Polyethyleneimine brushes effectively inhibit encrustation on polyurethane ureteral stents both in dynamic bioreactor and in vivo

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dc.contributor.authorGultekinoglu, Merve
dc.contributor.authorKurum, Baris
dc.contributor.authorKarahan, Siyami
dc.contributor.authorKart, Didem
dc.contributor.authorSagiroglu, Meral
dc.contributor.authorErtas, Nusret
dc.contributor.authorUlubayram, Kezban
dc.date.accessioned2020-06-25T18:23:13Z
dc.date.available2020-06-25T18:23:13Z
dc.date.issued2017
dc.departmentKırıkkale Üniversitesi
dc.descriptionERTAS, NUSRET/0000-0002-9770-3292
dc.description.abstractPolyurethane (PU) ureteral stents have been widely used as biomedical devices to aid the flow of the urine. Due to the biofilm formation and encrustation complications it has been hindered their long term clinical usage. To overcome these complications, in this study, cationic polyethyleneimine (PEI) brushes grafted on PU stents and their performances were tested both in a dynamic biofilm reactor system (in vitro) and in a rat model (in vivo). Thus, we hypothesized that PEI brushes inhibit bacterial adhesion owing to the dynamic motion of brushes in liquid environment. In addition, cationic structure of PEI disrupts the membrane and so kills the bacteria on time of contact. Cationic PEI brushes decreased the biofilm formation up to 2 orders of magnitude and approximately 50% of encrustation amount in respect to unmodified PU, in vitro. In addition, according to Atomic Absorption Spectroscopy (AAS) results, approximately 90% of encrustation was inhibited on in vivo animal models. Decrease in encrustation was clearly observed on the stents obtained from rat model, by Scanning Electron Microscopy (SEM). Also, histological evaluations showed that; PEI brush grafting decreased host tissue inflammation in close relation to decrease in biofilm formation and encrustation. As a results; dual effect of anti adhesive and contact-killing antibacterial strategy showed high efficiency on PEI brushes grafted PU stents both in vitro and in vivo. (C) 2016 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipScientific and Technological Research Council Turkey of (TUBITAK) [112M293]en_US
dc.description.sponsorshipThis study is financially supported by The Scientific and Technological Research Council Turkey of (TUBITAK) (Grant no: 112M293).en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.msec.2016.11.125
dc.identifier.endpage1174en_US
dc.identifier.issn0928-4931
dc.identifier.issn1873-0191
dc.identifier.pmid27987673
dc.identifier.scopus2-s2.0-85006127316
dc.identifier.scopusqualityN/A
dc.identifier.startpage1166en_US
dc.identifier.urihttps://doi.org/10.1016/j.msec.2016.11.125
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7046
dc.identifier.volume71en_US
dc.identifier.wosWOS:000390967200135
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bven_US
dc.relation.ispartofMaterials Science & Engineering C-Materials For Biological Applications
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAntibacterial brushesen_US
dc.subjectPolyethyleneimineen_US
dc.subjectUreteralen_US
dc.subjectstenten_US
dc.subjectBiofilm Encrustationen_US
dc.titlePolyethyleneimine brushes effectively inhibit encrustation on polyurethane ureteral stents both in dynamic bioreactor and in vivoen_US
dc.typeArticle

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