020-06-25T18:16:40Z
dc.date.available2020-06-25T18:16:40Z
dc.date.issued2016
dc.departmentKırıkkale Üniversitesi
dc.descriptionSARIOGLU, YUSUF/0000-0002-9227-365X
dc.description.abstractOBJECTIVE: This study aims to investigate the contribution of presynaptic nicotinic acetylcholine receptors (nAChRs) sub-types to nicotine-induced enhancement in electrical field stimulation (EFS) EFS-mediated contractile responses in rabbit urine bladder smooth muscle preparations. MATERIALS AND METHODS: Rabbit urine bladder smooth muscle strips were placed in organ baths containing 20 ml of an aerated Krebs-Henseleit solution, and contractions were recorded using isometric force displacement transducers. Following the acquisition of control EFS (60 V, 8 Hz, 1 ms) responses, nicotine was added to the bath at a 3x10(-5) M concentration, and EFS responses were obtained. The effect of nAChR antagonists on nicotine-induced augmentation in EFS-mediated responses was investigated in the presence of hexamethonium, dihydro-beta-erythroidine, mecamy-lamine, and alpha-bungarotoxin. RESULTS: Tetrodotoxin (TTX; 10(-6) M) completely blocked EFS-induced contractile responses in smooth muscle strips. Similarly, Atropine (10(-6) M), when administered with alpha,beta-methylene adenosine triphosphate (alpha,beta-methylene-ATP) (10(-5) M), completely blocked EFS responses. Nicotine significantly enhanced EFS-mediated contractile responses (23.67% +/- 1.75). Nicotine-induced increases in EFS responses were largely inhibited by hexamethonium, mecamylamine, and dihydro-beta-erythroidine, whereas alpha-bungarotoxin only partly inhibited these enhancements. CONCLUSIONS: These findings demonstrate that EFS-induced neurogenic contractions in rabbit urine bladder smooth muscle strips are mediated by purinergic and cholinergic transmissions, and the alpha 4 beta 2, alpha 3 beta 4, and alpha 7 sub-types of nAChRs contribute to the enhancement effect of nicotine on EFS-induced contractile responses.en_US
dc.description.sponsorshipGazi University Unit of Scientific Research ProjectsGazi University [01/2006-17]en_US
dc.description.sponsorshipThis work was supported by the Gazi University Unit of Scientific Research Projects (Project number: 01/2006-17).en_US
dc.identifier.citationOzturk Fincan, G. S., Vural, I. M., Yildirim, S. S., Isli, F., Dilekoz, E., Ercan, S., & Sarioglu, Y. (2016). Role of nicotinic acetylcholine receptor subtypes on nicotine's enhancing effect on electrical field stimulation elicited contractile responses in rabbit urine bladder. European review for medical and pharmacological sciences, 20(8), 1636–1641.en_US
dc.identifier.endpage1641en_US
dc.identifier.issn1128-3602
dc.identifier.issue8en_US
dc.identifier.pmid27160140
dc.identifier.startpage1636en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12587/6591
dc.identifier.volume20en_US
dc.identifier.wosWOS:000380260000030
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherVerduci Publisheren_US
dc.relation.ispartofEuropean Review For Medical And Pharmacological Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectNicotineen_US
dc.subjectElectrical field stimulationen_US
dc.subjectNicotinic acetylcholine receptorsen_US
dc.subjectUrine bladderen_US
dc.titleRole of nicotinic acetylcholine receptor subtypes on nicotine's enhancing effect on electrical field stimulation elicited contractile responses in rabbit urine bladderen_US
dc.typeArticle

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