Raman spectroscopy and imaging of β-carotene in live corpus luteum cells

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dc.contributor.authorArikan, S.
dc.contributor.authorSands, H.S.
dc.contributor.authorRodway, R.G.
dc.contributor.authorBatchelder, D.N.
dc.date.accessioned2020-06-25T17:35:01Z
dc.date.available2020-06-25T17:35:01Z
dc.date.issued2002
dc.departmentKırıkkale Üniversitesi
dc.description.abstractRaman spectroscopy has been used to identify and locate beta-carotene within individual living luteal cells. The cells were either freshly prepared or cultured; the latter was incubated in the presence or absence of beta-carotene in the form of enriched bovine high-density lipoprotem. Luteal cells were investigated using several Raman spectroscopic and imaging techniques. These techniques did not give accurate concentration levels of beta-carotene within parts of the cell but illustrated the distribution of the molecule. Freshly prepared luteal cells were found to contain an appreciable concentration of beta-carotene. Over a period of several days, the concentration gradually reduced to a nearly undetectable level; similar results were found for cells cultured in the absence of the beta-carotene. For cells cultured in the presence of beta-carotene, the molecular concentration was maintained for as long as 2 weeks. The Raman spectra of fragmented cells showed that the beta-carotene is predominantly localised in the lipid-rich cell components, with the concentration highest in the microsomal fraction. The Raman imaging techniques revealed that beta-carotene was spread over the entire volume of the luteal cells with higher levels occurring at distinct sites, including the surface. (C) 2002 Elsevier Science B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/S0378-4320(02)00020-9
dc.identifier.endpage266en_US
dc.identifier.issn0378-4320
dc.identifier.issue3-4en_US
dc.identifier.pmid12047933
dc.identifier.scopus2-s2.0-0037124231
dc.identifier.scopusqualityQ1
dc.identifier.startpage249en_US
dc.identifier.urihttps://doi.org/10.1016/S0378-4320(02)00020-9
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2995
dc.identifier.volume71en_US
dc.identifier.wosWOS:000176451100010
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bven_US
dc.relation.ispartofAnimal Reproduction Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcattle-ovaryen_US
dc.subjectluteal cellsen_US
dc.subjectcaroteneen_US
dc.subjectRaman spectroscopyen_US
dc.titleRaman spectroscopy and imaging of β-carotene in live corpus luteum cellsen_US
dc.typeArticle

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