Synthesis and characterization of polyethyleneimine/silk fibroin/gold nanoparticle nanocomposites: Potential application as a gene carrier in breast cancer cell lines

Basit Öğe Kaydı
dc.contributor.authorTunali, Beste Cagdas
dc.contributor.authorAkturk, Omer
dc.contributor.authorSahingoz, Durukan
dc.contributor.authorTurk, Mustafa
dc.contributor.authorKeskin, Ayten Celebi
dc.date.accessioned2025-01-21T16:44:48Z
dc.date.available2025-01-21T16:44:48Z
dc.date.issued2023
dc.departmentKırıkkale Üniversitesi
dc.description.abstractThere is a need in advancing the design of non-viral gene carriers, thus herein using the pulsed laser ablation in liquid (PLAL) technique, polyethyleneimine-capped gold nanoparticles (PEI-AuNPs) were synthesized and then combined with Bombyx mori silk fibroin (SF) to form the nanocomposites (PEI-SF-AuNPs). After the characterization analyses were carried out, PEI-AuNPs and PEI-SF-AuNPs were tested for their in vitro biocompatibility in MCF-7 and MDA-MB231 breast cancer cells. Finally, the transfection capability of the selected group was observed. PEI capping enabled the cationic modification of the AuNP surface and SF reduced the surface charge (from 35 to 4 mV). PEI-AuNPs were highly monodispersed in shape and size (8-9 nm). The nanoparticle group synthesized in 5% (w/v) PEI concentration (PEI-AuNP5) had increased cytotoxicity as compared to the group synthesized in 1% (w/v) PEI concentration (PEI-AuNP1), but the conjugation of SF with PEI-AuNPs alleviated this cytotoxic tendency significantly. The group (PEI-AuNP1) with the most negligible cytotoxicity proved to be successful in the transfection of siRNA analog, i.e., siGLO green transfection indicator. Overall, the PEI-AuNP1 nanocomposites could be suggested as potent siRNA carriers due to the above beneficial properties in genebased breast cancer therapies.
dc.description.sponsorshipScientific ResearchProjects Coordination Unit of Kirikkale University [2017/033]
dc.description.sponsorshipAcknowledgment This project was financially supported by The Scientific ResearchProjects Coordination Unit of Kirikkale University (No: 2017/033)
dc.identifier.doi10.1016/j.eurpolymj.2023.112042
dc.identifier.issn0014-3057
dc.identifier.issn1873-1945
dc.identifier.scopus2-s2.0-85152115024
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.eurpolymj.2023.112042
dc.identifier.urihttps://hdl.handle.net/20.500.12587/25512
dc.identifier.volume191
dc.identifier.wosWOS:000983439600001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherPergamon-Elsevier Science Ltd
dc.relation.ispartofEuropean Polymer Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectPolyethyleneimine; Silk fibroin; Gold nanoparticle; Gene carrier; siRNA; Transfection
dc.titleSynthesis and characterization of polyethyleneimine/silk fibroin/gold nanoparticle nanocomposites: Potential application as a gene carrier in breast cancer cell lines
dc.typeArticle

Dosyalar

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu