A comprehensive study of Helicobacter pylori infection: molecular analysis, antibacterial susceptibility, and histopathological examination

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dc.authoridKarakaya, Emre/0000-0003-2390-6190
dc.contributor.authorBuyuk, Fatih
dc.contributor.authorKarakaya, Emre
dc.contributor.authorAkar, Mustafa
dc.contributor.authorKayman, Tuba
dc.contributor.authorTarhane, Serdal
dc.contributor.authorOzcan, Hacer Ece
dc.contributor.authorCelebi, Ozgur
dc.date.accessioned2025-01-21T16:34:57Z
dc.date.available2025-01-21T16:34:57Z
dc.date.issued2023
dc.departmentKırıkkale Üniversitesi
dc.description.abstractHelicobacter pylori is a pathogen associated with gastroduodenal diseases. This study aimed; (i) to investigate H. pylori presence by invasive tests in adult dyspeptic patients, (ii) to determine antibiotic susceptibility and genotypic characteristics of the H. pylori isolates, and (iii) to investigate the relationship between the H. pylori genotypes and the histopathological findings. In this cross-sectional study, gastric biopsy samples from 208 adult dyspeptic patients were used for culture, tissue Polymerase Chain Reaction (PCR), and histopathological analysis. Antibiotic susceptibility of the H. pylori isolates was analyzed by gradient method. Analysis of the virulence genes was performed by monoplex PCR. Genetic profiles (from A to H) were created based on the virulence genes presence. Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR) was used for the genotyping of the H. pylori isolates. The mean age of the patients was 46 (& PLUSMN; 15) years and 128 (61.5%) of them were female. H. pylori positivity was detected by culture, tissue PCR and histopathological examination in 59 (28.4%), 114 (54.8%) and 81 (38.9%) patients, respectively. The overall prevalence of H. pylori was found to be 63% (131/208). All H. pylori isolates were susceptible to tetracycline and amoxicillin. The resistance rates for metronidazole, clarithromycin, levofloxacin, and rifampicin were 67.2%, 27.9%, 34.4% and 13.11%, respectively. Multi drug resistance (MDR) was detected at the rate of 45.9% (28/61). While the most common virulence gene was cagA (93.44%), the least common was vacAm1 (23%). The predominant genetic profile was profile A (47.5%). ERIC-PCR results revealed a total of 26 different patterns. A high prevalence of H. pylori was detected in adult dyspeptic patients as in developing countries. It was observed significant genotypic heterogeneity and virulence gene diversity within the isolates. A considerable resistance rate detected against antibiotics such as clarithromycin, metronidazole, and levofloxacin, which are frequently used in the eradication of H. pylori, should be taken into consideration when creating regional empirical treatment regimens.
dc.description.sponsorshipKafkas University, Turkiye [2021-TS-11]
dc.description.sponsorshipThe study was supported by research grant funding from the Kafkas University, Turkiye (Grant/Award Number: 2021-TS-11).
dc.identifier.doi10.1007/s10482-023-01868-3
dc.identifier.endpage1273
dc.identifier.issn0003-6072
dc.identifier.issn1572-9699
dc.identifier.issue12
dc.identifier.pmid37603113
dc.identifier.scopus2-s2.0-85168494356
dc.identifier.scopusqualityQ2
dc.identifier.startpage1261
dc.identifier.urihttps://doi.org/10.1007/s10482-023-01868-3
dc.identifier.urihttps://hdl.handle.net/20.500.12587/24042
dc.identifier.volume116
dc.identifier.wosWOS:001052026400001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofAntonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectAntibiotic resistance; Dyspepsia; Gastritis; Genotyping; Helicobacter pylori; Virulence gene
dc.titleA comprehensive study of Helicobacter pylori infection: molecular analysis, antibacterial susceptibility, and histopathological examination
dc.typeArticle

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