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Öğe Comparison of TLR-2, TLR-4, and antimicrobial peptide levels in different lesions of acne vulgaris(Taylor & Francis Ltd, 2016) Ozlu, Emin; Karadag, Ayse Serap; Ozkanli, Seyma; Oguztuzun, Serpil; Kilic, Murat; Zemheri, Ebru; Akdeniz, NecmettinContext: Recent studies have shown that tolls like receptors (TLRs) and antimicrobial peptides (hBD-1, cathelicidin) play an important role in the pathogenesis of acne vulgaris (AV).Objective: To evaluate and report the expression of TLR-2, TLR-4, hBD-1 and cathelicidin in different regions of skin in AV.Participants: This study was performed in 80 patients with AV and a control group of 20 healthy individuals.Material and methods: Skin biopsies were performed from 20 papular, 20 pustular, 20 comedonal and 20 nodular lesions of patients and 20 healthy volunteers. Expression levels of TLR-2, TLR-4, hBD-1 and cathelicidin in four separate areas (epidermis, dermis, inflammation region and skin appendages) were evaluated by immunohistochemical method. Further, these parameters were compared between different skin lesions.Results: A significant difference was found between the levels of staining of TLR-2, TLR-4 and hBD-1 from the epidermis, inflammation region, dermis and skin appendages (p<0.05). Levels of cathelicidin were different in only the inflammation region (p<0.05). The level of TLR-2 in the epidermis with nodules was lower than the papules and comedones (p<0.05). Levels of TLR-2 in the inflammation and dermis of the cases with papules were significantly higher when compared to pustules (p<0.05). The levels of staining of TLR-4 in the dermis with comedones were significantly lower compared to the cases with papules (p<005). The level of hBD-1 in the epidermis region of comedones was significantly higher compared to nodules (p<0.05). The expression of cathelicidin in the inflammation region of comedones was significantly low (p<0.05).Conclusion: It is thought that TLR-2, TLR-4, hBD-1 and cathelicidin play an important role in the pathogenesis of AV and in the development of different acne types. We think that, better results could be obtained in treatment of AV with different treatment options targeted in regulation of TLR-2, TLR-4, hBD-1 and cathelicidin release.Öğe Cytotoxicity and apoptotic effects of nickel oxide nanoparticles in cultured HeLa cells(Via Medica, 2010) Ada, Kezban; Turk, Mustafa; Oguztuzun, Serpil; Kilic, Murat; Demirel, Mehmet; Tandogan, Nisa; Latif, OzturkThe aim of this study was to observe the cytotoxicity and apoptotic effects of nickel oxide nanoparticles on human cervix epithelioid carcinoma cell line (HeLa). Nickel oxide precursors were synthesized by an nickel sulphate-excess urea reaction in boiling aqueous solution. The synthesized NiO nanoparticles (<200 nm) were investigated by X-ray diffraction analysis and transmission electron microscopy techniques. For cytotoxicity experiments, HeLa cells were incubated in 50-500 mu g/mL NiO for 2, 6, 12 and 16 hours. The viable cells were counted with a haemacytometer using light microscopy. The cytotoxicity was observed low in 50-200 mu g/mL concentration for 16 h, but high in 400-500 mu g/mL concentration for 2-6 h. HeLa cells' cytoplasm membrane was lysed and detached from the well surface in 400 mu g/mL concentration NiO nanoparticles. Double staining and M30 immunostaining were performed to quantify the number of apoptotic cells in culture on the basis of apoptotic cell nuclei scores. The apoptotic effect was observed 20% for 16 h incubation.