Affinity microspheres and their application to lysozyme adsorption: Cibacron Blue F3GA and Cu(II) with poly(HEMA-EGDMA)

Basit Öğe Kaydı
dc.contributor.authorDenizli, F
dc.contributor.authorDenizli, A
dc.contributor.authorArica, MY
dc.date.accessioned2020-06-25T17:34:39Z
dc.date.available2020-06-25T17:34:39Z
dc.date.issued1999
dc.departmentKırıkkale Üniversitesi
dc.description.abstractLysozyme adsorption onto Cibacron Blue F3GA attached and Cu(II) incorporated poly(2-hydroxyethyl methacrylate-ethylene glycol dimethacrylate) [poly(HEMA-EGDMA)] microspheres was investigated. The microspheres were prepared by suspension polymerization. Various amounts of Cibacron Blue F3GA were attached covalently onto the microspheres by changing the initial concentration of dye in the reaction medium. The microspheres with a swelling ratio of 65%, and carrying different amounts of dye (between 1.4 and 22.5 mu mol/g(-1)) were used in the lysozyme adsorption studies. Lysozyme adsorption on these microspheres from aqueous solutions containing different amounts of lysozyme at different pH values was investigated in batch reactors. The lysozyme adsorption capacity of the dye-metal chelated microspheres (238.2 mg g(-1)) was greater than that of the dye-attached microspheres (175.1 mg g(-1)). The maximum lyzozyme adsorption capacities (q(m)) and the dissociation constant (k(d)) values were found to be 204.9 mg g(-1) and 0.0715 mg ml(-1) with dye-attached and 270.7 mg g(-1) and 0.0583 mg ml(-1) with dye-metal chelated microspheres, respectively. More than 90% of the adsorbed lysozyme were desorbed in 60 min in the desorption medium containing 0.5 M KSCN at pH 8.0 or 25 mM. EDTA at pH 4.9. (C) 1999 Society of Chemical Industry.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1002/(SICI)1097-0126(199905)48:5<360
dc.identifier.endpage366en_US
dc.identifier.issn0959-8103
dc.identifier.issn1097-0126
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-0032809828
dc.identifier.scopusqualityQ1
dc.identifier.startpage360en_US
dc.identifier.urihttps://doi.org/10.1002/(SICI)1097-0126(199905)48:5<360
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2824
dc.identifier.volume48en_US
dc.identifier.wosWOS:000080289900004
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWileyen_US
dc.relation.ispartofPolymer International
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectlysozymeen_US
dc.subjectpHEMA EGDMA microspheresen_US
dc.subjectCibacron Blue F3GAen_US
dc.subjectadsorptionen_US
dc.subjectmetal chelatesen_US
dc.subjectaffinityen_US
dc.titleAffinity microspheres and their application to lysozyme adsorption: Cibacron Blue F3GA and Cu(II) with poly(HEMA-EGDMA)en_US
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Affinity microspheres and their application to lysozyme adsorption Cibacron Blue F3GA and Cu(II) with poly(HEMA-EGDMA).pdf
Boyut:
138.72 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text
İndir

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu