Immobilization of laccase in κ-carrageenan based semi-interpenetrating polymer networks

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dc.contributor.authorMakas, Y. Gizem
dc.contributor.authorKalkan, N. Ayça
dc.contributor.authorAksoy, Serpil
dc.contributor.authorAltınok, Haydar
dc.contributor.authorHasırcı, Nesrin
dc.date.accessioned2020-06-25T17:49:20Z
dc.date.available2020-06-25T17:49:20Z
dc.date.issued2010
dc.departmentKırıkkale Üniversitesi
dc.description.abstractLaccase enzyme (L) was immobilized by entrapment into semi-interpenetrating polymer networks (semi-IPNs) prepared from kappa-carrageenan with either poly(acrylamide-acrylic acid) [P(AAm-AA)/kappa-car] or poly(acrylamide-itaconic acid) [P(AAm-IA)/kappa-car]. For both systems, immobilized enzymes achieved the same optimum values observed for free enzyme (pH = 5.0 and T = 40 degrees C), except for P(AAm-IA)/kappa-car system there was a shift to 5.5 in optimum pH value. At the end of 42 days of storage immobilized enzymes retained more than 80% of their original activities while the retained activities of both systems after 10 uses in batch type application were found to be higher than 50%. K-m values were calculated as 2.52 x 10(-2) mM and 1.08 x 10(-2) mM and V-max values were found as 6.8 x 10(-3) mM min(-1) and 4.4 x 10(-3) mM min(-1), for P(AAm-AA)/kappa-car-L and P(AAm-IA)/kappa-car-L, respectively. When methyl orange containing solutions (10 mg/L) were treated with the immobilized laccases (68.2 U), enzymatic decolorization of methyl orange in 6 h was achieved to the level of 35% for both systems. Supplementing the reaction medium with ABTS as the redox mediator increased this value to about 70%. These initial results show that, laccase containing semi-IPNs can find some applications in decolorization of the industrial wastes. (C) 2010 Elsevier B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.jbiotec.2010.05.011
dc.identifier.endpage220en_US
dc.identifier.issn0168-1656
dc.identifier.issn1873-4863
dc.identifier.issue4en_US
dc.identifier.pmid20540972
dc.identifier.scopus2-s2.0-77955056144
dc.identifier.scopusqualityQ1
dc.identifier.startpage216en_US
dc.identifier.urihttps://doi.org/10.1016/j.jbiotec.2010.05.011
dc.identifier.urihttps://hdl.handle.net/20.500.12587/4694
dc.identifier.volume148en_US
dc.identifier.wosWOS:000280972500007
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bven_US
dc.relation.ispartofJournal Of Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSemi-IPNsen_US
dc.subjectImmobilized laccaseen_US
dc.subjectkappa-Carrageenanen_US
dc.subjectDecolorizationen_US
dc.subjectMethyl orangeen_US
dc.titleImmobilization of laccase in κ-carrageenan based semi-interpenetrating polymer networksen_US
dc.typeArticle

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