Activity of 3?-hydroxysteroid dehydrogenase associated with progesterone production in bovine granulosa cells cultured under diferent concentrations of serum, insulin-like growth factor I, and gonadotropin
| dc.contributor.author | Şimşek, Özkan | |
| dc.contributor.author | Mıhm, Monika | |
| dc.date.accessioned | 2021-01-14T18:16:17Z | |
| dc.date.available | 2021-01-14T18:16:17Z | |
| dc.date.issued | 2014 | |
| dc.description.abstract | Three-&#946;-hydroxysteroid dehydrogenase (3&#946;-HSD) is the enzyme responsible for progesterone production. Tis study aimed to determine whether 3&#946;-HSD activity can be shown to refect progesterone production by granulosa cells cultured under diferent serum conditions and follicle stimulating hormone (FSH), luteinising hormone (LH), and insulin-like growth factor I (IGF-I) concentrations. Large bovine follicles were dissected from abattoir ovaries to recover granulosa cells. Cells were washed, stained for viability, and plated for 48 h in basic medium with or without 5% foetal calf serum (FCS). Subsequently, cells were exposed to FSH (1 ng/mL), LH (10 or 100 ng/mL), or FSH (1 ng/mL) + IGF-I (1 or 10 ng/mL) in a serum-free medium for another 96 h to predict degree of luteinisation. Before and afer incubation, granulosa cells were stained for 3&#946;-HSD activity. Te high dose of IGF-I (10 ng/mL) increased (P < 0.05) progesterone secretion over 2.5-fold compared with FSH alone or the low dose of IGF-I (1 ng/mL) in cells preincubated with FCS. Tis was clearly refected by darker 3&#946;-HSD staining than in cells exposed to FSH and low dose IGF-I. | en_US |
| dc.identifier.endpage | 362 | en_US |
| dc.identifier.issn | 1300-0128 | |
| dc.identifier.issn | 1303-6181 | |
| dc.identifier.issue | 4 | en_US |
| dc.identifier.startpage | 358 | en_US |
| dc.identifier.trdizinid | 165898 | |
| dc.identifier.uri | https://app.trdizin.gov.tr/makale/TVRZMU9EazRPQT09 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12587/13088 | |
| dc.identifier.volume | 38 | en_US |
| dc.indekslendigikaynak | TR-Dizin | |
| dc.language.iso | en | |
| dc.relation.ispartof | Turkish Journal of Veterinary and Animal Sciences | |
| dc.relation.publicationcategory | Makale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.subject | Ziraat Mühendisliği | en_US |
| dc.title | Activity of 3?-hydroxysteroid dehydrogenase associated with progesterone production in bovine granulosa cells cultured under diferent concentrations of serum, insulin-like growth factor I, and gonadotropin | en_US |
| dc.type | Article |
