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Öğe Absence of estrogen and progesterone receptors around the affected vessels of angioma serpiginosum - Case report(Adis Int Ltd, 2006) Erkek, Emel; Bozdoğan, Önder; Akarsu, Cengiz; Atasoy, Pınar; Koçak, MukadderAngioma serpiginosum is a disorder of dilated superficial dermal capillaries. The disease is considered by some authors to be a consequence of increasing levels of estrogens. We present a case of angioma serpiginosum in a 46-year-old White woman. The lesions consisting of red-purple puncta were grouped as vaguely annular and angular patches with serpiginous borders, located on the right side of the neck, the right side of the abdomen, and on the left arm. Histopathologic examination revealed clusters of dilated capillaries in the dermal papillae, without endothelial proliferation. Periodic acid-Schiff (PAS) stain-positive deposits were noted around the affected blood vessels. However, immunohistochemical analysis revealed the absence of estrogen and progesterone receptors within the involved blood vessels. Hormonal assays were also normal. Our observations suggest that a hormonal stimulus probably plays no role in the pathogenesis of angioma serpiginosum. The significance of PAS-positive deposits as a diagnostic marker for angioma serpiginosum needs further confirmation.Öğe Absence of human papillomavirus antigens in cutaneous soft fibromas(2003) Koçak, Mukadder; Erkek, Emel; Bozdoğan, Önder; Birol, Ahu; Atasoy, PınarGiriş: Derinin yumuşak fibromlarının gerçek nedeni bilinmemekle birlikte deri yaşlanması, şişmanlık, diyabet, gebelik, menopoz, akromegali ve genetik yatkınlık ile ilişkili olabilecekleri ileri sürülmektedir. Son zamanlarda insan papilloma virüsleri de yumuşak fibrom oluşumunda potansiyel etyolojik faktör olarak suçlanmışlardır. Amaç: Bu çalışmada yumuşak fibrom etyopatogenezinde insan papilloma virüslerinin rolünün araştırılması amaçlanmış ve bu lezyonların diyabet ve obezite ile ilşkileri de sorgulanmıştır. Hastalar ve Yöntem: Bu amaçla 37 hastanın lezyonel deri örneklerinde immünhistokimyasal metod ile HPV antijenleri çalışılmıştır. Her hastadan açlık ve tokluk kan şekeri ve gayitada gizli kan tetkikleri rutin olarak istenmiştir.Bulgular: Lezyonel deri biyopsilerinden yalnızca birinde (2.7 %) HPV antijen varlığı saptanmıştır. On-üç hastanın (35.1 %) vücut kütle indeksi 30'un üstünde hesaplanmıştır. Açlık ve/ veya tokluk kan şekeri 14 hastada (37.8 %) yüksek bulunmuştur. Sonuç: Bu bulgular çok sayıda yumuşak fibrom ile başvuran hastalarda karbonhidrat metabolizmasının değerlendirilmesi gerektiğine işaret etmekte ve insan papilloma virüslerinin bu lezyonların etyolojisinde rolü olmadığını düşündürmektedir.Öğe Ailesel urtikarya pigmentoza(2000) Erkek, Emel; Bozdoğan, ÖnderUrtikarya pigmentoza, dermiste mast hücresi infiltrasyonu ile karakterize, nadir görülen bir deri hastalığıdır. Genelde sporadik olarak görülmektedir, ancak ailesel olgular da bildirilmektedir. Burada bir anne ve kızında eş zamanda görülen bir urtikarya pigmentoza olgusu sunulmakta, hastalığın olası genetik geçişi tartışılmaktadır.Öğe Analysis of estrogen and progesterone receptors in lesional and normal skin of patients with acne rosacea(2003) Erkek, Emel; Bozdoğan, Önder; Koçak, Mukadder; Birol, Ahu; Atasoy, PınarGiriş: Rozase etyolojisinde hormonal faktörlerin rol oynayabileceğine ilişkin çok sayıda kanıt bulunmaktadır. Lezyonel deride östrojen reseptörlerinin azalması ve progesteron reseptörlerinin artışı hipotetik olarak rozase oluşumunda önemli olabilir. Amaç: Bu çalışmada rozaseli hastaların lezyonel ve normal deri dokularında östrojen ve progesteron reseptörlerinin ekspresyonunun saptanması amaçlanmıştır. Hastalar ve Yöntem: Bu amaçla rozaseli 20 hastanın lezyonel deri örneklerinde ve gönüllü 5 hastanın nonlezyonel deri örneklerinde immünhistokimyasal metod ile östrojen ve progesteron reseptörleri çalışılmıştır. Bulgular: Lezyonel deri biyopsilerinden 2'sinde (10%) progesteron reseptör varlığı saptanmış, ancak hiç bir örnekte östrojen reseptörleri gösterilememiştir. Sonuç: Bu bulgular rozase etyolojisinde östrojen ve progesteron reseptörlerinin önemi hipotezini desteklememekle birlikte bloklanamayan/ karşı konulamayan androjenik uyarı hipotezinin araştırılması gerektiğini düşündürmektedir.