The comparison of changes in fecal and mucosal microbiome in metabolic endotoxemia induced by a high-fat diet

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dc.contributor.authorSagkan-Ozturk, Aliye
dc.contributor.authorArpaci, Abdullah
dc.date.accessioned2025-01-21T16:28:36Z
dc.date.available2025-01-21T16:28:36Z
dc.date.issued2022
dc.departmentKırıkkale Üniversitesi
dc.description.abstractThe aim of this study was to compare the mucosal and fecal microbiota in a high fat diet-induced metabolic endotoxemia (ME) model and to identify potential species that represent dysbiosis and might mediate the inflammatory process. Fourteen male wistar albino rats were fed a standard diet (n = 7) and a high-fat diet (HFD) (n = 7). The standard diet (2600 kcal/kg) contained 3% of energy from fat and HFD (6740 kcal/kg) contained 67% beef tallow. After feeding for 12 weeks, all rats were sacrificed after fasting for 12 h and blood samples were collected. Fresh faecal samples and descending colon samples of rats were collected in sterile plastic tubes using a clean technique, immediately snap-frozen in liquid nitrogen, and then stored at ?80 °C until used for analysis. Serum glucose, TRG, TLR4, LPS, and fecal LPS increased in the HFD group. On the contrary, HDL was higher and statistically significant in the CD group. The levels of IL-6 and TNF-? in the colon tissue of the HFD group were significant. The HFD group caused a significant increase in LPS levels in serum and feces. In addition, the gut and mucosal microbiome were positively/negatively correlated with the ME markers (IL-6, TNF-?, LPS). The results showed that gut and mucosal microbiome changes were associated with HFD. These changes were dense at species levels. The current study demonstrated changes in gut and mucosal microbiota in HFD-induced metabolic endotoxemia. © 2022 Elsevier Ltd
dc.identifier.doi10.1016/j.anaerobe.2022.102615
dc.identifier.issn1075-9964
dc.identifier.pmid35850456
dc.identifier.scopus2-s2.0-85135702282
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.anaerobe.2022.102615
dc.identifier.urihttps://hdl.handle.net/20.500.12587/23592
dc.identifier.volume77
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAcademic Press
dc.relation.ispartofAnaerobe
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectFeces; High fat diet; Metabolic endotoxemia; Microbiota; Mucosa
dc.titleThe comparison of changes in fecal and mucosal microbiome in metabolic endotoxemia induced by a high-fat diet
dc.typeArticle

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