A Comparison of the Effect of Methyl-β-Cyclodextrin on the Osmotic Fragility of Ovine, Bovine and Human Erythrocytes
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Tarih
2003
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Siklodekstrinler gıda, kozmetik, ilaç ve kimya sanayiinde kullanım alanı bulan bazı etken maddelerin çözünürlüğünü ve stabilitesini artırmak, etken maddelerde bulunan bazı istenmeyen tat ve kokuları maskelemek için yaygın olarak kullanılmaktadır. Bu çalışmada pre-hemolitik dozda kullanılan metil-ß-siklodekstrinin (MBCD) koyun, inek ve insan eritrositi ozmotik frajilitesi üzerine etkisi araştırıldı.Bu üç türden elde edilen heparinli kan, alımı takiben üç saat içinde analiz edildi. Eritrositlerden 2, 4 veya 6 mM MBCD içerecek şekilde süspansiyonlar hazırlandı. Karışım 37 \circC ısıda 30 dk inkübe edildikten sonra santrifüj edildi. Tekrar sulandırılan eritrositlerin ozmotik frajiliteleri ölçüldü. Her üç türde de eritrosit süspansiyonu ile inkübe edilen MBCD, eritrositlerin ozmotik frajilitesinde doza paralel olarak artan bir ozmotik frajiliteye yol açtı. Hemoliz başlangıcı koyun, inek ve insan kontrol gruplarında sırası ile %0,85, %0,70 ve %0,55 NaCl olarak bulundu. Bunun aksine MBCD uygulanan grupların hepsinde de hemoliz, bundan çok daha erken başladı. Eritrositlerin ozmotik frajilitesi NaCl solüsyonunun %0.70 (P0.01), %0.75 (P0.001) ve %0.80 (P0.001) konsantrasyonlarında koyunda, %0.55 (P0.01), %0.60 (P0.001) ve %0.65 (P0.01) konsantrasyonlarında inekte ve %0.40 (P0.01), %0.45 (P0.001) ve %0.50 (P0.01) konsantrasyonlarında ise insanda kontrol gruplarınkinden daha fazla bulundu. Sonuç olarak; pre-hemolitik dozda kullanılan siklodextrine bağlı olarak eritrosit frajilitesinde meydana gelen artış, bu makromolekülün düşük dozlarının dahi membran komponentlerini uzaklaştırarak, eritrosit membran dayanıklılığını azalttığına işaret etmektedir.
Cyclodextrins are used extensively in food, cosmetic, drug and chemical industries to increase aqueous solubility and stability, and reduce or eliminate the unpleasant taste and smell of many products. Experiments were conducted to examine the effect of a pre-haemolytic dose of methyl ß-cyclodextrin (MBCD) on the osmotic fragility of erythrocytes collected from sheep, cattle and humans.Heparinised blood collected from these three species were analysed within 3 h of collection. Erythrocyte suspensions were mixed with MBCD to give concentrations of 2 mM, 4 mM or 6 mM cyclodextrin. The mixtures were incubated for 30 min at 37 \circC and centrifuged. The osmotic fragility of re-suspended erythrocytes was measured.The incubation of MBCD with the erythrocyte suspensions induced a dose-dependent increase in the osmotic fragility of erythrocytes obtained from all three species. The beginning of haemolysis in control groups of sheep, cattle and humans occurred at 0.85%, 0.70% and 0.55% NaCl, respectively. However, haemolysis in the MBCD-treated groups began earlier than this in all the three species studied. The osmotic fragility of erythrocytes was significantly higher at NaCl concentrations of 0.70% (P > 0.01), 0.75% (P > 0.001) and 0.80% (P > 0.001) in sheep; 0.55% (P > 0.01), 0.60% (P > 0.001) and 0.65% (P > 0.01) in cattle; and 0.40% (P > 0.01), 0.45% (P > 0.001) and 0.50% (P > 0.01) in humans than in the controls. In conclusion, these results indicate that a pre-haemolytic dose of MBCD may also induce membrane disruption which elicits removal of membrane components from erythrocytes.
Cyclodextrins are used extensively in food, cosmetic, drug and chemical industries to increase aqueous solubility and stability, and reduce or eliminate the unpleasant taste and smell of many products. Experiments were conducted to examine the effect of a pre-haemolytic dose of methyl ß-cyclodextrin (MBCD) on the osmotic fragility of erythrocytes collected from sheep, cattle and humans.Heparinised blood collected from these three species were analysed within 3 h of collection. Erythrocyte suspensions were mixed with MBCD to give concentrations of 2 mM, 4 mM or 6 mM cyclodextrin. The mixtures were incubated for 30 min at 37 \circC and centrifuged. The osmotic fragility of re-suspended erythrocytes was measured.The incubation of MBCD with the erythrocyte suspensions induced a dose-dependent increase in the osmotic fragility of erythrocytes obtained from all three species. The beginning of haemolysis in control groups of sheep, cattle and humans occurred at 0.85%, 0.70% and 0.55% NaCl, respectively. However, haemolysis in the MBCD-treated groups began earlier than this in all the three species studied. The osmotic fragility of erythrocytes was significantly higher at NaCl concentrations of 0.70% (P > 0.01), 0.75% (P > 0.001) and 0.80% (P > 0.001) in sheep; 0.55% (P > 0.01), 0.60% (P > 0.001) and 0.65% (P > 0.01) in cattle; and 0.40% (P > 0.01), 0.45% (P > 0.001) and 0.50% (P > 0.01) in humans than in the controls. In conclusion, these results indicate that a pre-haemolytic dose of MBCD may also induce membrane disruption which elicits removal of membrane components from erythrocytes.
Açıklama
Anahtar Kelimeler
Ziraat Mühendisliği
Kaynak
Turkish Journal of Veterinary and Animal Sciences
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
27
Sayı
2
Künye
Arıkan, Ş. (2003). A comparison of the effect of methyl-$\beta$ -cyclodextrin on the osmotic fragility of ovine, bovine and human erythrocytes. Turkish Journal of Veterinary and Animal Sciences, 27(2), 383 - 387.