Öğe Apoptosis-related proteins and steroid hormone receptors in normal, hyperplastic, and neoplastic endometrium(Lippincott Williams & Wilkins, 2002) Bozdoğan, Önder; Atasoy, Pınar; Erekul, Selim; Bozdoğan, Nazan; Bayram, MerihThe purpose of this study was to evaluate the distribution and frequency of apoptosis-related proteins and their correlation with estrogen, progesterone, and androgen receptors in endometrial tissues. Immunohistochemical analyses of bcl-2, bax, bcl-x, and steroid receptors were performed in 22 endometrial carcinomas, 26 endometrial hyperplasias, and 19 cases of normal cyclical endometrium. Bcl-2 was expressed in 45.4% of carcinomas and 92.3% of hyperplasias. Bax immunostaining was found in 90.9% of carcinomas and 76.9% of hyperplasias. Bcl-x positivity was similar in carcinomas (68.1%) and endometrial hyperplasias (76.9%). In nor-Mal cyclical endometria, bcl-2 staining was intense and diffuse in the proliferative phase, but decreased dramatically in the early and mid-secretory phase to reappear in the late secretory phase. Bax was expressed throughout the menstrual cycle but more strongly in the secretory phase. Bcl-x displayed a similar degree of expression in proliferative and secretory endometria. Nineteen carcinomas (86.3%), 25 hyperplasias (96.1%), and 18 normal cyclical endometria (94.7%) were positive for estrogen receptor (ER). Progesterone receptor (PR) was observed in 20 carcinomas (90.9%), all hyperplasias (100%), and 18 normal cyclical endometria (94.7%). Androgen receptor (AR) positivity was seen in 7 carcinomas (31.8%), 6 hyperplasias (23.0%), and 3 normal cyclical endometria (15.7%). There was a statistically positive correlation between bcl-x and ER and a tendency toward significant correlation between bcl-x and PR and between ER and PR in carcinomas. In hyperplasias, there was a significant positive correlation between bcl-2 and PR and between bcl-2 and bax and a negative correlation between ER and bax. There was a statistically significant difference for bcl-2 (p = 0.001) and bax (p = 0.001) between the hyperplasia and carcinoma groups. There was increased expression of bax. decreased expression of bcl-2, and persistence of bcl-x protein in advanced endometrial carcinomas. Our findings show that ovarian hormones have a regulatory role on bcl-2 protein and that there is a correlation between other members of the bcl-2 family (bcl-x and bax) and steroid hormone receptors. Bax/bcl-x may be the major control mechanisms of apoptosis in advanced carcinomas; other members of the bcl-2 family may also be under hormonal control.Öğe BAG-1 expression in hyperplastic and neoplastic prostate tissue: Is there any relationship with BCL-related proteins and androgen receptor status?(Sage Publications Ltd, 2005) Bozdoğan, Önder; Atasoy, Pınar; Bozdoğan, Nazan; Erekul, Selim; Batislam, Ertan; Yılmaz, Erdal; Basar, M. MuradAims and background: To evaluate the function and distribution of BAG-1 protein in hyperplastic and neoplastic prostate tissue and establish the relationship between this protein and BCL-related proteins (BCL-2 and BAX), androgen receptor (AR) expression and chromogranin A. Methods: Twenty-eight prostatic adenocarcinomas and 16 prostate hyperplasias were included in this retrospective study. BAG-1, BCL-2, BAX, androgen receptor and chromogranin A immunostaining was performed by means of standard avidin-biotin peroxidase methods. The M30 antibody was used to identify preapoptotic and apoptotic cells. The immunohistochemical histological score (HSCORE) semi-quantative