DNA adsorption on a poly-L-lysine-immobilized poly(2-hydroxyethyl methacrylate) membrane

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dc.contributor.authorSenel, S.
dc.contributor.authorBayramoglu, G.
dc.contributor.authorArica, M.Y.
dc.date.accessioned2020-06-25T17:35:27Z
dc.date.available2020-06-25T17:35:27Z
dc.date.issued2003
dc.departmentKırıkkale Üniversitesi
dc.description.abstractThe DNA adsorption properties of poly-L-lysine-immobilized poly(2-hydroxyethyl methacrylate) (pHEMA) membrane were investigated. The pHEMA membrane was prepared by UV-initiated photopolymerization and activated with epichlorohydrin. Poly-L-lysine was then immobilized on the activated pHEMA membrane by covalent bonding, via a direct chemical reaction between the amino group of poly-L-lysine and the epoxy group of pHEMA. The poly-L-lysine content of the membrane was determined as 1537 mg m(-2). The poly-L-lysine-immobilized membrane was utilized as an adsorbent in DNA adsorption experiments. The maximum adsorption of DNA on the poly-L-lysine-immobilized pHEMA membrane was observed at 4 degreesC from phosphate-buffered salt solution (pH 7.4, 0.1 M; NaCl 0.5 M) containing different amounts of DNA. The non-specific adsorption of DNA on the plain pHEMA membrane was low (about 263 mg m(-2)). Higher DNA adsorption values (up to 5849 mg m(-2)) were obtained in which the poly-L-lysine-immobilized pHEMA membrane was used. (C) 2003 Society of Chemical Industry.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1002/pi.1229
dc.identifier.endpage1174en_US
dc.identifier.issn0959-8103
dc.identifier.issn1097-0126
dc.identifier.issue7en_US
dc.identifier.scopus2-s2.0-0038335386
dc.identifier.scopusqualityQ1
dc.identifier.startpage1169en_US
dc.identifier.urihttps://doi.org/10.1002/pi.1229
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3123
dc.identifier.volume52en_US
dc.identifier.wosWOS:000183923600018
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWileyen_US
dc.relation.ispartofPolymer International
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDNAen_US
dc.subjectpoly-L-lysineen_US
dc.subjectimmobilizationen_US
dc.subjectadsorptionen_US
dc.subjectaffinity membraneen_US
dc.subjectpoly(2-hydroxyethyl methacrylateen_US
dc.titleDNA adsorption on a poly-L-lysine-immobilized poly(2-hydroxyethyl methacrylate) membraneen_US
dc.typeArticle

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