Affinity interaction of hydroxypyruvate reductase from Methylophilus spp. with Cibacron blue F3GA-derived poly(HEMA EGDMA) microspheres: partial purification and characterization

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dc.contributor.authorArica, MY
dc.contributor.authorHalicigil, C
dc.contributor.authorAlaeddinoglu, G
dc.contributor.authorDenizli, A
dc.date.accessioned2020-06-25T17:34:39Z
dc.date.available2020-06-25T17:34:39Z
dc.date.issued1999
dc.departmentKırıkkale Üniversitesi
dc.description.abstractA methylotrophic hydroxypyruvate reductase was partially purified and characterized from Methylophilus spp. using the biomimetic dye, Cibacron Blue F3FA attached to poly(HEMA-EGDMA) microspheres. The absorption capacities of the dye-affinity microspheres were determined by changing pH and the concentration of the proteins in the adsorption medium. Hydroxypyruvate reductase was desorbed from the dye-affinity support specifically with 2 mM NADH solution. The enzyme was purified 10.4-fold with 47% yield. The molecular mass and subunit molecular mass of the enzyme was estimated to be 75 kDa and 37 kDa on the basis of its mobility in polyacrylamide and SDS-polyacrylamide gels, respectively. This suggested a homogeneous dimer structure. The optimal pH was between 5.0 and 7.0, and the maximum enzyme activity was obtained at 50 degrees C. The K-m values of hydroxpyruvate reductase were 0.222 mM for hydroxpyruvate and 0.067 mM for NADH. (C) 1999 Elsevier Science Ltd. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.endpage381en_US
dc.identifier.issn1359-5113
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-0344224217
dc.identifier.scopusqualityQ1
dc.identifier.startpage375en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2823
dc.identifier.volume34en_US
dc.identifier.wosWOS:000081089900008
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofProcess Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjecthydroxypyruvate reductaseen_US
dc.subjectNADH dependent enzymeen_US
dc.subjectMethylophilus spp.en_US
dc.subjectaffinity adsorptionen_US
dc.subjectpoly(HEMA-EGDMA) microspheresen_US
dc.subjectCibacron Blue F3GAen_US
dc.titleAffinity interaction of hydroxypyruvate reductase from Methylophilus spp. with Cibacron blue F3GA-derived poly(HEMA EGDMA) microspheres: partial purification and characterizationen_US
dc.typeArticle

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