system was used to evaluate immunohistochemical staining. Results: Statistical analysis showed a significant difference in HSCOREs of BAX, M30 and AR between the carcinoma and hyperplasia groups. Carcinomas expressed higher HSCOREs of these markers than hyperplasias. There were significant differences in nuclear and cytoplasmic BAG-1 positivity between high and low-grade carcinomas. BAG-1 expression was higher in low-grade carcinomas. In the carcinoma group there was a positive correlation (Pearson) between BCL-2 and cytoplasmic/nuclear BAG-1. In the hyperplasia group there was a negative correlation between BAX and BCL-2, and between AR and M30. We also detected a positive correlation between AR and nuclear/cytoplasmic BAG-1 and between nuclear and cytoplasmic BAG-1 in hyperplasias. BAG-1 showed the same specific basal cell localization as BCL-2 in hyperplastic and normal glands. Conclusions: The BAG-1 protein showed a distinct distribution pattern in hyperplastic and neoplastic prostate. BAG-1 in association with BCL-2 inhibits apoptosis and may prolong the life of neoplastic cells and give them a chance to gain new oncogenic features in early carcinogenesis.Öğe Bazal hücreli karsinomlarda metastaz baskılayıcı genler ve proteinler(2011) Bozdoğan, Önder; Çavuşoğlu, Tarık; Yuluğ, Önder; Vargel, İbrahim[Abstract Not Available]Öğe Bazal Hücreli Karsinomun Ender Yerleşim Alanları: Alt Dudak ve Popliteal Alan(2016) Sarı, Elif; Bekircan, Kağan; Bozdoğan, ÖnderBu yazıda alt dudak ve popliteal bölgede bazal hücreli karsinoma (BHK) tespit edilen iki olguyu sunduk. Kliniğimize 45 yaşında alt dudakta iyileşmeyen yarası olan ve 38 yaşında popliteal deri üzerinde iyileşmeyen yarası olan iki erkek hasta başvurdu. Öykülerinde güneşe maruz kalma ve travma olmayan her bir olgudan biyopsi alındı. Sonuç BHK olarak raporlandı. BHK genellikle baş ve boyun bölgesinde gözlenir, fakat ayrıca alt dudak ve alt ekstremite gibi nadir alanlarda da yerleşebilir. Bu öngörülemeyen alanlar tümör cerrahisi açısından akılda tutulmalıdırlarÖğe Clinical and histopathological findings in Bannayan-Riley-Ruvalcaba syndrome(Mosby, Inc, 2005) Erkek, Emel; Hizel, Selda; Şanlı, Cihat; Erkek, A. Bülent; Tombakoglu, M; Bozdoğan, Önder; Akarsu, CengizBannayan-Riley-Ruvalcaba syndrome is a rare autosomal dominant genodermatosis with the classical triad of macrocephaly, genital lentiginosis, and intestinal polyposis. Characteristic mucocutaneous manifestations include vascular malformations, lipomatosis, speckled lentiginosis of the penis or vulva, facial verrucae-like or acanthosis nigricans-like lesions, and multiple acrochordons of the neck, axilla, and groin. We present a case of Bannayan-Riley-Ruvalcaba syndrome with macrocephaly, abnormal facies, lipoma, tender and painful arteriovenous hemangiomas, lymphangiokeratomas, musculoskeletal abnormalities, and localized myopathy. We also describe previously unreported findings, including peripheral neuropathy, punctate cystic changes in acral tubular bones, and enostosis of talus. Bannayan-Riley-Ruvalcaba syndrome needs recognition by dermatologists because affected patients may present with mucocutaneous and subcutaneous lesions that may simulate other dermatological disorders.Öğe Deneysel Tek Taraflı Üreter Obstrüksiyonu Oluşturulan Ratlarda Dimetilsülfoksit Ve Pirasetam’ın Böbrek Hasarına Etkisi(2018) Yuvanç, Ercan; Tuğlu, Devrim; Kısa, Üçler; Bozdoğan, Önder; Bakar, Bülent; Batislam, Ertan; Yılmaz, ErdalAmaç: Dimetil Sülfoksit (DMSO) ve Pirasetam’ın deneysel unilateral üreter obstrüksiyonu (UÜO) oluşturulan ratlarda böbrek hasarını azaltıcı etkilerinin incelenmesi. Gereç ve Yöntem: Çalışma her biri 6 Sprague-Dawley rattan oluşan 4 deney grubunda yürütüldü. Grup 1: sham, Grup 2: UÜO (kontrol grubu), Grup 3: UÜO DMSO 3.8 g/kg grubu, Grup 4: UUO Piracetam 500 mg/kg grubu olarak tanımlandı. Total antioksidan kapasite (TAK) ve total oksidan seviye (TOS) ölçümleri ve histopatolojik inceleme için doku ve kan örnekleri alındı. Doku örnekleri histopatolojik olarak da incelendi. Bulgular: Biyokimyasal ve histopatolojik böbrek hasarı incelendi. Doku Total Antioksidan Kapasite (TAK) düzeyleri değerlendirildiğinde Grup 1 ve Grup 2’ye göre Grup 3 ve 4’de istatiksel olarak anlamlı bir artış olduğu görüldü. Grup 3 ve Grup 4 arasında ise istatiksel olarak anlamlı bir artış olduğu saptandı (p0.001). Doku Total Oksidan Seviye (TOS) değerleri incelendiğinde Grup 1 ve Grup 2’ye göre Grup 3 ve Grup 4’de oksidan düzeyinde istatiksel olarak anlamlı bir azalma olduğu; aynı zamanda Grup 3 ve Grup 4 arasında da istatiksel olarak anlamlı bir azalma olduğu saptandı (p0.001). Doku Oksidatif Stres Indeksi (OSI) parametresi incelendiğinde Grup 1 ve Grup 2’ye göre Grup 3 ve 4’de OSI değerlerinde istatiksel olarak anlamlı bir azalma olduğu tespit edildi. Histopatolojik inceleme de böbrek dokusunda Grup 2’ye göre Grup 3 ve 4’te histopatolojik olarak istatistiksel olarak bir fark saptanmadı. Sonuç: Dokuda biyokimyasal düzeyde DMSO ve Pirasetam’ın antioksidan etkili olduğu aynı etkinin histolojik olarak koruyucu etkinlik oluşturmadığı saptanmıştır. Bununla birlikte bu ilaçların farklı doz ve sürelerle yapılacak çalışmalar ile doku antioksidan özelliklerinin saptanabileceği düşünülmektedir.Öğe Deneysel Testis Torsiyonu Sonrası İskemi/Reperfüzyon Hasarında İloprost Ve Düşük Doz Metotreksat’ın Koruyucu Etkisi(2017) Yuvanç, Ercan; Tuğlu, Devrim; Kısa, Üçler; Bozdoğan, Önder; Batislam, Ertan; Yılmaz, ErdalAmaç: Testis torsiyonunda cerrahi olarak detorsiyone edilen testiste gelişen iskemi/reperfüzyon (I/R) hasarı üzerine İloprost ve düşük doz Metotreksat’ın koruyucu etkilerini araştırmak. Gereç ve Yöntem: Ratlar rastgele 4 gruba ayrıldı. Grup 1 (sham), Grup 2 İskemi/Reperfüzyon, ilaç verilmedi. Grup 3 İskemi/Reperfüzyon İloprost (10 µg/kg) ve Grup 4 İskemi/Reperfüzyon Düşük doz Metotreksat (6 mg/kg). Torsiyon/iskemi ve detorsiyon/reperfüzyon süreleri 4’er saat olarak belirlenmiştir. Medikasyonlar intraperitoneal yolla testis torsiyonunu takiben 3. saatte verilmiştir. Her 4 grupta işlemin başlangıcından 8 saat sonra detorsiyone sağ testis çıkarılmıştır. Bulgular: Çalışmamızda, iloprost ve düşük doz metotreksat verilmesinin total antioksidan kapasiteyi (TAK) istatistiksel olarak anlamlı arttırdığını, oksidan kapasite markırı olan total oksidan kapasite (TOK) ve oksidatif stres indeks oranını (OSI) ise istatistiksel olarak anlamlı düzeyde azalttığını belirledik. İskemireperfüzyon grubu testislerde testis grupları arasında Johnsen skorlama sistemine göre istatistiksel farklılık saptanmadı. Sonuç: Bizim düşüncemize göre iloprost ve düşük doz metotreksat tedavisi, testis örneklerinde antioksidan aktiviteyi arttırmış, oksidan aktiviteyi de baskılamıştır. Ancak, histopatolojik incelemede ve spermatogenes skorlarında bir değişime neden olmamıştır.Öğe Diffuse hemangiomatosis of the spleen associated with pregnancy: A case report(2004) Atasoy, Pınar; Bozdoğan, Önder; Erekul, SelimWe report a 21-year-old postpartum woman presenting with a very rare tumor of the spleen, diffuse hemangiomatosis. Gross and microscopic examination revealed that the whole spleen was permeated by neoplastic blood vessels. Immunohistochemical staining suggested an origin from vascular endothelium (CD 31+, CD 34+, FVIII+ and CD 8-). Although steroid hormone receptors are discovered in other vascular proliferations, we report the first case of a diffuse hemangiomatosis of the spleen showing strong and diffuse immunopositivity for estrogen receptor (ER). Hormonal changes associated with pregnancy might have induced or promoted the development of diffuse splenic hemangiomatosis.