Effects of cholesterol, FSH and LH on steroidogenic activity of cat granulosa cells cultured in vitro
Yükleniyor...
Tarih
2015
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Brazilian Coll Animal Reproduction
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The aim of this study was to examine the effects of 22R-hydroxycholesterol (22R-HC), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) on estradiol and progesterone production by cat granulosa cells. Granulosa cells from follicles were collected and cultured for up to 5 days in 24 well plates containing Dulbecco's Modified Eagle's Medium (DMEM)/HAM F-12 supplemented with 10(-7) M androstenedione, 0.1% ITS premix and 0.1% bovine serum albumin, in the presence or absence of 22R-HC (10 mu g/ml), FSH or LH (10, 100 ng/ml each) on first and third day. Additionally, 5% fetal calf serum was added into the culture medium for the first 24 h. Treatment of cells with 22R-HC resulted in an increase (P < 0.05) in progesterone and estradiol production on days 3 and 5 of the culture. Incubation of cells with FSH (10 and 100 ng/ml) resulted in significant stimulations of progesterone (P < 0.001) whilst incubation had no effect on estradiol production. None of the LH doses (10 and 100 ng/ml) had any effect on progesterone production by granulosa cells during the culture time. With the inclusion of 22R-HC into the culture system, progesterone synthesis was enhanced (P < 0.001) in the presence of all FSH doses.
Açıklama
Anahtar Kelimeler
cat, estradiol, FSH, granulosa cell culture, LH, progesterone
Kaynak
Animal Reproduction
WoS Q DeÄŸeri
Q3
Scopus Q DeÄŸeri
Cilt
12
Sayı
4
Künye
closedAccess
