Studies of adsorption of alkaline trypsin by poly(methacrylic acid) brushes on chitosan membranes

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dc.contributor.authorBayramoglu, Guelay
dc.contributor.authorErdogan, Hakan
dc.contributor.authorArica, M. Yakup
dc.date.accessioned2020-06-25T17:44:45Z
dc.date.available2020-06-25T17:44:45Z
dc.date.issued2008
dc.description.abstractPoly(methacrylic acid)-grafted chitosan membranes (chito an-g-poly(MAA)) were prepared in two sequential steps: in the first step, chitosan membranes were prepared by phase-inversion technique and then epichlorohydrin was used as crosslinking agent to increase its chemical stability in acidic media; in the second step, the graft copolymerization of methacrylic acid onto the chitosan membranes was initiated by ammonium persulfate (APS) under nitrogen atmosphere. The chitosan-g-poly(MAA) membranes were first used as an ion-exchange support for adsorption of trypsin from aqueous solution. The influence of pH, equilibrium time, ionic strength, and initial trypsin concentration on the adsorption capacity of the chitosan-g-poly(MAA) membranes have been investigated in a batch system. Maximum trypsin adsorption onto chitosan-gpoly(MAA) membrane was found to be 92.86 mg mL(-1) at pH 7.0. The experimental equilibrium data obtained for trypsin adsorption onto chitosan-g-poly(MAA) membranes fitted well to the Langmuir isotherm model. The adsorption data was analyzed using the first- and second-order kinetic models, and the experimental data was well described by the second-order equation. More than 97% of the adsorbed trypsin was desorbed using glutamic acid solution (0.5M, pH 4.0). In addition, the chitosan-gpoly(MAA) membrane prepared in this work showed promising potential for various biotechnological applications. (C) 2007 Wiley Periodicals, Inc.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1002/app.27690
dc.identifier.endpage465en_US
dc.identifier.issn0021-8995
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-40549091336
dc.identifier.scopusqualityQ2
dc.identifier.startpage456en_US
dc.identifier.urihttps://doi.org10.1002/app.27690
dc.identifier.urihttps://hdl.handle.net/20.500.12587/4184
dc.identifier.volume108en_US
dc.identifier.wosWOS:000253287600059
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherJohn Wiley & Sons Incen_US
dc.relation.ispartofJournal Of Applied Polymer Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectchitosan-g-poly(MAA) membraneen_US
dc.subjection-exchange membraneen_US
dc.subjectpolymer brushesen_US
dc.subjectadsorptionen_US
dc.subjecttrypsinen_US
dc.subjectthermodynamic parametersen_US
dc.titleStudies of adsorption of alkaline trypsin by poly(methacrylic acid) brushes on chitosan membranesen_US
dc.typeArticle

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