Studies on the activity and stability of immobilized horseradish peroxidase on poly(ethylene terephthalate) grafted acrylamide fiber
Yükleniyor...
Tarih
2009
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Having been activated with glutaraldehyde, modified poly(ethylene terephthalate) grafted acrylamide fiber was used for the immobilization of horseradish peroxidase (HRP). Both the free HRP and the immobilized HRP were characterized by determining the activity profile as a function of pH, temperature, thermal stability, effect of organic solvent and storage stability. The optimum pH values of the enzyme activity were found as 8 and 7 for the free HRP and the immobilized HRP respectively. The temperature profile of the free HRP and the immobilized HRP revealed a similar behaviour, although the immobilized HRP exhibited higher relative activity in the range from 50 to 60 A degrees C. The immobilized HRP showed higher storage stability than the free HRP.
Açıklama
Temocin, Zulfikar/0000-0001-7151-9772
Anahtar Kelimeler
Horseradish peroxidase, Immobilization, Poly(ethylene terephthalate), Fibers, Graft copolymers
Kaynak
Bioprocess And Biosystems Engineering
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
32
Sayı
4
Künye
closedAccess
