Novel hydrophobic ligand-containing hydrogel membrane matrix: Preparation and application to ?-globulins adsorption

Simple item page
dc.contributor.authorArica M.Y.
dc.contributor.authorAkin-Öktem G.
dc.contributor.authorDenizli A.
dc.date.accessioned2020-06-25T15:13:14Z
dc.date.available2020-06-25T15:13:14Z
dc.date.issued2001
dc.departmentKırıkkale Üniversitesi
dc.description.abstractIn this study, phenylalanine as a hydrophobic ligand was covalently attached to the co-monomer methacrylochloride. Then, poly(2-hydroxyethylmethacrylate-co-methacrylamidophenyalanine) [poly(HEMA-MAPA)] membranes were prepared by UV-initiated photopolymerization of HEMA and methacrylamidophenyalanine. The ?-globulins adsorption onto these affinity membranes from aqueous solutions containing different amounts of ?-globulins at different pH was investigated in a batch system. The ?-globulins adsorption capacity of the membranes was increased as the ligand density on the membrane surface increase. The non-specific adsorption of the ?-globulins on the pHEMA membranes was negligible. The adsorption phenomena appeared to follow a typical Langmuir isotherm. The maximum adsorption capacity (qm) of the poly(HEMA-MAPA4) membrane for ?-globulins was 2.37 mg g-1dry membrane. The equilibrium constant (kd) value was found to be 1.61×10-1mg ml-1. More than 87% (up to 100%) of the adsorbed ?-globulins were desorbed in 120 min in the desorption medium containing 50% ethylene glycol in 1.0 M NaCl. Copyright © 2001 Elsevier Science B.V.en_US
dc.identifier.doi10.1016/S0927-7765(00)00209-5
dc.identifier.endpage283en_US
dc.identifier.issn09277765
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-0035002089
dc.identifier.scopusqualityQ1
dc.identifier.startpage273en_US
dc.identifier.urihttps://doi.org/10.1016/S0927-7765(00)00209-5
dc.identifier.urihttps://hdl.handle.net/20.500.12587/1678
dc.identifier.volume21en_US
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevieren_US
dc.relation.ispartofColloids and Surfaces B: Biointerfaces
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAffinity membraneen_US
dc.subjectHydrophobic liganden_US
dc.subjectPhenylalanineen_US
dc.subjectProtein separationen_US
dc.subject?-globulinsen_US
dc.titleNovel hydrophobic ligand-containing hydrogel membrane matrix: Preparation and application to ?-globulins adsorptionen_US
dc.typeArticle

Files

Collections

Scopus İndeksli Yayınlar Koleksiyonu