Investigation of the relationship between betatrophin and certain key enzymes involved in carbohydrate and lipid metabolism in insulin-resistant mice

Basit Öğe Kaydı
dc.authoridYASEMIN, USTUNDAG/0000-0002-8836-0371
dc.authoridDINDAR BADEM, NERMIN/0000-0002-5095-7818
dc.contributor.authorArikan, Funda Bulut
dc.contributor.authorUlas, Mustafa
dc.contributor.authorUstundag, Yasemin
dc.contributor.authorBoyunaga, Hakan
dc.contributor.authorBadem, Nermin Dindar
dc.date.accessioned2025-01-21T16:42:13Z
dc.date.available2025-01-21T16:42:13Z
dc.date.issued2023
dc.departmentKırıkkale Üniversitesi
dc.description.abstractObjectives The present study sought to examine the relationship of betatrophin with certain key enzymes, namely lactate dehydrogenase-5 (LDH5), citrate synthase (CS), and acetyl-CoA carboxylase-1 (ACC1), in insulin-resistant mice.Methods Eight-week-old male C57BL6/J mice were used in this study (experimental group n=10 and control group n=10). S961 was administered using an osmotic pump to induce insulin resistance in the mice. The betatrophin, LDH5, CS, and ACC1 expression levels were determined from the livers of the mice using the real-time polymerase chain reaction (RT-PCR) method. Moreover, biochemical parameters such as the serum betatrophin, fasting glucose, insulin, triglyceride, total cholesterol, and high-density lipoprotein (HDL) and low-density lipoprotein (LDL) cholesterol levels were analyzed.Results The betatrophin expression and serum betatrophin (p=0.000), fasting glucose, insulin, triglyceride (p <= 0.001), and total cholesterol (p=0.013) levels were increased in the experimental group. In addition, the CS gene expression level was statistically significantly decreased in the experimental group (p=0.01). Although strong correlation was found between the expression and serum betatrophin and triglyceride levels, no correlation was found between the betatrophin gene expression and the LDH5, ACC1, and CS gene expression levels.Conclusions The betatrophin level appears to play an important role in the regulation of triglyceride metabolism, while insulin resistance increases both the betatrophin gene expression and serum levels and decreases the CS expression level. The findings suggest that betatrophin may not regulate carbohydrate metabolism through CS and LDH5 or lipid metabolism directly through the ACC1 enzyme.
dc.description.sponsorshipScientific Research Projects Coordination Unit of Kirikkale University [2015/43]
dc.description.sponsorshipThis study was supported by Scientific Research Projects Coordination Unit of Kirikkale University (project number: 2015/43)
dc.identifier.doi10.1515/hmbci-2022-0104
dc.identifier.endpage320
dc.identifier.issn1868-1883
dc.identifier.issn1868-1891
dc.identifier.issue3
dc.identifier.pmid36869875
dc.identifier.startpage311
dc.identifier.urihttps://doi.org/10.1515/hmbci-2022-0104
dc.identifier.urihttps://hdl.handle.net/20.500.12587/25019
dc.identifier.volume44
dc.identifier.wosWOS:000943608100001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWalter De Gruyter Gmbh
dc.relation.ispartofHormone Molecular Biology and Clinical Investigation
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectacetyl-coa carboxylase; betatrophin; carbohydrate and lipid metabolism; citrate synthase; lactate dehydrogenase
dc.titleInvestigation of the relationship between betatrophin and certain key enzymes involved in carbohydrate and lipid metabolism in insulin-resistant mice
dc.typeArticle

Dosyalar

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu