Molecular Analysis of Three Local Silkworm Breeds (Alaca, Bursa Beyazi and Hatay Sarisi) by RAPD-PCR and SDS-PAGE Methods

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dc.authoridBUDAK YILDIRAN, Fatma Azize/0000-0001-7031-6834
dc.contributor.authorAkkir, Demet Eroglu
dc.contributor.authorYildiran, F. Azize Budak
dc.contributor.authorCakir, Suekran
dc.date.accessioned2025-01-21T16:42:45Z
dc.date.available2025-01-21T16:42:45Z
dc.date.issued2010
dc.departmentKırıkkale Üniversitesi
dc.description.abstractIn this study, PCR-based RAPD method was used to determine the genetic variation in three Turkish local silkworm breeds (Alaca, Bursa Beyazi and Hatay Sarisi) and SDS-PAGE method was used to analyse the sericin proteins of their cocoons. Three local breeds were analysed by randomly chosen 40 primers and 68 total RAPD bands observed with 7 of them. Percentages of polymorphic loci were determined higher in Hatay Sarisi (55.88), lower in Bursa Beyazi and Alaca (44.12). Nei's genetic distance was determined 0.0637 between Bursa Beyazi and Alaca, 0.1012 between Bursa Beyazi and Hatay Sarisi, 0.0793 between Alaca and Hatay Sarisi. In SDS-PAGE sericin proteins of three breeds was yielded a single band of 200 kDa.
dc.identifier.doi10.9775/kvfd.2010.2022
dc.identifier.endpageS269
dc.identifier.issn1300-6045
dc.identifier.issn1309-2251
dc.identifier.scopusqualityQ3
dc.identifier.startpageS265
dc.identifier.urihttps://doi.org/10.9775/kvfd.2010.2022
dc.identifier.urihttps://hdl.handle.net/20.500.12587/25124
dc.identifier.volume16
dc.identifier.wosWOS:000281800400015
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherKafkas Univ, Veteriner Fakultesi Dergisi
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241229
dc.subjectBombyx mori; RAPD-PCR; Genetic polymorphism; SDS-PAGE
dc.titleMolecular Analysis of Three Local Silkworm Breeds (Alaca, Bursa Beyazi and Hatay Sarisi) by RAPD-PCR and SDS-PAGE Methods
dc.typeArticle

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