Monosize and non-porous p(HEMA-co-MMA) microparticles designed as dye- and metal-chelate affinity sorbents

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dc.contributor.authorDenizli, A
dc.contributor.authorYavuz, H
dc.contributor.authorArica, Y
dc.date.accessioned2020-06-25T17:34:40Z
dc.date.available2020-06-25T17:34:40Z
dc.date.issued2000
dc.departmentKırıkkale Üniversitesi
dc.description.abstractCongo red was immobilised onto monosize and non-porous poly(2-hydroxyethylmethacrylate-co-methylmethacrylate) [p(HEMA-co-MMA)] copolymer microparticles (4.0 mu m in diameter). Then Fe(III) ions were complexed by chelation with the immobilised congo red molecules. Different amounts of Fe(III) ions were loaded on the dye-derived microparticles by changing the concentration of Fe(III) ions and pH of the reaction medium. Congo red-derived and Fe(III)-complexed microparticles were used in the adsorption of glucose oxidase, catalase, lysozyme and bovine serum albumin. The maximum adsorption capacities of these microparticles were determined by changing pH and the concentration of the proteins in the adsorption medium. Their adsorption behavior can be described at least approximately with the Langmuir equation. Glucose oxidase, catalase, lysozyme and bovine serum albumin adsorption capacities of the Fe(III) complexed microparticles (165.1, 135.2, 67.6 and 44.5 mg g(-1)) were higher than those of the congo red-immobilised microparticles (125.9, 94.2, 35.8 and 21.2 mg g(-1), respectively). The non-specific adsorption of the proteins on the p(HEMA-co-MMA) microparticles was negligible. The resulting dye- and metal-chelate affinity microparticles have excellent reusability and long term storage stability. (C) 2000 Elsevier Science B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/S0927-7757(00)00485-4
dc.identifier.endpage317en_US
dc.identifier.issn0927-7757
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-0034580796
dc.identifier.scopusqualityQ1
dc.identifier.startpage307en_US
dc.identifier.urihttps://doi.org/10.1016/S0927-7757(00)00485-4
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2835
dc.identifier.volume174en_US
dc.identifier.wosWOS:000089588400003
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Bven_US
dc.relation.ispartofColloids And Surfaces A-Physicochemical And Engineering Aspects
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectglucose oxidaseen_US
dc.subjectcatalaseen_US
dc.subjectlysozymeen_US
dc.subjectbovine serum albuminen_US
dc.subjectp(HEMA-co-MMA)en_US
dc.subjectaffinity microspheresen_US
dc.subjectmetal chelate adsorptionen_US
dc.subjectcongo reden_US
dc.titleMonosize and non-porous p(HEMA-co-MMA) microparticles designed as dye- and metal-chelate affinity sorbentsen_US
dc.typeArticle

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