Extracellular Trap Formation by Donkey Polymorphonuclear Neutrophils Against Toxoplasma gondii

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dc.contributor.authorYildiz, Kader
dc.contributor.authorGokpinar, Sami
dc.contributor.authorSursal, Neslihan
dc.contributor.authorBabur, Cahit
dc.contributor.authorOzen, Dogukan
dc.contributor.authorAzkur, Ahmet Kursat
dc.date.accessioned2020-06-25T18:34:21Z
dc.date.available2020-06-25T18:34:21Z
dc.date.issued2019
dc.departmentKırıkkale Üniversitesi
dc.descriptionSursal, Neslihan/0000-0002-4144-9520; Ozen, Dogukan/0000-0003-1943-2690; YILDIZ, Kader/0000-0001-5802-6156;
dc.description.abstractToxoplasma (T.) gondii is an obligatory intracellular apicomplexan parasite. The donkey is one of the intermediate hosts of T. gondii. There are almost no data about T. gondii infection in donkeys, apart from a few seroprevalence reports. The aim of the study was to detect the formation of extracellular traps by donkey polymorphonuclear neutrophils (PMNs) when exposed to T. gondii tachyzoites in vitro. Tachyzoites were observed to be entrapped within extracellular traps (NET) structures. Myeloperoxidase, neutrophil elastase (NE), and histone (H3) were observed in NET structures. NE and reactive oxygen species activity increased with time and was higher in the PMN-tachyzoite cocultures. Some tachyzoites were seen close localization to the nucleus of neutrophils. Degranulation and extracellular traps were observed simultaneously in some donkey neutrophils after incubation for 1 hour. The tachyzoite invasion rate decreased in PMN-tachyzoite cocultures in comparison to the controls. NETs can negatively affect the viability of entrapped tachyzoites in addition to their action of physical entrapment. NE may play a key role in the viability of T. gondii in donkeys. The amount of extracellular DNA increased with incubation time in the PMN-tachyzoite cocultures (P < .001). This is a first report regarding the formation of donkeys NETs after exposure to T. gondii tachyzoites in vitro. Unlike sheep and cattle PMN, degranulation and extracellular traps developed in the same neutrophil. Further studies focusing on signaling pathways may shed light on what determines the behavior of donkey neutrophils after exposure to T. gondii. (C) 2018 Elsevier Inc. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.jevs.2018.11.002
dc.identifier.endpage9en_US
dc.identifier.issn0737-0806
dc.identifier.issn1542-7412
dc.identifier.scopus2-s2.0-85057528269
dc.identifier.scopusqualityQ1
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1016/j.jevs.2018.11.002
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7878
dc.identifier.volume73en_US
dc.identifier.wosWOS:000456711400001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Incen_US
dc.relation.ispartofJournal Of Equine Veterinary Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectToxoplasma gondiien_US
dc.subjectDonkeyen_US
dc.subjectNeutrophil extracellular trapsen_US
dc.subjectInnate immunityen_US
dc.subjectIn vitroen_US
dc.titleExtracellular Trap Formation by Donkey Polymorphonuclear Neutrophils Against Toxoplasma gondiien_US
dc.typeArticle

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