Light and electron microscope examination of the effects of methotrexate on the endosalpinx

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dc.contributor.authorBayram, Merih
dc.contributor.authorÖzoğul, Candan
dc.contributor.authorDursun, Ayşe
dc.contributor.authorErcan, Z. Sevim
dc.contributor.authorIşık, İpek
dc.contributor.authorDileköz, Ergin
dc.date.accessioned2020-06-25T17:40:39Z
dc.date.available2020-06-25T17:40:39Z
dc.date.issued2005
dc.departmentKırıkkale Üniversitesi
dc.description.abstractObjective: To examine the effects of increasing doses of methotrexate (Mtx) on the fallopian tubes. Study design: The study was carried out on 24 female rats (Albino Wistar type, 250-300 g). The rats were randomly divided into four groups of six. Different doses of Mtx were given to the rats by i.p. injection: 1 mg/kg to those in group 1, 5 mg/kg in group 2 and 10 mg/kg in group 3. Rats in group 4 received injections of physiological serum only and were treated as the control group. Ten days after the injection, the fallopian tubes of the rats were removed for examination separately by light and electron microscopy (EM) for comparison. Results: Light microscopy showed that in group I the surface epithelial cells were normal and the lamina propria was infiltrated by numerous inflammatory cells with a prevalence of polymorphonuclear leucocytes. Findings in groups 2 and 3 were similar: the lamina propria was infiltrated with granulocytes in one specimen from each of the two groups, and granulocytes were also observed among epithelial cells. In the control group all surface structures were found to be in a normal condition. Electron microscopy showed cilial loss in the epithelial cells and central crystolysis in mitochondria in all group I specimens. Findings in groups 2 and 3 were similar. The cytoplasm of the epithelial cells seemed to be dense, there was prominent crystolysis (crystalloid formation) in the mitochondria, and vacuolisation (vacuole formation) in the cytoplasm seemed to be augmented. Cilial loss was prominent, and the basal membrane was irregular. Epithelial cell nuclei were in disorder. Lipid granules were observed extensively in epithelial cells. Eosinophils seemed to be dominant in connective tissues below the epithelium. In all control group specimens the epithelium seemed to be normal with all organelles in place; the condition of intercellular junctions, ciliated epithelium and all mitochondria also seemed to be normal, and the basal membrane was observed to be in order. Conclusion: In view of these findings, we conclude that the ultrastructural derangements resulting from administration of Mtx in doses in excess of I mg/kg can cause a reduction in the surface epithelium's ability to make rhythmic lashing movements and can impair the patency of the fallopian tubes. All these disturbances could be involved to some degree in the causation of infertility and recurrent ectopic pregnancy. Therefore, the dosage of Mtx should be limited to use of the lowest effective dose to avoid these adverse effects. (c) 2004 Elsevier Ireland Ltd. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.ejogrb.2004.08.014
dc.identifier.endpage103en_US
dc.identifier.issn0301-2115
dc.identifier.issn1872-7654
dc.identifier.issue1en_US
dc.identifier.pmid15866094
dc.identifier.scopus2-s2.0-18144401261
dc.identifier.scopusqualityQ2
dc.identifier.startpage96en_US
dc.identifier.urihttps://doi.org/10.1016/j.ejogrb.2004.08.014
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3511
dc.identifier.volume120en_US
dc.identifier.wosWOS:000229276900016
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bven_US
dc.relation.ispartofEuropean Journal Of Obstetrics & Gynecology And Reproductive Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectmethotrexateen_US
dc.subjectendosalpinxen_US
dc.subjectfallopian tubes (tubae uterinae)en_US
dc.subjectlight microscopeen_US
dc.subjectelectron microscopeen_US
dc.titleLight and electron microscope examination of the effects of methotrexate on the endosalpinxen_US
dc.typeArticle

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