Öğe The effect of extracorporeal shock wave treatment (ESWT) on bone defects: An experimental study(1999) Uslu, Murad M.; Bozdoğan, Önder; Güney, S.; Bilgili, Hasan; Kaya, Ü.; Olcay, B.; Korkusuz, FezaThe aim of this study was to investigate the effects of extracorporeal shock wave therapy (ESWT) on the formation of callus in bone defects created in rabbit radii. This study searches for an answer to whether ESWT may have a therapeutic effect on bone defects. A bone defect with a radius of 1 cm was created in both forelimbs of 20 rabbits. At the 7th, 14th, and 21st days ESWT treatment was applied to the forming callus in the right radius under fluoroscopic control. At the 6th and 12th weeks, the animals were sacrificed and callus analysis was performed by computerized scan, dual energy x-ray absorptiometer. Histological analyses were also performed. The results revealed that the average callus area in the right (ESWT applied) radial defect was greater in both groups and statistically significant at the 12th week (p < 0.05). There was no difference in bone density between defects. Histologically the callus area was greater on the right side (ESWT applied side) in both groups. However in the first group trabeculae were occupying less space on the right side. Granulation tissue areas and chondroid areas were greater on the right side. We conclude that ESWT has a disorganizing and dispersing rather than a direct osteoinductive effect on forming callus. This effect may play a therapeutic role in bone defects and in situations like callus lengthening where a greater amount of callus is necessary.Öğe Effect of slow-release 5-fluorouracil on capsule formation around silicone breast implants: An experimental study with mice(Springer, 2007) Canter, Halil İbrahim; Konas, Ersoy; Bozdoğan, Önder; Vargel, İbrahim; Özbatir, Bilgen; Öner, Filiz; Erk, YücelBackground: Capsule formation around breast implants, development of tendon adhesions after tendon repair, intestinal brits after laparatomies, hypertrophic scars in skin incisions all are the results of excessive collagen synthesis to the extracellular matrix by fibroblasts. Any intervention that leads to cessation of collagen synthesis in these clinical situations may help to prevent these untoward results of wound healing. Although 5-fluorouracil (5-FU) is used mainly as a cytotoxic drug in chemotherapy protocols, it decreases cellular metabolism and blocks protein synthesis only at lower concentrations. Findings have shown that 5-FU downregulates fibroblast proliferation and differentiation in vitro. It has been used to treat fibroproliferative disorders of the eye and skin and is thought to inhibit thymidylate synthetase, blocking DNA replication. Methods: This study used five treatment groups: (1) gelatin only, (2) silicone only, (3) silicone + gelatin, (4) silicone + gelatin containing 1 mg of 5-FU, and (5) silicone + gelatin containing 5 mg of 5-FU. The release kinetics of 5-FU from gelatin have been investigated by means of ultraviolet spectrophotometric analysis. Specimens were obtained on postoperative day 30. Gross evaluation and histopathologic examination were conducted for capsule formation and the development of inflammation. Results: The silicone group had the most prominent capsule formation among all the groups. The gelatin group was second, and the silicone + gelatin group was third. As compared with the other groups, the 5-FU-containing groups had the least capsule formation. The 5-mg 5-FU-containing group had the most inflammation. The silicone + gelatin group was second in inflammation. Although the silicone, gelatin, and 1-mg 5-FU-containing groups had the same means, the results of the silicone group showed the most divergent data within the group. Conclusions: Because 5-FU loaded to a gelatin carrier for its slow release seems to prevent capsule formation around silicone blocks, it may be used to prevent capsule formation around silicone breast implants.