Synthesis, in-vitro cytotoxic activity and DNA interactions of new dicarboxylatoplatinum(II) complexes with 2-hydroxymethylbenzimidazole as carrier ligands

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dc.contributor.authorUtku, Semra
dc.contributor.authorOzcelik, Azime Berna
dc.contributor.authorGumus, Fatma
dc.contributor.authorYilmaz, Sukran
dc.contributor.authorArsoy, Taibe
dc.contributor.authorAcik, Leyla
dc.contributor.authorKeskin, Ayten Celebi
dc.date.accessioned2020-06-25T18:08:03Z
dc.date.available2020-06-25T18:08:03Z
dc.date.issued2014
dc.departmentKırıkkale Üniversitesi
dc.descriptionUTKU, Semra/0000-0003-3181-9134
dc.description.abstractObjectivesThe aim of this study was to investigate the in-vitro cytotoxic activity of new platinum(II) complexes on the human HeLa (ER-), MCF-7 (ER+) and MDA-MB 231 (ER-) cell lines. Furthermore, we investigated plasmid DNA interactions and inhibition of BamHI and HindIII restriction enzyme activity of the complex 1-4,7. MethodsPlatinum(II) complexes were synthesised from precursor complexes of [PtL2Cl2] and [PtL2I2]. Their cytotoxic activity was tested by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Their plasmid DNA interactions and restriction enzyme activities were also investigated using the agarose gel electrophoresis method. Key findingsThe growth inhibitory effect results showed that the cytotoxicity of complex 2 was found to be the most active complex among the synthesised complexes. ConclusionsThe MTT results showed that complex 2 was found to be cytotoxic equal to cisplatin and higher than carboplatin against the MCF-7 and MDA-MB-231 cell lines. Furthermore, the estrogen or progesterone co-treatment slightly increased the cytotoxicity of complex 2, the cisplatin and carboplatin compared with the complex 2 tested alone in 50m concentration. According to plasmid DNA interaction and the restriction studies, complexes 1-4,7 modified the tertiary structure of pBR322 plasmid DNA, and complexes 2-4 prevented enzyme digestion at high concentrations.en_US
dc.description.sponsorshipResearch Foundation of Gazi UniversityGazi University [EF 02/2007-24]en_US
dc.description.sponsorshipThis work was supported by Research Foundation of Gazi University (EF 02/2007-24). The authors are grateful to Dr Bahar Tasdelen (Mersin University, Faculty of Medicine Department of Biostatistics and Medical Informatics) for helpful statistical analysis support.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1111/jphp.12290
dc.identifier.endpage1605en_US
dc.identifier.issn0022-3573
dc.identifier.issn2042-7158
dc.identifier.issue11en_US
dc.identifier.pmid25109360
dc.identifier.scopus2-s2.0-84915803821
dc.identifier.scopusqualityQ2
dc.identifier.startpage1593en_US
dc.identifier.urihttps://doi.org/10.1111/jphp.12290
dc.identifier.urihttps://hdl.handle.net/20.500.12587/5716
dc.identifier.volume66en_US
dc.identifier.wosWOS:000343922100009
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWileyen_US
dc.relation.ispartofJournal Of Pharmacy And Pharmacology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbenzimidazoleen_US
dc.subjectcytotoxic activityen_US
dc.subjectDNA bindingen_US
dc.subjectgel electrophoresisen_US
dc.subjectplatinum(II) complexesen_US
dc.titleSynthesis, in-vitro cytotoxic activity and DNA interactions of new dicarboxylatoplatinum(II) complexes with 2-hydroxymethylbenzimidazole as carrier ligandsen_US
dc.typeArticle

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