Yazar "Kalender, H." seçeneğine göre listele
Listeleniyor 1 - 12 / 12
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Concurrent peste des petits ruminants virus and pestivirus infection in stillborn twin lambs(Amer Coll Vet Pathologist, 2008) Kul, O.; Kabakci, N.; Ozkul, A.; Kalender, H.; Atmaca, H. T.Concurrent infection with peste des petits ruminants virus (PPRV) and pestivirus was diagnosed in stillborn twin lambs. With the flock history, the findings of epidermal syncytial cells and necrotizing bronchitis/bronchiolitis prompted testing for PPRV infection, and PPRV antigen was detected by immunohistochemistry (IHC) in the skin, lungs, kidneys, rumen, and thymus. Macroscopic anomalies that were typical of border disease included scoliosis, brachygnathism, prognathism, arthrogryposis, hydranencephaly, cerebellar hypoplasia, and hairy fleece; pestiviral antigen was detected by IHC in the brain, liver, lungs, and kidneys. Tissues from both lambs were positive by reverse transcriptase-polymerase chain reaction (RT-PCR) for PPRV and pestivirus. To the authors' knowledge, PPR has not been reported previously as a congenital infection or in combination with pestiviral infection.Öğe Congenital chondrodysplasic dwarfism in lambs: A diferent consideration of dystocia(Israel Veterinary Medical Assoc, 2010) Yagci, Pir, I; Kul, O.; Kalender, H.; Polat, I. M.In this study, we report three chondrodysplasic anomalous lambs obtained from 2 Akkaraman sheep with dystocia. According to the flock history, the incidence of congenital skeletal anomalies among lambs was 14.28 % and 11.66 % in 2005 and 2006, respectively. The same two rams had been used to obtain natural mating and no new animals had been bought into the flock over the last 5 years. Affected lambs showed typical macroscopic features of a compressed and flattened face, domed head, narrow thorax, swollen abdomen and extremely short paddle like extremities. Radiological examination of extremities of the long bones revealed that diaphyses were extremely shortened and distance between diaphysis and epiphysis was increased. Long bones such as antebrachium, humerus, tibia and femur were short and thick. Histopathologically, immature chondrocytes were present at the articular surface and defective epiphyseal plates were detected in the extremities of the bones and sternum. Persistent cartilage showed irregular strands containing groups of two or four chondrocytes localized in the basophilic matrix. In conclusion, chondrodysplasia of lambs should be added to the list of differential diagnoses of dystocia especially in inbreed sheep.Öğe Distribution and heterogeneity of mast cells in female reproductive tract and ovary on different days of the oestrus cycle in Angora goats(Wiley-Blackwell, 2008) Karaca, T.; Arikan, S.; Kalender, H.; Yoruk, M.The physiological distribution of mast cells (MCs) in the reproductive tract and ovary of 12 Angora goats was determined using light microscopic histochemical techniques. Uterus (corpus uteri and cornu uteri), uterine cervix, uterine tubes (isthmus and ampulla) and ovary samples were obtained by laparatomy from groups of animals during metoestrus, dioestrus and proestrus (days 5, 10 and 16 of the oestrous cycle). Tissues were fixed in Mota's fixative (basic lead acetate) for 48 h and embedded in paraffin. Six-micrometre-thick sections were stained with toluidine blue in 1% aqueous solution at pH 1.0 for 5 min and alcian blue-Safranin at pH 1.0 for 30 min. MCs were generally associated with blood vessels in all reproductive organs. In the uterus, they were concentrated mainly in the close of the uterine gland and deep stroma in the endometrium. Higher MC numbers were observed by toluidine blue staining in the uterus, uterine cervix and uterine tubes on days 10 (corpus uterine: 4.7 +/- 3.8 and cornu uterine: 4.9 +/- 3.5) and 16 (corpus uterine: 5.9 +/- 4.5 and cornu uterine: 5.4 +/- 2.4) of the oestrous cycle compared with day 5 (p < 0.05). Mast cells were not observed in the follicles, the corpus luteum and the underside of the surface epithelium of the ovarian cortex, but were observed in the interstitial cortical stroma and the ovarian medulla. In the ovary, MC numbers were significantly higher on day 16 of the oestrous cycle (cortex: 3.4 +/- 2.4 and medulla: 5.7 +/- 4.5, p < 0.05). Safranin-positive connective tissue MCs were not observed in the uterine tube on any occasion. These results indicate oestrous cycle-related changes in the number and location of MCs in goat reproductive organs.Öğe Does serum anti-Mullerian hormone levels always discriminate presence of the ovaries in adult bitches? Comparison of two ELISA kits(Wiley-Blackwell, 2016) Yagci, I. Pir; Pekcan, M.; Polat, I. M.; Kalender, H.; Macun, H. C.Anti-Mullerian hormone (AMH) is produced in the ovary, and thus, it is an excellent marker of follicle pool in females. Current interest is the clinical use of this parameter as a biomarker to assess presence or absence of an intact ovary and to diagnose ovarian remnant syndrome (ORS) following incomplete ovariohysterectomy (OHE) in bitches. The aim of this study was to evaluate serum AMH concentrations in bitches (n = 34) before and after OHE using two different commercial ELISA kits, one of which is based on detecting human AMH and the other is based on detecting human AMH and the other specified for canine AMH. Furthermore, serum AMH levels were also measured in six ORS cases to compare the diagnostic utility of the two different ELISA kits. Serum AMH concentrations measured using the human and canine kit prior to and after OHE were 0.32 +/- 0.24, 0.006 +/- 0.22 ng/ml (p<.001) and 12.08 +/- 22.81, 9.55 +/- 15.42 ng/ml (p=.868), respectively. Thus, the canine-based kit was not able to reveal the significant drop in serum AMH levels. In conclusion, the human-based ELISA kits successfully detected the drop in serum AMH concentrations. Reliable results can only be achieved from well-designed ELISA kits, and AMH levels might be a useful diagnostic tool for the evaluation of presence or absence of ovaries as well as for the detection of ORS cases in bitches.Öğe Effects of Cholesterol on Progesterone Production by Goat Luteal Cell Subpopulations at Two Different Stages of the Luteal Phase(Wiley-Blackwell Publishing, Inc, 2010) Arikan, S.; Kalender, H.; Simsek, O.Contents The aim of the present study was to evaluate the effects of cholesterol on progesterone production during long-term culturing of luteal cell subpopulations at early and late luteal stages of the goat corpora lutea. Corpora lutea were collected from Angora goats on days 5 and 15 of the oestrous cycle. Luteal cells were isolated by collagenase digestion. The cells were separated into two distinct subpopulations by Percoll density-gradient centrifugation. Both subpopulations of luteal cells staining positively for 3 beta-HSD activities (5 x 104 cell/well) were cultured with or without 22(R)-hydroxycholesterol (22R-HC) in serum-free culture medium for periods of up to 7 days. Cells were incubated with serum (10%) for the first 18 h of incubation followed by serum-free medium. Cell treatment (10 and 20 mu g/ml) was performed on days 1, 3 and 5. Treatment of cells with both concentrations of 22R-HC resulted in significant (p < 0.01) and dose-dependent stimulation (p > 0.05) on progesterone production in both fractions of cells throughout 7 days of incubation. Treatment of the cells with cholesterol resulted in 2.5- and 9.0-fold increases in progesterone accumulation on day 3 of incubation. Steroid production was maintained throughout the incubations when cells are incubated in serum-free media treated with cholesterol and ITS premix. Cells collected from higher density of percoll layers produced 2.82 and 2.32 times more progesterone, in comparison to the lover density percoll layer, on days 5 and 15 of the oestrous cycle in untreated cell groups, respectively. Progesterone accumulation was decreased as incubation time advanced in all groups of untreated cells. These results demonstrated that goat luteal cell subpopulations secrete substantial amounts of progesterone in response to cholesterol treatment at least for 7 days, and cholesterol is required as progesterone precursor for maintaining a high-level steroidogenesis during long-life culturing of both cell subpopulations.Öğe Effects of dbcAMP on progesterone synthesis by cultured goat luteal cell subpopulations isolated from early and late luteal stage corpora lutea(Brazilian Coll Animal Reproduction, 2016) Arikan, S.; Kalender, H.; Simsek, O.This research aimed to investigate the effects of dbcAMP on steroid accumulation by culturing two distinct luteal cell subpopulations isolated from early and late luteal stage corpora lutea. Cells were isolated from corpora lutea collected from eight Angora goats on either the 5th or 15th days of their estrous cycles. Cell isolation was performed by enzymatic digestion using collagenase and DNase. Isolated cells were separated into two distinct subpopulations enriched with small and large luteal cells by percoll density-gradient centrifugation. Isolated cells were stained in order to detect 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD). Cells stained positively for 3 beta-HSD activity (5 x 10(4) cell/well) were incubated with dbcAMP in the absence or presence of 22(R)-hydroxycholesterol (22R-HC) for periods of up to 7 days. Large luteal cell enriched subpopulations produced more basal progesterone (P < 0.05) than did the small luteal cell