Öğe Determination of the Surface Roughness Values of Turkish Red Pine (Pinus brutia (Ten.)) Woods(North Carolina State Univ Dept Wood & Paper Sci, 2017) Kilic, MuratThe aim of this study was to determine the surface roughness values of Turkish red pine samples obtained from the seven natural growth areas in Turkey. The samples were cut with a circular saw, planed with a thickness machine, and sanded with a sanding machine ( with No. 80 sandpaper). After the samples were processed as radial and tangential surfaces in the machines, their surface roughness values ( R-a, R-y, and R-z) were measured in accordance with ISO 4288 ( 1996). According to the statistical results, the lowest surface roughness values were in the samples obtained from the Mugla and Samsun areas on the tangential surfaces that were processed with the thickness machine.Öğe The Effect of CYP1A1 and GSTP1 Isozymes on the Occurrence of Aortic Aneurysms(Georg Thieme Verlag Kg, 2015) Simsek, Erdal; Kilic, Murat; Simsek, Gulcin; Oguztuzun, Serpil; Moran, Busra; Saritas, Ahmet; Ulus, A. TulgaBackground Aortic aneurysms are vascular diseases that are associated with high mortality and morbidity. Cytochrome P450 CYP1A1 and glutathione S-transferase (GSTP1) isozymes were searched and compared with the patients who had experienced aortic surgery due to aortic aneurysm and atherosclerotic patients without aneurysm to find the relation of the oxidative stress with the aneurysms. Materials and Methods Study group consisted of the patients with the diagnosis of aortic aneurysm (group I, n: 12) and control group who were operated for coronary bypass surgery: preoperatively drug users (group II, n: 21) and nonusers (group Ill, n: 15). Paraffin sections (4 pm thick) of aortic biopsy materials were stained with hematoxylin and eosine, CYP1A1 and GSTP1 immunohistochemical markers. The specimens were evaluated using light microscopy at 40- to 400-fold magnification. Results The expressions of CYP1A1 and GSTP1 isozymes were found statistically significantly higher in the patients who have an aortic aneurysm than both the control groups (p < 0.05). There was no significant association between protein expressions, drugs and duration of usage, patient's demographic variables, and smoking (p > 0.05). Conclusions In this pioneering study, CYP1A1 and GSTP1 isozymes are related with the aneurysms. The strategy that prevents the oxidative stress for the patients who had aortic aneurysms could be a valuable choice of searching to effect the aneurysmal progression.Öğe Effect on Shear Strength of Machining Methods in Pinus nigra Arnold Bonded with Polyurethane and Polyvinyl Acetate Adhesives(North Carolina State Univ Dept Wood & Paper Sci, 2016) Kilic, MuratSpecimens taken from Pinus nigra Arnold were subject to surfacing techniques by being cut with a circular saw, planed with a thickness machine, and sanded with a calibrating sanding machine (with P80 grit sandpaper). First, their surface roughness values were measured; then, the specimens were processed in the machines in a radial and tangential process. Afterwards, the change in shear strength (adhesiveness resistance) was analyzed as a result of bonding with various adhesive types (PVAc, PU) and pressure applications (0.45 N/mm(2) or 0.9 N/mm(2)). Approximately 600 specimens were prepared with the purpose of identifying the effect of variables on the bonding performance, and they were subjected to shear testing. The greatest shear strength achieved for both the tangential and radial surfaces in terms of cutting was observed in specimens processed in the thickness machine, on which polyvinyl acetate adhesive and 0.9 N/mm(2). pressure were applied. Specimens bonded with polyvinyl acetate adhesive displayed higher shear strength in general in comparison to those bonded with polyurethane for both tangential and radial surfaces.