Platelet-rich plasma decreases fibroblastic activity and woven bone formation with no significant immunohistochemical effect on long-bone healing: An experimental animal study with radiological outcomes

Basit Öğe Kaydı
dc.contributor.authorCanbeyli, Ibrahim Deniz
dc.contributor.authorAkgun, Rahmi Can
dc.contributor.authorSahin, Orcun
dc.contributor.authorTerzi, Aysen
dc.contributor.authorTuncay, Ismail Cengiz
dc.date.accessioned2020-06-25T18:29:22Z
dc.date.available2020-06-25T18:29:22Z
dc.date.issued2018
dc.departmentKırıkkale Üniversitesi
dc.description.abstractPurpose: This study aimed to analyze the immunohistochemical effect of platelet-rich plasma (PRP) on healing of long-bone fractures in terms of bone morphogenetic protein-2 (BMP-2), vascular endothelial growth factor (VEGF), the Ki-67 proliferation index, and radiological and histological analyses. Methods: Sixteen adult rabbits, whose right femoral diaphysis was fractured and fixed with Kirschner wires, were randomly divided into two groups, control and PRP (groups A and B, respectively). PRP was given to group B at 1 week postoperatively, and all animals were euthanized after 12 weeks. Radiographic evaluations were performed periodically. Cortical callus formation, chondroid and woven bone area percentages, osteoblastic and fibroblastic activities, and mature bone formation were examined. The depths of BMP-2 and VEGF staining were measured. The Ki-67 proliferation index was also calculated. Results: The mean radiological union score of group B was significantly higher than that of group A. There were also statistically significant differences between groups A and B in terms of cortical callus formation, woven bone area percentage, fibroblast proliferation, and mature bone formation. Group B had significantly more cortical callus and mature bone formation with less woven bone and fibroblast proliferation. Immunohistochemical analysis revealed no statistically significant difference between the groups in terms of BMP-2 and VEGF staining and the Ki-67 index. Conclusions: PRP had no effect on BMP-2 or VEGF levels with no increase in the Ki-67 proliferation index, although its application had a positive effect on bone healing by increasing callus and mature bone formation with decreased woven bone and fibroblast proliferation.en_US
dc.description.sponsorshipBaskent University Scientific Research Councilen_US
dc.description.sponsorshipThe author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by Baskent University Scientific Research Council.en_US
dc.identifier.citationCanbeyli İD, Akgun RC, Sahin O, Terzi A, Tuncay İC. Platelet-rich plasma decreases fibroblastic activity and woven bone formation with no significant immunohistochemical effect on long-bone healing: An experimental animal study with radiological outcomes. Journal of Orthopaedic Surgery. 2018;26(3).en_US
dc.identifier.doi10.1177/2309499018802491
dc.identifier.issn2309-4990
dc.identifier.issue3en_US
dc.identifier.pmid30295139
dc.identifier.scopus2-s2.0-85054468506
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1177/2309499018802491
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7272
dc.identifier.volume26en_US
dc.identifier.wosWOS:000446696200001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSage Publications Incen_US
dc.relation.ispartofJournal Of Orthopaedic Surgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectfracture healingen_US
dc.subjectimmunohistopathologyen_US
dc.subjectlong-bone fractureen_US
dc.subjectplatelet-rich plasmaen_US
dc.titlePlatelet-rich plasma decreases fibroblastic activity and woven bone formation with no significant immunohistochemical effect on long-bone healing: An experimental animal study with radiological outcomesen_US
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Platelet-rich plasma decreases fibroblastic activity and woven bone formation with no significant immunohistochemical effect on long-bone healing An experimental animal study with radiological outcomes.pdf
Boyut:
1.63 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text
İndir

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu