Apoptotic and necrotic effects of plant extracts belonging to the genus Alchemilla L. species on HeLa cells in vitro

Basit Öğe Kaydı
dc.contributor.authorTurk, Mustafa
dc.contributor.authorKaya, Bulent
dc.contributor.authorMenemen, Yusuf
dc.contributor.authorOguztuzun, Serpil
dc.date.accessioned2020-06-25T17:51:38Z
dc.date.available2020-06-25T17:51:38Z
dc.date.issued2011
dc.departmentKırıkkale Üniversitesi
dc.description.abstractApoptotic and necrotic effects of plant extracts belonging to the genus Alchemilla on HeLa cells were investigated. The ratio of viable cells was determined by cell counter. Double staining method for the determination of apoptotic and necrotic index was carried out. The lowest apoptotic effect was found in Alchemilla oriturcica with the ratio of around 14% and the highest apoptotic effect found in Alchemilla trabzonica was 24%. Apoptotic effect was additionally determined by caspase-3 immunostaining. Increasing the concentrations of the extracts, especially in the level of 150 to 200 mu g/mL caused the increase of necrotic effect on cancer cells. Particularly, the effects of A. oriturcica and A. trabzonica extracts with the density of 200 mu g/mL were very high compared to the other species. The flavonoid components were detected in extracts of the species used for this study. The flavonoids identified are orientin (luteolin-8-C-glucoside), hyperoside (quercetin-3-O-galactoside) and isoquercetin (quercetin-3-glucoside) in Alchemilla erythropoda; rutin (quercetin-3-rutinoside), orientin, vitexin (apigenin-8-C-glucoside), hyperoside in Alchemilla ikizdereensis; rutin, hyperoside, isoquercetin (quercetin-3-glucoside) in A. oriturcica, and hyperoside, isoquercetin and quercitrin (quercetin-3-O-rhamnoside) in A. trabzonica.en_US
dc.description.sponsorshipScience Research Foundation of Kirikkale University of TurkeyKirikkale University [2007/44]en_US
dc.description.sponsorshipThis work was supported by Science Research Foundation of Kirikkale University of Turkey (project number: 2007/44).en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.endpage4571en_US
dc.identifier.issn1996-0875
dc.identifier.issue18en_US
dc.identifier.scopus2-s2.0-81555216099
dc.identifier.scopusqualityN/A
dc.identifier.startpage4566en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12587/4940
dc.identifier.volume5en_US
dc.identifier.wosWOS:000299778500027
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherAcademic Journalsen_US
dc.relation.ispartofJournal Of Medicinal Plants Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlchemillaen_US
dc.subjectextracten_US
dc.subjectHeLa cellen_US
dc.subjectcytotoxicityen_US
dc.subjectapoptosisen_US
dc.subjectnecrosisen_US
dc.titleApoptotic and necrotic effects of plant extracts belonging to the genus Alchemilla L. species on HeLa cells in vitroen_US
dc.typeArticle

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