Öğe Expression Levels of Glutathione S-Transferase and Cytochrome P450 Isoenzymes in Nasal Polyp Tissue(Springer India, 2023) Demir, Mehmet Goekhan; Aydin, Sedat; Oguztuezuen, Serpil; Basak, Kayhan; Bozer, Buesra M.; Kilic, MuratNasal polyps are benign sinonasal masses composed of eosinophils and extracellular edema. Pathogenesis of the polyp formation is unclear but several studies strongly suggest a correlation with infection, inflammation and allergy conditions. Our aim is to investigate the potential link between allergy and nasal polyp in tissue level. Nasal polyp group included 60 patients whose diagnosis was confirmed with biopsy and the control group included 38 healthy patients. Tissue sample of the control group was taken from inferior turbinate mucosa under local anesthesia and nasal polyp tissue was collected from functional endoscopic sinus surgery. The glutathione S-transferase (GST) and cytochrome P450 (CYP) isoenzyme expressions of the tissue samples were investigated under light microscopy and graded by a senior pathologist. GSTP1 protein expression was significantly higher in tissue samples from nasal polyp group compared to that of control group (p < 0.05). However, CYP1A1, GSTM1 and GSTA1 isoenzymes were not different between the two groups (p > 0.05). We have found that GSTP1 isoenzyme was elevated in nasal polyp tissue compared to the control. The increase in protein expression of GSTP1 might have occured as a tissue response to the increased oxidative stress thus suggesting a role of GSTP1 in polyp formation.Öğe Expression of antimicrobial peptides in recurrent adenotonsillitis(Walter De Gruyter Gmbh, 2016) Demir, Mehmet Gokhan; Aydin, Sedat; Erdogan, Banu Atalay; Oguztuzun, Serpil; Kilic, Murat; Barisik, Nagehan OzdemirBackground: Recurrent acute tonsillitis is one of the most frequent otorhinolaryngology clinic referrals, yet its pathogenesis remains poorly understood. Antimicrobial cationic peptides are components of the innate system. They are generally small, highly positively charged peptides with broad spectrum antimicrobial activity which function as the body's "natural antibiotics". Our aim is to investigate the role of antimicrobial cationic peptides in the susceptibility of patients to recurrent acute tonsillitis. Materials and methods: The study is done with 100 children who had a history of recurrent adenotonsillitis as subject group and 100 children with adenotonsillar hypertrophy as control group. Tonsillar and adenoid tissues are dissected into parts as deep and surface epithelium and investigated semiquantitatively with immunohistochemistry. Human beta defensin (hBD) 1-3 and cathelecidin (LL-37) levels are compared with microscopically. Results: Immunohistochemistry revealed a strong expression of hBD-1, hBD-2 and hBD-3 in tonsillar tissue. Quantification of hBD-1, hBD-2 and hBD-3 expressions are shown more in tonsillar tissue than in adenoids. LL-37 is one of the antimicrobial peptides found in human tonsillar tissue and adenoids, that participates in the innate immune system of these tissues. Statistically, hBD-1, hBD-3 and LL-37 expressions were different in recurrent tonsillitis tissue than control (p < 0.05). Moreover hBD-2 expression was different in adenoid tissue than control (p < 0.05). Conclusion: Antimicrobial peptides have key role in adenotonsillar infections and this defense mechanism increases susceptibility to recurrent infections in upper respiratory tract.Öğe The expression of GST and CYP isoenzymes in thyroid nodular hyperplasia and papillary thyroid cancer tissue: Correlation with clinical parameters(Entomology & Applied Science Research Letters-Easletters, 2016) Oguztuzun, Serpil; Ergn, Duygu; Kilic, Murat; Bozer, Busra; Simsek, Gulcin Guler; Bulus, HakanThis study investigated the immunohistochemical staining characteristics of glutathione-S-transferase (GST) pi(P), mu(M), theta(T), omega(O) and kappa(K) cytochrome P450 (CYP) A1, B1 and 2E1 isoenzymes in thyroid nodular hyperplasia (NH) and papillary thyroid cancer (PTC) tissues. For immunohistochemical studies, tissues from 18 patients with thyroid nodular hyperplasia, 28 patients with papillary thyroid cancer at the Kecioren Training and Research Hospital, Ankara, Turkey, were used. Relationships between GST and CYP isoenzyme expressions in NH and PTC tissues were examined by the Mann-Whitney U test, and clinicopathological data were examined by the Pearson Correlation Test and Regression Analysis. When the NH and PTC tissues from these cases were compared with respect to their staining intensity, GSTP1, GSTO1, GSTK1, CYP1A1, CYP2E1 expressions in PTC cells were significantly higher than those in NH epithelial cells (p< 0.05). There were no statistically significant differences in the CYP1B1, GSTT1 and GSTM1 expressions between benign and tumor epithelium (p> 0.05). There were significant association between GSTO1, GSTK1 expressions and sT3 levels in PTC (p< 0.05) and CYP1B1 expression in NH. There was a significant association between GSTO1 expression and smoking status in NH ( p< 0.05). There was no statistical relationship between the GSTM1, GSTT1, GSTP1, GSTO1, GSTK1, CYP1A1, CYP1B1, CYP2E1 isoenzyme expressions and the clinicopathological data (age, TSH, sT4 levels, tumor stage) ( p> 0.05). GSTP1, GSTO1, GSTK1, CYP1A1 and CYP2E1 isoenzymes may have roles in the carcinogenesis of the papillary thyroid cancer.Öğe The expression of GST isoenzymes and p53 in non-small cell lung cancer(Via Medica, 2010) Oguztuzun, Serpil; Aydin, Mehtap; Demirag, Funda; Yazici, Ulku; Ozhavzali, Muzeyyen; Kilic, Murat; Iscan, MesudeThis study investigated the immunohistochemical staining characteristics of glutathione-S-transferase alpha, pi, mu, theta and p53 in non-small cell lung carcinoma and normal lung tissue from 50 patients. The relationships between expressions of the Glutathione-S-transferase isoenzymes and some clinicopathological features were also examined. Expression of glutathione-S-transferase pi, mu, alpha, theta and p53 was assessed by immunohistochemistry for primary lung carcinomas of 50 patients from the Sanitarium Education and Research Hospital, Ankara lung cancer collection. The relationships between expression of the glutathione-S-transferase isoenzymes, p53 in normal and tumor tissue by Student T test and the clinicopathological data were also examined by Spearman Rank tests. When the normal and tumor tissue of these cases were compared according to their staining intensity and percentage of positive staining, glutathione-S-transferase alpha, pi, mu, theta expressions in tumor cells was significantly higher than normal cells (p<0.05). There was no significant difference in the expression of p53 between normal and tumor cells (p>0.05). When the immunohistochemical results of glutathione-S-transferase isoenzymes and p53 were correlated with the clinical parameters, there were no significant associations between glutathione-S-transferases and p53 expressions and tumor stage, tumor grade and smoking status (p>0.05).Öğe The expression of GST isoenzymes in acinar adenocarcinoma, intraepithelial neoplasia, and benign prostate tissue: correlation of clinical parameters with GST isoenzymes(Tubitak Scientific & Technical Research Council Turkey, 2012) Simsek, Gulcin; Oguztuzun, Serpil; Guresci, Servet; Kilic, Murat; Bozkurt, Omer Faruk; Unsal, AliThis study investigated the immunohistochemical staining characteristics of glutathione-S-transferase (GST) alpha, pi, mu, and theta in prostatic acinar adenocarcinoma (PCA), prostatic intraepithelial neoplasia (PIN), and benign prostatic tissues from 19 patients. Relationships between GST isoenzyme expression in benign, PIN, and PCA tissue were examined by the Wilcoxon signed-rank test and clinicopathological data were examined by the Spearman correlation rank test. When the benign, PIN, and PCA tissues from these cases were compared according to their staining intensity, GST alpha, pi, mu, and theta expressions in tumor cells were significantly lower than in benign epithelial cells (P<0.05). The GST alpha class displayed the lowest level of expression in PIN and PCA. Expression of GST pi was lower in PCA tissue than in PIN and benign