Effects of different extenders and additives on liquid storage of Awassi ram semen

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dc.contributor.authorVarisli, Omer
dc.contributor.authorTaskin, Abdullah
dc.contributor.authorAkyol, Numan
dc.date.accessioned2020-06-25T18:29:54Z
dc.date.available2020-06-25T18:29:54Z
dc.date.issued2018
dc.departmentKırıkkale Üniversitesi
dc.descriptionAKYOL, NUMAN/0000-0001-5959-3486; Varisli, Omer/0000-0002-2777-3586
dc.description.abstractThe aim of this study was to evaluate the effects of different extenders and additives on ram semen during liquid storage and to demonstrate the role of oxidative stress parameters on this process. In the present study, ejaculates taken by artificial vagina twice a week from 4 rams during the breeding season were used. They were mixed and used if motility and viability were above 70% and there was a 95% intact acrosome. The semen specimens were diluted by Tris-citrate-glucose (TRIS), Tris-TES (TEST), HEPES-buffered Tyrode lactate (TL-HEPES), and Dulbecco's phosphate-buffered saline (PBS) extenders supplemented with different additives [centrifuged egg yolk, Equex-STM, bovine serum albumin (BSA), and ethylenediaminetetraacetic acid (EDTA)]. The specimens were stored at 4 degrees C for up to 96 h and evaluated for motility, membrane integrity, acrosome integrity, mitochondrial membrane potential (MMP), and oxidative stress parameters. At the end of the 96-h storage, the highest sperm motility was 64.2 +/- 3.7% (P < 0.05) and significant loss of sperm motility and membrane integrity were not detected in extender TEST-3, but the MMP rate significantly declined. Acrosome integrity was not affected by storage time or extender types. BSA and EDTA decreased lipid peroxidation (LPO) and total oxidant status (TOS), but did not positively affect motility or membrane integrity. As a result, TRIS, TEST, and TE-FIEPES-3 were observed to provide better protection for ram semen during liquid storage at 4 degrees C than other extenders. The role of oxidative stress and MMP are considerable in liquid storage.en_US
dc.description.sponsorshipHUBAK (Harran University Scientific Research Council)Harran University [14007]en_US
dc.description.sponsorshipThe present work was supported by HUBAK (Harran University Scientific Research Council) grant number 14007. I would like to thank my fellow graduate students for their help.en_US
dc.identifier.citationVarışlı, Ömer; Taşkın, Abdullah; And Akyol, Numan (2018) "Effects of different extenders and additives on liquid storage of Awassi ram semen," Turkish Journal of Veterinary & Animal Sciences: Vol. 42: No. 4, Article 2.en_US
dc.identifier.doi10.3906/vet-1801-82
dc.identifier.endpage242en_US
dc.identifier.issn1300-0128
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85051664867
dc.identifier.scopusqualityQ3
dc.identifier.startpage230en_US
dc.identifier.trdizinid354976
dc.identifier.urihttps://doi.org/10.3906/vet-1801-82
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7496
dc.identifier.volume42en_US
dc.identifier.wosWOS:000447159000002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherScientific Technical Research Council Turkey-Tubitaken_US
dc.relation.ispartofTurkish Journal Of Veterinary & Animal Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAwassi ramen_US
dc.subjectextendersen_US
dc.subjectliquid storageen_US
dc.subjectsupplementsen_US
dc.subjectspermen_US
dc.titleEffects of different extenders and additives on liquid storage of Awassi ram semenen_US
dc.typeArticle

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