Öğe The effects of hormone therapy, estrogen therapy and tibolone on apoptosis and cyclin D-1 expression in postmenopausal vaginal epithelium(Elsevier Science Bv, 2005) Sağsöz, Nevin; Yücel, Aykan; Noyan, Volkan; Bozdoğan, ÖnderObjective: To investigate the effects of hormone therapy, estrogen therapy and tibolone on markers of apoptosis including bcl-2, and bax and cyclin D, expression in postmenopausal vaginal epithelium. Study design: Thirty postmenopausal women were randomized to the treatment protocols (0.625 mg conjugated equine estrogen (CEE) + 2.5 mg medroxyprogesterone acetate (MPA); 2 mg estradiol valerate; 2.5 mg tibolone). After baseline vaginal biopsy, control biopsies were performed after 70 days following the initiation of the therapy. Bcl-2, bax, Bcl-2/bax ratio, cyclin D, measurements were performed immunohistochemically. Data were analyzed by Kruskal-Wallis, Mann-Whitney U and Wilcoxon tests. Results: After the treatment period the above-mentioned parameters were not different among the groups except for cyclin D-1 levels. Cyclin D-1 expression was found to be strong in patients with treated estradiol valerate. Conclusions: The effects of estrogen on cyclin D-1 expression were not detected with tibolone or with the addition of progesterone to estrogen in the vaginal epithelium. Cyclin D, appeared to have stronger effects on the estrogen related proliferation compared to apoptotic markers in vaginal epithelial cells. (c) 2004 Elsevier Ireland Ltd. All rights reserved.Öğe Endometrial hiperplazilerde epitelyal sitoplazmik değişiklikler (metaplaziler)(2001) Bozdoğan, Önder; Bozdoğan, Nazan; Atasoy, PınarAMAÇ:"Endometriyumun epitelyal sitoplazmik değişiklikleri (metaplaziler)" (EESD) genellikle hiperöstrojenik klinik durumlarda ortaya çıkan bir grup lezyonu tanımlar. EESD'ler prognostik öneme sahip olmamalarına rağmen yanlış tanılara neden olabilirler. Bu çalışmanın amacı endometrial hiperplazilerde EESD'lerin sıklığının ve öneminin araştırılmasıdır. YÖNTEM: Johannes Gutenberg Üniversitesi (Mainz -Almanya) Patoloji Enstitütüsü ve Kırıkkale Üniversitesi Tıp Fakültesi Patoloji Anabilim Dalı arşivlerinden derlenen endometrial hiperplazi tanısı almış 27 olguya ait 23 adet küretaj ve 7 adet histerektomi materyali iki patolog tarafından yeniden değerlendirildi. SONUÇLAR: Olguların, 12'si kompleks atipili, 7'si kompleks atipisiz, 8'i basit atipisiz hiperplazi tanılarını almıştı. Toplam 17 olguda metaplastik değişiklik saptandı. Olguların 8'inde fokal silyalı, 4'ünde yüzey sinsityal, 1'inde skuamoz ve 1'inde müsinöz değişiklik izlendi. Üç olguda silyalıyüzey sinsityal değişiklik, yüzey sinsityaleosinofilik değişiklik, skuamozsilyaiı değişiklik birlikteliği görüldü. YORUM: Bu çalışmada, EESD'lerin endometrial hiperplazilerde beklediğimizden sık görüldüğünü saptadık. Ayrıca bu lezyonların, deneyimsiz gözlemcilerde önemli tanısal sorunlara yol açabileceğini düşünmekteyiz.Öğe Expression profile and prognostic importance in prostate lesions of the reverse transcriptase component of human telomerase (hTERT) and of cyclin-dependent kinase inhibitor p57 (p57kip2a)(Springer, 2009) Atasoy, Pınar; Yılmaz, Erdal; Bozdoğan, Önder; Ayva, Şebnem; Batislam, ErtanTo investigate expression of the reverse transcriptase component of human telomerase (hTERT) and of cyclin-dependent kinase inhibitor p57 (p57(kip2a)) in prostate neoplasms and evaluate the relationship between these proteins and the Gleason score. hTERT and p57(kip2a) antibodies were studied by immunohistochemical methods in 70 prostate adenocarcinomas (33 high-grade and 37 low-grade carcinomas), 29 benign prostate hyperplasias, and 19 prostatic intraepithelial neoplasias (PIN). Only nuclear staining was evaluated with p57(kip2a) whereas both nuclear and nucleolar staining were evaluated with hTERT. Immunohistochemical histologic scores (HSCOREs) of hTERT were significantly higher in the PIN group than in the hyperplasia group (P = 0.03). hTERT HSCOREs were not significantly different between hyperplasias and carcinomas or between low and high-grade carcinomas. p57(kip2a) HSCOREs were significantly higher in hyperplasias than other groups, and in PINS than carcinomas, but did not differ significantly between low and high-grade carcinomas. A significant negative correlation was observed between hTERT and p57(kip2a) (P = 0.007) in the hyperplasia group. No such correlation was found in PINs and carcinomas. This study suggests that p57(kip2a) is down-regulated in the malignant side of the spectrum of prostate carcinogenesis. Loss of p57(kip2a) control on hTERT might have an important role in the development of prostate cancer.