Immobilization of catalase via adsorption onto L-histidine grafted functional pHEMA based membrane

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dc.contributor.authorAkgöl, S.
dc.contributor.authorKacar, Y.
dc.contributor.authorÖzkara, S.
dc.contributor.authorYavuz, H.
dc.contributor.authorDenizli, A.
dc.contributor.authorArica, M.Y.
dc.date.accessioned2020-06-25T17:34:47Z
dc.date.available2020-06-25T17:34:47Z
dc.date.issued2001
dc.departmentKırıkkale Üniversitesi
dc.descriptionKacar, Yasemin/0000-0002-8682-9228; Akgol, Sinan/0000-0002-8528-1854; AKGOL, Sinan/0000-0003-2836-7181
dc.description.abstractPoly(2-hydroxyethylmethacrylate) (pHEMA) based flat sheet membrane was prepared by UV-initiated photopolymerization technique. The membrane was then grafted with L-histidine. Catalase immobilization onto the membrane from aqueous solutions containing different amounts of catalase at different pH was investigated in a batch system. The maximum catalase immobilization capacity of the pHEMA-histidine membrane was 86 mug cm(-2). The activity yield was decreased with the increase of the enzyme loading. It was observed that there was a significant change between V-max value of the free catalase and V-max value of the adsorbed catalase on the pHEMA-histidine membrane. The K-m value of the immobilized enzyme was higher 1.5 times than that of the free enzyme. Optimum operational temperature was 5 degreesC higher than that of the free enzyme and was significantly broader. It was observed that enzyme could be repeatedly adsorbed and desorbed without loss of adsorption capacity or enzyme activity. (C) 2001 Elsevier Science B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/S1381-1177(01)00029-7
dc.identifier.endpage206en_US
dc.identifier.issn1381-1177
dc.identifier.issue4-6en_US
dc.identifier.scopus2-s2.0-0035501579
dc.identifier.scopusqualityN/A
dc.identifier.startpage197en_US
dc.identifier.urihttps://doi.org/10.1016/S1381-1177(01)00029-7
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2890
dc.identifier.volume15en_US
dc.identifier.wosWOS:000171658200011
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Bven_US
dc.relation.ispartofJournal Of Molecular Catalysis B-Enzymatic
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcatalaseen_US
dc.subjectimmobilizationen_US
dc.subjectadsorptionen_US
dc.subjectL-histidineen_US
dc.subjectpHEMA membraneen_US
dc.titleImmobilization of catalase via adsorption onto L-histidine grafted functional pHEMA based membraneen_US
dc.typeArticle

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