Activity of 3?-hydroxysteroid dehydrogenase associated with progesterone production in bovine granulosa cells cultured under diferent concentrations of serum, insulin-like growth factor I, and gonadotropin
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Tarih
2014
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info:eu-repo/semantics/openAccess
Özet
Three-&#946;-hydroxysteroid dehydrogenase (3&#946;-HSD) is the enzyme responsible for progesterone production. Tis study aimed to determine whether 3&#946;-HSD activity can be shown to refect progesterone production by granulosa cells cultured under diferent serum conditions and follicle stimulating hormone (FSH), luteinising hormone (LH), and insulin-like growth factor I (IGF-I) concentrations. Large bovine follicles were dissected from abattoir ovaries to recover granulosa cells. Cells were washed, stained for viability, and plated for 48 h in basic medium with or without 5% foetal calf serum (FCS). Subsequently, cells were exposed to FSH (1 ng/mL), LH (10 or 100 ng/mL), or FSH (1 ng/mL) + IGF-I (1 or 10 ng/mL) in a serum-free medium for another 96 h to predict degree of luteinisation. Before and afer incubation, granulosa cells were stained for 3&#946;-HSD activity. Te high dose of IGF-I (10 ng/mL) increased (P < 0.05) progesterone secretion over 2.5-fold compared with FSH alone or the low dose of IGF-I (1 ng/mL) in cells preincubated with FCS. Tis was clearly refected by darker 3&#946;-HSD staining than in cells exposed to FSH and low dose IGF-I.
Açıklama
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Kaynak
Turkish Journal of Veterinary and Animal Sciences
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Scopus Q Değeri
Cilt
38
Sayı
4
