Covalent immobilization of invertase on microporous pHEMA-GMA membrane

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dc.contributor.authorDanisman, T
dc.contributor.authorTan, S
dc.contributor.authorKacar, Y
dc.contributor.authorErgene, A
dc.date.accessioned2020-06-25T17:40:08Z
dc.date.available2020-06-25T17:40:08Z
dc.date.issued2004
dc.departmentKırıkkale Üniversitesi
dc.descriptionKacar, Yasemin/0000-0002-8682-9228
dc.description.abstractPoly (2-hydroxyethyl methacrylate-glycidyl methacrylate) (pHEMA-GMA) membrane was prepared by UV-initiated photopolymerization. Invertase was immobilized by the condensation reaction of the epoxy groups of glycidyl methacrylate in the membrane structure with amino groups of the enzyme. The (Km) values were 22 mM and 58 mM for free and immobilized enzyme, respectively. Immobilization improved the pH stability and temperature stability of the enzyme. Thermal stability was found to increase with immobilization. The half times for the activity decay at 70 degreesC were found to be 11 and 38 min for the free and immobilized enzyme, respectively. The immobilized enzyme activity was found to be quite stable in later experiments. (C) 2003 Elsevier Ltd. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.foodchem.2003.07.015
dc.identifier.endpage466en_US
dc.identifier.issn0308-8146
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-0346059510
dc.identifier.scopusqualityQ1
dc.identifier.startpage461en_US
dc.identifier.urihttps://doi.org/10.1016/j.foodchem.2003.07.015
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3300
dc.identifier.volume85en_US
dc.identifier.wosWOS:000188543800018
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofFood Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectpHEMA-GMA membraneen_US
dc.subjectepoxy groupsen_US
dc.subjectcovalent bondingen_US
dc.subjectenzyme immobilizationen_US
dc.subjectinvertaseen_US
dc.titleCovalent immobilization of invertase on microporous pHEMA-GMA membraneen_US
dc.typeArticle

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