Delivery of siRNA using hyaluronic acid-guided nanoparticles for downregulation of CXCR4

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dc.authoridBUDAK YILDIRAN, Fatma Azize/0000-0001-7031-6834
dc.contributor.authorTunali, Beste Cagdas
dc.contributor.authorCelik, Eda
dc.contributor.authorYildiran, Fatma Azize Budak
dc.contributor.authorTurk, Mustafa
dc.date.accessioned2025-01-21T16:37:17Z
dc.date.available2025-01-21T16:37:17Z
dc.date.issued2023
dc.departmentKırıkkale Üniversitesi
dc.description.abstractIn this study, effective transport of small interfering RNAs (siRNAs) via hyaluronic acid (HA) receptor was carried out with biodegradable HA and low-molecular weight polyethyleneimine (PEI)-based transport systems. Gold nanoparticles (AuNPs) capable of giving photothermal response, and their conjugates with PEI and HA, were also added to the structure. Thus, a combination of gene silencing, photothermal therapy and chemotherapy, has been accomplished. The synthesized transport systems ranged in size, between 25 and 690 nm. When the particles were applied at a concentration of 100 mu g mL(-1) (except AuPEI NPs) in vitro, cell viability was above 50%. Applying radiation after the conjugate/siRNA complex (especially those containing AuNP) treatment, increased the cytotoxic effect (decrease in cell viability of 37%, 54%, 13%, and 15% for AuNP, AuPEI NP, AuPEI-HA, and AuPEI-HA-DOX, respectively) on the MDA-MB-231 cell line. CXCR4 gene silencing via the synthesized complexes, especially AuPEI-HA-DOX/siRNA was more efficient in MDA-MB-231 cells (25-fold decrease in gene expression) than in CAPAN-1 cells. All these results demonstrated that the synthesized PEI-HA and AuPEI-HA-DOX conjugates can be used as siRNA carriers that are particularly effective, especially in the treatment of breast cancer.
dc.description.sponsorship[TR71/13/GPD/01]
dc.description.sponsorshipACKNOWLEDGMENTS The infrastructure used in the experiments by Ahiler Development Agency (AHIKA) Grant TR71/13/GPD/01 to M.T. is gratefully acknowledged. This study was the outcome of the PhD thesis by B.C.T.
dc.identifier.doi10.1002/bip.23535
dc.identifier.issn0006-3525
dc.identifier.issn1097-0282
dc.identifier.issue4
dc.identifier.pmid36972328
dc.identifier.scopus2-s2.0-85150960486
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1002/bip.23535
dc.identifier.urihttps://hdl.handle.net/20.500.12587/24447
dc.identifier.volume114
dc.identifier.wosWOS:000956945600001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofBiopolymers
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectCXCR4; gene therapy; gold nanoparticle; hyaluronic acid; Polyethylenimine; siRNA
dc.titleDelivery of siRNA using hyaluronic acid-guided nanoparticles for downregulation of CXCR4
dc.typeArticle

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