Investigation of antimicrobial resistance of ES?L, Amp-C, and carbapenemase-producing E. coli strains in retail poultry meats
| dc.contributor.author | Guven, Gulcin | |
| dc.contributor.author | Kizil, Sibel | |
| dc.date.accessioned | 2025-01-21T16:33:21Z | |
| dc.date.available | 2025-01-21T16:33:21Z | |
| dc.date.issued | 2022 | |
| dc.department | Kırıkkale Üniversitesi | |
| dc.description.abstract | Antimicrobial agents are used for suppressing some bacterial infections during the rearing of poultry. In this study; the antibiotic resistance properties of Extended Spectrum (3eta-lactamase (ES(3L), Amp-C, carbapenemase-producing Escherichia coli (E. coli) strains isolated from chicken breast meat were determined by disc diffusion and E-test methods. Additionally, it is aimed to determine antibiotic resistance genes by classical polymerase chain reaction (PCR). In this context, E. coli strains were isolated from 7 of 100 chicken breast meat (fillets) and identified by a rapid identification system (BBL Crystal). Disc diffusion test results of the isolates were evaluated and 100% to tetracycline; ampicillin, ciprofloxacin, cefoxitin, nalidixic acid, oxytetracycline 85.7%; ceftazidime, cefotaxime and meropenem 28.5%; chloramphenicol 71.4%, trimethoprim/sulfamethoxazole 42.8%; 14.2% resistance to gentamicin was observed. Test results of the isolates were evaluated and ES(3L activity was found in 2 isolates in the disc diffusion test. Amp-C and carbapenemase activity was detected in 6 isolates in disc diffusion, in all isolates in E-test; 2 isolates in disc diffusion were detected carbapenemase activity, while carbapenemase activity was not detected in E-test, respectively. Classical PCR was performed after the DNAs were isolated for the detection of antibiotic resistance genes. According to classical PCR results; ES(3L activity was detected in all isolates; carbapenemase activity was positive in only 1 isolate, while Amp-C activity was positive in 4 isolates. CTXM-1 (71%) and SHV1 (71%), OXA-1 (42.8%), CTXM-9 (28.5%), and TEM (14.2%) were detected by classical PCR, respectively. It has been concluded that E. coli isolated from poultry meat collected from the market may pose a risk in terms of ES(3L, Amp-C, and carbapenemase genes, and continuous monitoring is required in this regard. | |
| dc.identifier.doi | 10.55730/1300-0128.4254 | |
| dc.identifier.endpage | 794 | |
| dc.identifier.issn | 1300-0128 | |
| dc.identifier.issue | 6 | |
| dc.identifier.scopus | 2-s2.0-85148726407 | |
| dc.identifier.scopusquality | Q3 | |
| dc.identifier.startpage | 788 | |
| dc.identifier.trdizinid | 1147561 | |
| dc.identifier.uri | https://doi.org/10.55730/1300-0128.4254 | |
| dc.identifier.uri | https://search.trdizin.gov.tr/tr/yayin/detay1147561 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12587/23786 | |
| dc.identifier.volume | 46 | |
| dc.identifier.wos | WOS:000899553100002 | |
| dc.identifier.wosquality | Q4 | |
| dc.indekslendigikaynak | Web of Science | |
| dc.indekslendigikaynak | Scopus | |
| dc.indekslendigikaynak | TR-Dizin | |
| dc.language.iso | en | |
| dc.publisher | Tubitak Scientific & Technological Research Council Turkey | |
| dc.relation.ispartof | Turkish Journal of Veterinary & Animal Sciences | |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.snmz | KA_20241229 | |
| dc.subject | Antimicrobial resistance; Amp-C; carbapenemase; E; coli; ES(3L | |
| dc.title | Investigation of antimicrobial resistance of ES?L, Amp-C, and carbapenemase-producing E. coli strains in retail poultry meats | |
| dc.type | Article |