enriched subpopulations. Treatment of cells with 22R-HC alone induced 4.00 to 11.60 times increase in steroid synthesis depending on type of cells incubated, luteal age and days of incubation. Incubation of cells with 1 mM dbcAMP in the absence or presence of 22R-HC induced in a significant increase (P < 0.01) in steroid accumulation in all treated groups. In contrast, when cells are treated with low dose dbcAMP (0.1 mM), treatment induced stimulation failed to reach significant level in most treated groups. In conclusion, although treatment of goat luteal cells with dbcAMP induces an increase in steroid accumulation, a high dose is necessary to reach significant levels. Stimulatory effect of dbcAMP on steroidogenesis maintains during long life culturing.Öğe Effects of exposure to new inhalational anesthetics on spermatogenesis and sperm morphology in rabbits(Taylor & Francis Inc, 2005) Ceyhan, A.; Cincik, M.; Bedir, S.; Üstün, H.; Dağlı, G.; Kalender, H.The effects of chronic exposure to new inhalation anesthetics (sevoflurane and isoflurane) on spermatogenesis and sperm morphology were examined in 23 rabbits, randomly divided in 3 groups. Rabbits received 20 exposure hours (four hours/day x 5 days), as follows: group I: 2.3% (1.2 MAC) sevoflurane+2L/min oxygen, group II: 1.3% (1.2 MAC) isoflurane+2L/min oxygen, and group III (control): 2L/min oxygen. Semen was collected on the 12th, 19th, 26th, 33rd, and 41st days of exposure. Sperm concentration, motility and morphological changes were evaluated. On the 41st day, testicular biopsies were taken and observed with light microscopy. Sperm concentration and motility significantly decreased in the sevoflurane and the isoflurane groups, compared to control. There were no significant changes in the control group. It is concluded that chronic exposure to the new inhalational anesthetics had negative effects on spermatogenesis and sperm morphology.Öğe The effects of raloxifene and tibolone on homocysteine and vascular histopathological changes(Springer-Verlag Italia Srl, 2007) Bayram, M.; Özer, G.; Kalender, H.; Kabakcı, N.; Kısa, Üçler; Özkan, Y.The aim of the present study was to examine the effects of raloxifene (RLX) and tibolone (TBN) on plasma homocysteine (Hey) levels and their relationship with atherosclerotic changes in the walls of the carotid artery in ovariectomised rats. Thirty surgically ovariectomised Wistar albino rats after a menopausal period of 6 cycles were randomly assigned to receive RLX 0.01 mg/kg/day (n=10), TBN 0.04 mg/kg/day (n=10) and the same dose of placebo (n=10) for 6 cycles. Serum levels of vitamin B12, folate and Hey were measured and carotid arteries were examined histopathologically following the termination of treatment. Hey levels were 3.27 +/- 0.97, 2.57 +/- 0.32 and 2.28 +/- 0.12 mu mol/l, Vitamin B12 levels were 901.90 +/- 239.76, 694.70 +/- 112.20 and 631 +/- 309.44 pg/ml and folate levels were 73.80 +/- 12.71, 72.51 +/- 7.05 and 84.79 +/- 20.82 ng/ml in receiving RLX, TBN and placebo respectively. Hey levels were increased by RLX vs. placebo (P=0.006) but not by TBN vs. placebo (P=0.070). Vitamin B 12 levels were found to be elevated by TBN vs. the control group (P=0.041) but not by RLX vs. placebo (P=0.059). Histopathological examination of carotid arteries from rats receiving both RLX and TBN revealed no difference vs. placebo. Data obtained from the study support the view that neither RLX nor TBN appears to have a primary protective effect on vascular disease by effecting the metabolism of Hey at menopause.Öğe The relationship between the stages of the sexual cycle, the pregnancy and postpartum periods and vaginal flora in Kangal Breed bitches(Scientific Technical Research Council Turkey, 2003) Findik, M.; Maral, N.; Keskin, O.; Kalender, H.; Erdeger, J.; Aslan, S.This study was performed on females of the Kangal Breed (Turkish Anatolian Shepherd Dog) with the aim of determining the relationship between the stages of the sexual cycle and vaginal flora. The stages of the cycle in Kangal bitches (n = 16) were determined by performing vaginoscopic, vaginal cytologic and ultrasonographic checks at regular intervals and examining vaginal swab samples bacteriologically. The swab samples were inoculated onto 7% sheep blood agar and incubated for 18-24 h at 37 degreesC and total counts of the resulting colonies were evaluated. In addition, inoculations were made onto 7% sheep blood agar, PPLO agar, Columbia agar and Brucella agar with serum and incubated in a 10% CO2 environment at 37degreesC and onto Saboraud dextrose agar, and these were incubated at room temperature and the resulting colonies identified. In all stages of to cycle E. coli and also Streptococci, were isolated, except in the pregnancy period. It was