Öğe FAS, FAS ligand, tumor infiltrating lymphocytes, and macrophages in malignant melanoma: an immunohistochemical study(Wiley, 2010) Bozdoğan, Nazan; Bozdoğan, Önder; Pak, Işın; Atasoy, PınarBackground FAS and its ligand, FASL, have important roles in the neoplasia-immunity relationship. In melanoma, the importance of FAS and FASL remains controversial, despite a group of studies. In this study, we aimed to demonstrate the distribution of FAS/FASL in melanotic lesions and to investigate the correlation between tumor infiltrating lymphocytes and macrophages. Methods Ten intra-dermal nevi, 12 primary malignant melanoma, and eight skin and 15 lymph node metastases were included in this study. FAS and FASL were studied in all of the groups using classical labeled streptavidin-biotin immunohistochemical method. Tumor infiltrating lymphocyte status and macrophage number demonstrated by CD68 immunostain were also evaluated in primary melanoma and skin metastases. Results FAS positivity was detected in all of the cases. FASL expressions were seen in 60% of the intra-dermal nevus and in all of the other groups. There were significant differences in FASL between nevus and primary melanoma, nevus and skin metastasis, and nevus and lymph node metastasis. There were strong positive correlations between FAS expression and intra-neoplastic macrophage score and between FASL and density of lymphocyte infiltration in skin metastases. Conclusion Although FAS and FASL expression is a constant feature of melanotic lesions, its diagnostic importance is very limited because of the different results obtained in the past studies. The correlation between FAS status and macrophage number and between FASL status and lymphocyte number in skin metastasis but not in primary lesions might point to diverse FAS/FASL interaction between neoplastic cells and macrophages in the different microenvironments.Öğe Fox-Fordyce hastalığı(2002) Erkek, Emel; Koçak, Mukadder; Atasoy, Pınar; Birol, Ahu; Bozdoğan, ÖnderFox-Fordyce hastalığı nadir görülen, sporadik, apokrin ter bezi retansiyonu sonucu oluşan, kaşıntılı, foliküler papüllerle karakterize bir dermatozdur. Patogenezindeki en önemli basamak apokrin ter bezi duktuslarının tıkanması ve rüptürüdür. Bu makalede, Fox-Fordyce tanısı alan bir olgu sunulmuş ve literatür ışığında klinikopatolojik özellikleri tartışılmıştır.Öğe Giant condyloma acuminata of Buschke-Lowenstein: successful treatment with a combination of surgical excision, oral acitretin and topical imiquimod(Wiley-Blackwell Publishing, Inc, 2009) Erkek, Emel; Başar, Halil; Bozdoğan, Önder; Emeksiz, M. CanHuman papillomavirus (HPV) is the most common sexually transmitted disorder in young, sexually active populations in the USA and Europe. Giant condyloma acuminatum (GCA) is a unique variant of condyloma acuminata, characterized by local aggressive behaviour despite benign histology. It carries a substantial risk of squamous cell carcinoma. Various treatments have been used, but response is often poor and recurrence rates high. We present a case of GCA successfully treated with a combination of surgical excision, oral acitretin and topical imiquimod. The diagnosis was based on histological examination, immunohistochemical analysis and in situ hybridization for HPV 6 and 11. We recommend a trial of oral retinoid and topical imiquimod in selected cases of GCA.
