Dual delivery of platelet-derived growth factor and bone morphogenetic factor-6 on titanium surface to enhance the early period of implant osseointegration

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dc.contributor.authorKeceli, H. Gencay
dc.contributor.authorBayram, Cem
dc.contributor.authorCelik, Ekin
dc.contributor.authorErcan, Nuray
dc.contributor.authorDemirbilek, Murat
dc.contributor.authorNohutcu, Rahime Meral
dc.date.accessioned2021-01-14T18:10:26Z
dc.date.available2021-01-14T18:10:26Z
dc.date.issued2020
dc.departmentKKÜ
dc.descriptionBayram, Cem/0000-0001-8717-4668; Keceli, Huseyin Gencay/0000-0001-6695-2133
dc.description.abstractObjective To test the surface properties and in vitro effects of a new sequential release system on MC3T3-E1 cells for improved osseointegration. Background BMP6-loaded anodized titanium coated with PDGF containing silk fibroin (SF) may improve osseointegration. Methods Titanium surfaces were electrochemically anodized, and SF layer was covered via electrospinning. Five experimental groups (unanodized Ti (Ti), anodized Ti (AnTi), anodized + BMP6-loaded Ti (AnTi-BMP6), anodized + BMP6 loaded + silk fibroin-coated Ti (AnTi-BMP6-SF), and anodized + BMP6-loaded + silk fibroin with PDGF-coated Ti (AnTi-BMP6-PDGF-SF)) were tested. After SEM characterization, contact angle analysis, and FTIR analysis, the amount of released PDGF and BMP6 was detected using ELISA. Cell proliferation (XTT), mineralization, and gene expression (RUNX2andALPL) were also evaluated. Results After successful anodization and loading of PDGF and BMP6, contact angle measurements showed hydrophobicity for TiO(2)and hydrophilicity for protein-adsorbed surfaces. In FTIR, protein-containing surfaces exhibited amide-I, amide-II, and amide-III bands at 1600 cm(-1)-1700 cm(-1), 1520 cm(-1)-1540 cm(-1), and 1220 cm(-1)-1300 cm(-1)spectrum levels with a significant peak in BMP6- and/or SF-loaded groups at 1100 cm(-1). PDGF release and BMP6 release were delayed, and relatively slower release was detected in SF-coated surfaces. Higher MC3T3-E1 proliferation and mineralization and lower gene expression ofRUNX2andALPLwere detected in AnTi-BMP6-PDGF-SF toward day 28. Conclusion The new system revealed a high potential for an improved early osseointegration period by means of a better factor release curve and contribution to the osteoblastic cell proliferation, mineralization, and associated gene expression.en_US
dc.description.sponsorshipTurkiye Bilimsel ve Teknolojik Arastirma KurumuTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113S804]en_US
dc.description.sponsorshipTurkiye Bilimsel ve Teknolojik Arastirma Kurumu, Grant/Award Number: 113S804en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1111/jre.12756
dc.identifier.endpage704en_US
dc.identifier.issn0022-3484
dc.identifier.issn1600-0765
dc.identifier.issue5en_US
dc.identifier.pmid32776328
dc.identifier.scopus2-s2.0-85089144408
dc.identifier.scopusqualityQ1
dc.identifier.startpage694en_US
dc.identifier.urihttps://doi.org/10.1111/jre.12756
dc.identifier.urihttps://hdl.handle.net/20.500.12587/12603
dc.identifier.volume55en_US
dc.identifier.wosWOS:000557380800001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWILEYen_US
dc.relation.ispartofJOURNAL OF PERIODONTAL RESEARCH
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbone morphogenetic factor-6en_US
dc.subjectbone regenerationen_US
dc.subjectdental implanten_US
dc.subjectnanotechnologyen_US
dc.subjectnanotubesen_US
dc.subjectosseointegrationen_US
dc.subjectosteoblasten_US
dc.subjectplatelet-derived growth factoren_US
dc.subjectsequential releaseen_US
dc.subjectsilk fibroinen_US
dc.titleDual delivery of platelet-derived growth factor and bone morphogenetic factor-6 on titanium surface to enhance the early period of implant osseointegrationen_US
dc.typeArticle

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