determined that haemolytic streptococci grew at the estrus and early metestrus stages while alpha-haemolytic streptococci grew at other stages of the cycle. Staphylococci were not found at the pre-proestrus and estrus stages, whereas they were isolated in various percentages from samples taken at the other stages. At the stages following the end of estrus, Citrobacter, Flavobacter and Proteus spp. were isolated in different percentages. Enterobacter, Acinetobacter and Neisseria spp. were isolated at different stages of estrus, Pasteurella spp. at late estrus and Shigella spp. as well as yeast in the pre-proestrus periods. While the E coli was grown at a rate of 76.9% from the samples from breeding bitches, the coincidence rates of B-haemolytic streptococci, alpha-haemolytic streptococci as well as S. aureus were determined to be 15.4%, 15.4% and 38.5%, respectively. Pasteurella, Neisseria and S. epidermidis levels were the same; 7.7%. It was observed that, E. coli appeared at a level of 100% in all the non-pregnant bitches, and E. coli alone was isolated at a level of 75%. Similarly E. coli appeared in six of the bitches that became pregnant (66.7%) and E. coli was isolated alone from half of these samples (50%). As a result it was observed that some of the bacterial components comprising the vaginal flora were present at every stage of the sexual cycle, while some were absent at particular stages; bacteria could grow at the estrus stage in the vagina without influencing the outcome of the pregnancy.Öğe Size distribution of dispersed luteal cells during oestrous cycle in angora goats(Blackwell Publishing, 2007) Kalender, H.; Arıkan, S.The present study examines the size distribution of the goat steroidogenic luteal cells throughout the oestrous cycle. Corpora lutea (CL) were collected after laparatomy on days 5, 10 and 16 of the oestrous cycle. Luteal cells were isolated from CL by collagenase digestion. Steriodogenic luteal cells were identified by staining of the cells for 3 beta-hydroxysteroid dehydrogenase activity, a marker for steroidogenic cells. Luteal cells having steroidogenic capacity covered a wide spectrum of sizes, ranging from 5 to 37.5 mu m in diameter. There was a significant increase in mean cell diameters (p < 0.01) as CL aged. The mean cell diameter on day 5 was 11.55 +/- 0.12 mu m, which was significantly increased and reached up to 19.18 +/- 0.24 mu m by day 16 of the oestrous cycle. The ratio of large to small luteal cells was 0.06:1.0 on day 5 of the oestrous cycle. This ratio increased to 0.78:1.0 by day 16 of the oestrous cycle. Luteal cell size on days 5, 10 and 16 of the oestrous cycle reached its maximum at 7.5, 10 and 35 mu m in diameter, respectively. Development of CL is associated with an increase in luteal cell size in goats. It is likely that small luteal cells could develop into large luteal cells as CL becomes older during oestrous cycle in goats.Öğe Size Distribution of Luteal Cells During Pseudopregnancy in Domestic Cats(Wiley, 2009) Arikan, S.; Yigit, A. A.; Kalender, H.Contents Experiments were designed to investigate the size distribution of queen steroidogenic luteal cells throughout pseudopregnancy. Corpora lutea were obtained from the queens following ovariohysterectomy on days 7, 15 or 25 of pseudopregnancy. Luteal cells were isolated from the ovary by collagenase digestion. Steriodogenic cells were identified by staining of cells for 3 beta-HSD activity. Cell diameters were measured using a microscope. Luteal cells having steroidogenic capacity covered a wide spectrum of sizes ranging from 3 to 35 mu m in diameter. There was a significant increase in mean cell diameters (p < 0.01) as pseudopregnancy progressed. Mean diameter of 3 beta-HSD positive cells increased from 10.41 +/- 0.7 mu m, on day 7 of pseudopregnancy, to 19.72 +/- 1.3 mu m on day 25 of pseudopregnancy. The ratio of large (> 20 mu m in diameter) to small (3-20 mu m in diameter) luteal cells was 0.08 : 1.0 on day 7 of pseudopregnancy, with the 7.5-10 mu m cell size class predominant. By day 25 of pseudopregnancy, the ratio of large-to-small cells was increased to 0.87 : 1.0, and 20-25 mu m cell sizes become predominant. In conclusion, this study has demonstrated that the cells of the corpus luteum undergo continuous differentiation during pseudopregnancy in queen. This study also demonstrates that luteal cells dissociated from pseudopregnant queen can be used as a model to study the physiology of corpus luteum in pregnant cats.Öğe Total oxidant and antioxidant activities in milk with various somatic cell count intervals during discrete cow and buffalo lactation periods(Wiley, 2019) Yigit, A. A.; Cinar, M.; Macun, H. C.; Ozenc, E.; Kabakci, R.; Yazici, E.; Kalender, H.[Abstract No tAvailable]