epithelial tissue (P<0.05). We hypothesize that carcinogenesis in the prostate results from impaired cellular handling of mutagenic agents owing to reduction or loss of expression of multiple GST isoenzymes and other detoxifying and antimutagenesis agents. This study confirms the down-regulation of GST isoenzymes in PCA of the prostate and shows that the loss of GST isoenzyme expression is a phenotype associated with malignant transformation. There was no statistical relationship between GST isoenzyme expression and the clinicopathological data (age, Gleason score, and total serum prostate-specific antigen levels) (P>0.05).Öğe Expressions of CYP and GST Isoenzymes in Human Gastric Tumor and Non-Tumor Tissues(Akad Doktorlar Yayinevi, 2018) Simsek, Gulcin G.; Oguztuzun, Serpil; Bozer, Busra; Kilic, Murat; Kocdogan, Arzu K.; Kaygin, Pinar; Bulus, HakanIn this study we investigated the immunohistochemical staining characteristics of cytochrome P450 1A1 (CYP1A1), CYPB1, CYP2E1 and glutathione S-transferase P1 (GSTP1), GSTT1, GSTO1, GSTK1 isoenzymes in gastric tumor and surrounding tumor free (normal) gastric tissues from 40 patients. For immunohistochemical studies, tissues were obtained from 40 patients with gastric adenocarcinoma. Tumor and non-tumoral control tissues of patients were compared according to their staining intensity. Relationships between CYP and GST isoenzyme expressions in adenocarcinoma tissues were examined by the Mann Whitney-U test, and the clinicopathological data were examined by the Spearman's Rank Correlation test. CYP1B1, GSTT1, GSTO1 and GSTK1 expressions in gastric cancer cells were significantly higher than those in gastric normal epithelial cells (p < 0.05). However, CYP1A1, CYP2E1 and GSTP1 expressions were not significantly higher in tumor epithelium than those in normal epithelium in human gastric adenocancer (p < 0.05). Among the studied CYPs and GSTs, there was not statistically significant association between the studied isoenzyme expressions and age, gender and tumor grade ( p > 0.05). In patients with gastric adenocarcinoma, CYP1B1, GSTO1, GSTT1, and GSTK1 protein expressions are higher in tumor than normal gastric tissues.Öğe Expressions of glutathione S-transferase alpha, mu, pi, and theta in the skin samples of patients with acne rosacea(WILEY, 2020) Takci, Zennure; Bilgili, Serap Gunes; Kilic, Murat; Oguztuzun, Serpil; Bozer, Busra; Simsek, Gulcin Guler; Akbayrak, AtiyeBackground Data point to the importance of oxidative stress in rosacea. Glutathione S-transferases (GSTs) have substantial roles in a wide variety of oxidative stress-related conditions. Aim To evaluate the immunohistochemical staining characteristics of GST alpha (GSTA), mu (GSTM), pi (GSTP), and theta (GSTT) in patients with rosacea. Patients/Methods The study included 23 women and 7 men with rosacea (mean +/- SD age 49 +/- 11 year) and 15 healthy control subjects (10 women, 5 men; mean +/- SD age 47.86 +/- 10.88 year). For each patient, the average disease duration, disease subtype, ocular involvement, and severity score were recorded. A 3-mm punch biopsy was taken from the facial skin of each patient and control. Expression of GST isoenzymes was analyzed immunohistochemically. Results Expressions of GSTM1, GSTP1, and GSTT1 were significantly elevated in patients with rosacea compared to those in the control group (P = .0001,P = .0002,P < .0001, respectively). In the rosacea group, GSTT1 expression was significantly stronger than GSTP1 and GSTA1 expressions (P = .019,P < .0001, respectively). There were no significant associations between expressions of GST isoenzymes and gender, age, average duration of illness, disease subtype, ocular involvement, or severity score in the patient group (allP > .05). Conclusions In rosacea, the significant increase of GSTT1, GSTP1, and GSTM1 expressions might result from activation of GST as an outcome of extreme free radical generation from triggered neutrophils or ultraviolet vulnerability. These findings support the relevance of oxidant stress in the pathogenesis of rosacea.