Evaluation of immunohistochemical expression of GSTA1 and GSTP1 isoenzymes before and after treatment of Trx and L-NAME in experimental hepatic ischemia/reperfusion model

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dc.contributor.authorGüler Şimşek G.
dc.contributor.authorKiliç M.
dc.contributor.authorOğuz?üzun S.
dc.contributor.authorKarahan S.
dc.contributor.authorAkin O.K.
dc.contributor.authorKiliç N.
dc.contributor.authorSerdar M.A.
dc.date.accessioned2020-06-25T15:17:18Z
dc.date.available2020-06-25T15:17:18Z
dc.date.issued2012
dc.departmentKırıkkale Üniversitesi
dc.description.abstractIschemia/reperfusion (I/R) causes formation of Reactive Oxygen Species (ROS) in tissues, in response to which injured cells improve a number of defense mechanisms including Glutathione S-Transferases (GSTs). The aim of this study was to investigate the expressions of GSTA1 and GSTP1 following Thioredoxin (Trx) and N-nitro-L-arginine methyl ester (L-NAME) treatment in a rat model of hepatic I/R model. A total of 50 Wistar rats were randomly allocated into 5 groups: sham (n = 10), control (I/R) (n = 10), Trx (n = 10), L-NAME (n = 10), and Trx+L-NAME (n = 10). With an exception to those in sham group, all rats were subjected to a hepatic ischemia process for an hour and then subsequent reperfusion. GSTA1 and GSTP1 expressions in the liver tissues were determined by immunohistochemical method. The GSTA1 expression was absent in sham group while varying degrees of expression occurred in other groups. The GSTA1 expression was significantly higher in Trx/L-NAME group compared to other groups (p <0.05). GSTP1 expression was no difference between groups (p >0.05). As a result, we think that GSTA1 expression may have increased in response to I/R as a part of the liver oxygen radical scavenging process.en_US
dc.identifier.endpage30en_US
dc.identifier.issn13004182
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84904566446
dc.identifier.scopusqualityQ4
dc.identifier.startpage23en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2281
dc.identifier.volume37en_US
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSociety of Pharmaceutical Sciences of Ankara (FABAD)en_US
dc.relation.ispartofFabad Journal of Pharmaceutical Sciences
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGSTA1 and GSTP1en_US
dc.subjectHepatic ischemia/reperfusionen_US
dc.titleEvaluation of immunohistochemical expression of GSTA1 and GSTP1 isoenzymes before and after treatment of Trx and L-NAME in experimental hepatic ischemia/reperfusion modelen_US
dc.typeArticle

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