Procion Green H-4G immobilized on a new IPN hydrogel membrane composed of poly(2-hydroxyethylmethacrylate)/chitosan: preparation and its application to the adsorption of lysozyme

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dc.contributor.authorBayramoğlu, G.
dc.contributor.authorArica, M.Y.
dc.date.accessioned2020-06-25T17:35:06Z
dc.date.available2020-06-25T17:35:06Z
dc.date.issued2002
dc.departmentKırıkkale Üniversitesi
dc.description.abstractThe adsorption of lysozyme has been investigated on the Procion Green H-4G immobilized novel poly(2-hydroxyethyl methacrylate)/chitosan) (pHEMA/chitosan; IPN) porous hydrogel membrane. The IPN membranes were prepared by UV initiated photo-polymerization using 2-HEMA and chitosan. The IPN membrane reached an equilibrium swelling within about 60 min. The water content of the dye immobilized membrane was 50 +/- 2% and the amount of immobilized Procion Green H-4G on the membrane was 0.678 mumol ml(-1). The rates of adsorption of lysozyme on pHEMA/chitosan and pHEMA/chitosan-dye immobilized membranes were measured in a stirred cell, The adsorption capacities of these membranes were determined by changing pH and the concentration of lysozyme in the adsorption medium. The adsorption phenomena appeared to follow a typical Langmuir isotherm. Lysozyme adsorption capacity of the pHEMA/chitosan and pHEMA/chitosan-dye immobilized membranes were 0.18 and 14.06 mg ml(-1), respectively. The maximum lysozyme adsorption capacity (q(m)) of the pHEMA-chitosan-dye immobilized membranes was 20.28 mg ml(-1) and the dissociation constant (K-d) value was found to be 1.01 mg ml(-1) lysozyme. The experimental data were also analysed using first order Van't Hoff equation for lysozyme-adsorbent interactions, the change in entropy and Gibbs energy of binding were determined at different temperatures and the enthalpy of the system was calculated as 2.5 kcal mol(-1). All of the adsorbed lysozyme were desorbed in 60 min in the desorption medium containing 1.0 M KSNC at pH 8.0. (C) 2002 Elsevier Science B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/S0927-7757(01)01055-X
dc.identifier.endpage52en_US
dc.identifier.issn0927-7757
dc.identifier.issn1873-4359
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-0037177117
dc.identifier.scopusqualityQ1
dc.identifier.startpage41en_US
dc.identifier.urihttps://doi.org/10.1016/S0927-7757(01)01055-X
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3019
dc.identifier.volume202en_US
dc.identifier.wosWOS:000175154200005
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Bven_US
dc.relation.ispartofColloids And Surfaces A-Physicochemical And Engineering Aspects
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectinterpenetrating networksen_US
dc.subjectIPNen_US
dc.subjectpHEMAen_US
dc.subjectchitosanen_US
dc.subjectProcion Green H-4Gen_US
dc.subjection exchange membraneen_US
dc.subjectlysozymeen_US
dc.subjectadsorptionen_US
dc.titleProcion Green H-4G immobilized on a new IPN hydrogel membrane composed of poly(2-hydroxyethylmethacrylate)/chitosan: preparation and its application to the adsorption of lysozymeen_US
dc.typeArticle

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