Öğe Glutathione S-transferase enzyme activity and protein expression in patients with recurrent tonsillitis and idiopathic tonsillar hypertrophy(Palacky Univ, Medical Fac, 2019) Aydin, Sedat; Demir, Mehmet Gokhan; Oguztuzun, Serpil; Kilic, Murat; Yilmaz, Can; Dirican, OnurObjectives. The palatine tonsil is a significant part of the secondary immune system. Tonsillitis and idiopathic tonsillar hypertrophy (ITH) are the most common pathologies of this component. Although there are studies on their pathogenesis, there is insufficient study of the role of antioxidant agents. Glutathione S-transferase (GST) isozymes contribute to the antioxidation reactions in the tissue via the glutathione pathway. The purpose in this study was to reveal the levels of the GST enzyme activity and protein expression of GSTP1 and GSTA1 isozymes in patients with tonsillitis and tonsil hypertrophy, and to investigate their role in the pathogenesis of these diseases. Materials and Methods. Sixteen patients with recurrent tonsillitis and 5 patients with ITH and were included in the study. Cytosolic extracts were prepared from post-tonsillectomy tissues of both patient groups and GST enzyme activities were measured. Results. The expression of GSTP1 was found to be significantly higher than GSTA1 in tissue samples of patients with ITH and recurrent tonsillitis (P<0.001). Increased GST activity and GSTP1 isozyme expression were shown in patients with recurrent tonsillitis compared to the idiopathic tonsillar hypertrophy study group.There was a positive correlation between the expressions of GSTP1 (P=0.040; r=0.47). Conclusion. Increased GST activity and GSTP1 isozymes were demonstrated histologically in the pathogenesis of ITH and recurrent tonsillitis. We believe that the data of changes in antioxidant capacity, obtained from studies with more extensive and larger samples, would support our findings.Öğe Immunohistochemical Expressions of the Antimicrobial Peptides (hBD-3 and hCAP-18/LL-37) in Colon, Stomach and Lung Adenocarcinomas(Akad Doktorlar Yayinevi, 2015) Kilic, Murat; Oguztuzun, Serpil; Simsek, Gulcin; Cakir, EbruThis study investigated the immunohistochemical staining characteristics of human beta defensin-3 (hBD-3) and human cationic antimicrobial peptide-18/cathelicidin (hCAP-18/LL-37) in colon, stomach and lung adenocarcinomas and normal tissues (periphery to tumor tissues) from 22, 24 and 24 patients, respectively. Expressions of hBD-3 and hCAP-18/LL-37 were assessed by immunohistochemistry for colon, stomach and lung adenocarcinomas of 70 patients from Ataturk Chest Diseases and Thoracic Surgery Training and Research Hospital and Kecioren Training and Research Hospital. both located in Ankara, Turkey. The differences between the expressions of hBD-3 and hCAP-18/LL-37 in normal and carcinoma tissues were analyzed by Mann-Whitney U Test. When the normal and tumor tissues of these cases were compared according to their staining intensity of positive staining, the hBD-3 and hCAP-18/LL-37 expressions in colon, stomach and lung adenocarcinomas cells were significantly higher than those in normal cells (p<0.05). Immunostaining of HBD-3 and hCAP-18/LL-37 was found to be a marker of malignancy in colon, stomach and lung adenocarcinomas. The expressions of hBD-3 and hCAP-18/LL-37 were, for the first time, shown to be significantly altered in colon, stomach and lung adenocarcinomas as compared to controls. In conclusion, the present findings suggest that beside the antimicrobial activity of Antimicrobial Peptides (AMPs), hBD-3 and hCAP-18/LL-37 can also play a role in the pathogenesis of colon, stomach and lung adenocarcinomas.