Effects of cholesterol, FSH and LH on steroidogenic activity of cat granulosa cells cultured in vitro

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dc.contributor.authorSimsek, O.
dc.contributor.authorArikan, S.
dc.date.accessioned2020-06-25T18:12:46Z
dc.date.available2020-06-25T18:12:46Z
dc.date.issued2015
dc.departmentKırıkkale Üniversitesi
dc.description.abstractThe aim of this study was to examine the effects of 22R-hydroxycholesterol (22R-HC), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) on estradiol and progesterone production by cat granulosa cells. Granulosa cells from follicles were collected and cultured for up to 5 days in 24 well plates containing Dulbecco's Modified Eagle's Medium (DMEM)/HAM F-12 supplemented with 10(-7) M androstenedione, 0.1% ITS premix and 0.1% bovine serum albumin, in the presence or absence of 22R-HC (10 mu g/ml), FSH or LH (10, 100 ng/ml each) on first and third day. Additionally, 5% fetal calf serum was added into the culture medium for the first 24 h. Treatment of cells with 22R-HC resulted in an increase (P < 0.05) in progesterone and estradiol production on days 3 and 5 of the culture. Incubation of cells with FSH (10 and 100 ng/ml) resulted in significant stimulations of progesterone (P < 0.001) whilst incubation had no effect on estradiol production. None of the LH doses (10 and 100 ng/ml) had any effect on progesterone production by granulosa cells during the culture time. With the inclusion of 22R-HC into the culture system, progesterone synthesis was enhanced (P < 0.001) in the presence of all FSH doses.en_US
dc.description.sponsorshipKirikkale University Research FundKirikkale University [2010/03]en_US
dc.description.sponsorshipFinancial support for this project was provided by the Kirikkale University Research Fund (Project No. 2010/03). The authors would like to thank Prof. Dr. Hakan Kalender for his assistance in collecting the ovaries from the cats.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.endpage938en_US
dc.identifier.issn1806-9614
dc.identifier.issn1984-3143
dc.identifier.issue4en_US
dc.identifier.startpage931en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12587/6037
dc.identifier.volume12en_US
dc.identifier.wosWOS:000365735900010
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherBrazilian Coll Animal Reproductionen_US
dc.relation.ispartofAnimal Reproduction
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcaten_US
dc.subjectestradiolen_US
dc.subjectFSHen_US
dc.subjectgranulosa cell cultureen_US
dc.subjectLHen_US
dc.subjectprogesteroneen_US
dc.titleEffects of cholesterol, FSH and LH on steroidogenic activity of cat granulosa cells cultured in vitroen_US
dc.typeArticle

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