Alcohol determination via covalent enzyme immobilization on magnetic beads

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dc.contributor.authorKiralp, Senem
dc.contributor.authorTopcu, Asuman
dc.contributor.authorBayramoglu, Guelay
dc.contributor.authorArica, M. Yakup
dc.contributor.authorToppare, Levent
dc.date.accessioned2020-06-25T17:48:01Z
dc.date.available2020-06-25T17:48:01Z
dc.date.issued2008
dc.description.abstractAlcohol oxidase was covalently immobilized onto magnetic beads of two different sizes, 75-150 mu m and 50-75 mu m in diameter, fabricated in the presence of glycidylmethacrylate and methylmethacrylate via suspension polymerization in the presence of a cross-linker (i.e., ethylenedimethylmethacrylate). The activity of the enzyme on smaller microspheres was found to be almost 4.8 fold higher than that of the larger counterparts. Although enzyme loading was same for both fractions, activity and the affinity of immobilized enzyme were significantly altered. The effects of various parameters such as temperature, pH, operational and storage stability were examined. The substantial change in the activity of enzyme was also observed in stability experiments in favor of large size magnetic beads. For all stability experiments including storage stability, the 75-150 mu m fraction was found to be more sentinel support for the enzyme. (C) 2007 Elsevier B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.snb.2007.07.035
dc.identifier.endpage528en_US
dc.identifier.issn0925-4005
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-37549019657
dc.identifier.scopusqualityQ1
dc.identifier.startpage521en_US
dc.identifier.urihttps://doi.org10.1016/j.snb.2007.07.035
dc.identifier.urihttps://hdl.handle.net/20.500.12587/4243
dc.identifier.volume128en_US
dc.identifier.wosWOS:000252792100024
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Saen_US
dc.relation.ispartofSensors And Actuators B-Chemical
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectmagnetic beadsen_US
dc.subjectenzyme immobilizationen_US
dc.subjectalcohol oxidaseen_US
dc.subjectkinetic parametersen_US
dc.subjectstabilityen_US
dc.titleAlcohol determination via covalent enzyme immobilization on magnetic beadsen_US
dc.typeArticle

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