Öğe Measuring The Surface Roughness Values Of European Hop-Horn Beam (Ostrya Carpinifolla Scop.) Wood(Slovak Forest Products Research Inst, 2018) Kilic, Murat; Purlusoy, Inci; Kurnali, MelihIn this study, the samples obtained from European hop-horn beam (Ostrya carpinifolia Scop.) wood has been subject to cutting with circular saw, planing with a thickness machine and sanding with a caliber sanding machine (with no: 80 sand). After the specimens were processed in the machines in radial and tangential surfaces, their surface roughness values (R-a, R-y, R-z) have been determined in accordance with the ISO 4288 standard. According to the statistical results, the lowest roughness values have been achieved with the thickness machine. Similarly, the roughness values of tangentially cut surfaces have been found to be lower than the radially cut surfaces.Öğe Polymorphisms and Protein Expressions of Glutathione S-Transferase M1 and T1 in Non-Small Cell Lung Cancer(Turkish Pharmacists Assoc, 2017) Kilic, Murat; Ada, Ahmet Oguz; Oguztuzun, Serpil; Demirag, Funda; Celik, Sezgin; Bicakcioglu, Pinar; Iscan, MumtazObjectives: The deletion polymorphisms of glutathione S-transferase (GST) GSTM1 and GSTT1 genes result in the absence of the corresponding protein, which decreases the detoxification of carcinogens. Studies evaluating polymorphisms and protein expressions in the same patients are limited. Therefore, in this study, we aimed to investigate the association between polymorphisms and protein expressions of GSTM1 and GSTT1 in lung tissues of patients with non-small cell lung cancer (NSCLC). Materials and Methods: For protein expression and gene deletion studies, tumor and surrounding tumor free (normal) tissue of 33 patients with NSCLC were used. In paraffin-embedded tissues, immunohistochemistry was used to detect protein expressions, and multiplex polymerase chain reaction amplification was used to identify gene deletions. Results: GSTM1 and GSTT1 protein expressions were not detected in patients with GSTM1 and GSTT1 gene deletions, whereas protein expressions were detected in lung tissues of all patients carrying GSTM1 and GSTT1 genes. The protein expression level of GSTT1 was 2.0-fold higher in tumors of patients lacking GSTM1 genes than those with GSTM1 genes (p=0.018). Protein expression of GSTM1 was statistically higher in tumor tissues than in normal tissues of patients with GSTM1 genes (p=0.001). Conclusion: These results show that a) there is an association between gene deletions and protein expressions of GSTM1 and GSTT1 in patients with NSCLC, b) in the absence of GSTM1 genes, enhancement of expression of GSTT1 in tumors is likely to show that GSTT1 increases its capacity to detoxify the toxic electrophiles in tumors, and c) GSTM1 protein expression is higher in tumors compared with normal lung tissues of patients with NSCLC.Öğe Usefulness of CA 15-3 for breast or ovarian primary sites in metastatic adenocarcinoma of pleural fluid(Tubitak Scientific & Technical Research Council Turkey, 2012) Oguztuzun, Serpil; Kilic, Murat; Tandogan, Nisa; Ozturk, LatifThis study was conducted to evaluate the diagnostic value of CA 15-3, mammaglobin, c-erbB-2, estrogen receptor (ER), progesterone receptor (PR), CK 20, and CA 125 for detecting metastatic breast and ovarian carcinoma in pleural fluids. The material under study consisted of 26 pleural effusions from invasive breast and ovarian cancer patients from the Hannover Cytopathology Institute. Expressions of these markers were studied using immunocytochemistry. All of the breast cancer cases studied showed a positive reaction with CA 15-3, while only 36% with mammaglobin, 21% with c-erbB-2, 36% with ER, and 7% with PR were positive. The sensitivities of CK 20, CA 15-3, and CA 125 as markers for metastatic ovarian carcinoma were 43%, 88%, and 55%, respectively. Our results indicate that CA 15-3 has a higher sensitivity than other tumor markers for diagnosing metastatic breast and ovarian carcinoma in pleural fluids.
