Separation of human-immunoglobulin-G from human plasma with L-histidine immobilized pseudo-specific bioaffinity adsorbents

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dc.contributor.authorÖzkara, S.
dc.contributor.authorYavuz, H.
dc.contributor.authorPatir, S.
dc.contributor.authorArica, M.Y.
dc.contributor.authorDenizli, A.
dc.date.accessioned2020-06-25T17:35:12Z
dc.date.available2020-06-25T17:35:12Z
dc.date.issued2002
dc.departmentKırıkkale Üniversitesi
dc.description.abstractThe pseudo-biospecific affinity ligand L-histidine immobilized poly(2-hydroxyethylmethacrylate) (PHEMA) in spherical form (100-150 mum in diameter) was used for the affinity chromatographic separation of human-immunoglobulin-G (HIgG) from aqueous solutions and human plasma. The PHEMA adsorbents were prepared by a radical suspension polymerization technique. Reactive aminoacid-ligand L-histidine was then immobilized by covalent binding onto these adsorbents. Elemental analysis of immobilized L-histidine for nitrogen was estimated as 62.3 mg L-histidine/g of PHEMA. The maximum HIgG adsorption on the L-histidine immobilized PHEMA adsorbents was observed at pH 7.4. The non-specific HIaG adsorption onto the plain PHEMA adsorbents was very low- (about 0.167 mg/g). Higher adsorption values (up to 3.5 mg/g) were obtained when the L-histidine immobilized PHEMA adsorbents were used from aqueous solutions. Much higher amounts of HIgG were adsorbed from human plasma (up to 44.8 mg/g). Adsorption capacities of other blood proteins were obtained as 2.2 mg/g for fibrinogen and 2.8 mg/g for albumin. The total protein adsorption was determined as 52.1 mg/g. The affinity microbeads allowed the one-step separation of HIgG from human plasma. The HIgG molecules could be repeatedly adsorbed and desorbed with these L-histidine-immobilized PHEMA adsorbents without noticeable loss in their HIgG adsorption capacity.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1081/SS-120001456
dc.identifier.endpage731en_US
dc.identifier.issn0149-6395
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-0036195451
dc.identifier.scopusqualityQ2
dc.identifier.startpage717en_US
dc.identifier.urihttps://doi.org/10.1081/SS-120001456
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3052
dc.identifier.volume37en_US
dc.identifier.wosWOS:000174241300013
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherMarcel Dekker Incen_US
dc.relation.ispartofSeparation Science And Technology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleSeparation of human-immunoglobulin-G from human plasma with L-histidine immobilized pseudo-specific bioaffinity adsorbentsen_US
dc.typeArticle

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