RELATIONSHIP BETWEEN TOXICITY OF CRYOPROTECTANTS, OSMOTIC AND OXIDATIVE STRESSES IN AWASSI RAM SPERM

Basit Öğe Kaydı
dc.contributor.authorVarisli, Omer
dc.contributor.authorBozkaya, Faruk
dc.contributor.authorAydilek, Nurettin
dc.contributor.authorTaskin, Abdullah
dc.date.accessioned2025-01-21T16:44:00Z
dc.date.available2025-01-21T16:44:00Z
dc.date.issued2022
dc.departmentKırıkkale Üniversitesi
dc.description.abstractBACKGROUND: The relationship between the toxicity of cryoprotectants and their osmotic and/or oxidative stresses remains to be further investigated. OBJECTIVE: To investigate the toxic effects of different cryoprotectants and osmotic stress on Awassi ram sperm and to determine the relationship between oxidative and antioxidative status of the sperm. MATERIALS AND METHODS: Pooled sperm samples were exposed to sucrose solutions of different concentrations (75 to 900 mOsm) and isosmotic condition (290-325 mOsm) was re-established by adding HEPES buffered Tyrode's lactate. Sperm samples were mixed with 0.5, 1.0 and 1.5 M of glycerol, methanol, 2-methoxyethanol, dimethylacetamide or 1,2-propanediol for 5 min and returned to isosmotic condition. Sperm samples were exposed to cryoprotectants at 4 degrees C for 2 hours and isosmotic conditions were re-established. Motility, viability, acrosome integrity and oxidative or antioxidative parameters were determined. RESULTS: Treatment with hypo- or hyperosmotic sucrose solution reduced motility and viability without affecting acrosome integrity. The addition and removal of glycerol and dimethylacetamide (1.0 or 1.5 M) decreased sperm motility, while cryoprotectants had no effect on viability except for 1.5 M glycerol. Chilling significantly reduced the motility and viability of the sperm, but not the acrosome integrity. Rapid addition or removal of cryoprotectants also did not affect the acrosome integrity. Cryoprotectants changed only the ceruloplasmin level, while there were significant post-chilling differences in lipid hydroperoxide, paraoxonase and ceruloplasmin levels. CONCLUSION: Cryoprotectants without other additives have limited protection and glycerol can be toxic to spermatozoa. The oxidative stress plays a role in cryoprotectant toxicity and chilling stress.
dc.description.sponsorshipScientific and Technological Research Council of Turkey [112O911]
dc.description.sponsorshipThe work was supported by the Scientific and Technological Research Council of Turkey (Grant #112O911).
dc.identifier.doi10.54680/fr22210110612
dc.identifier.endpage128
dc.identifier.issn0143-2044
dc.identifier.issn1742-0644
dc.identifier.issue2
dc.identifier.pmid36626153
dc.identifier.scopus2-s2.0-85146133707
dc.identifier.scopusqualityQ3
dc.identifier.startpage120
dc.identifier.urihttps://doi.org/10.54680/fr22210110612
dc.identifier.urihttps://hdl.handle.net/20.500.12587/25377
dc.identifier.volume43
dc.identifier.wosWOS:000802250600007
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherCryo Letters
dc.relation.ispartofCryoletters
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectcryoprotectants; oxidative stress; ram; toxicity; sperm
dc.titleRELATIONSHIP BETWEEN TOXICITY OF CRYOPROTECTANTS, OSMOTIC AND OXIDATIVE STRESSES IN AWASSI RAM SPERM
dc.typeArticle

Dosyalar

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu