Immobilization of β-galactosidase onto magnetic poly(GMA–MMA) beads for hydrolysis of lactose in bed reactor

Basit Öğe Kaydı
dc.contributor.authorBayramogğu, Gülay
dc.contributor.authorTunali, Yagmur
dc.contributor.authorArica, M. Yakup
dc.date.accessioned2020-06-25T17:43:43Z
dc.date.available2020-06-25T17:43:43Z
dc.date.issued2007
dc.description.abstractIn the present study, novel magnetic beads were prepared from glycidylmethacrylate and methylmethacrylate via suspension polymerization in the presence of a cross-linker (i.e. ethylenedimethylmethacrylate). The magnetic poly(GMA-MMA) beads were characterized with scanning electron microscope, FT-IR and ESR spectrophotometers. The reactive character of the epoxy groups allowed the attachment of the amino groups. The aminated magnetic beads were used for the covalent immobilization of beta-galactosidase via glutaric dialdehyde activation. The maximum amount of immobilized beta-galactosidase on the magnetic poly(GMA-MMA) beads was 9.87 mg/g support. The values of Michaelis constants K-m for immobilized beta-galactosidase was significant larger, indicating decreased affinity by the enzyme for its substrate, whereas V-max values were smaller for the immobilized beta P-galactosidase. However, the beta-galactosidase immobilized on the magnetic poly(GMA-MMA) beads resulted in an increase in enzyme stability with time. Optimum operational temperature for immobilized enzyme was 5 degrees C higher than that of the free enzyme and was significantly broader. Finally, a bed reactor with P-galactosidase immobilized was used for hydrolysis of lactose. The enzyme reactor operated continuously at 35 degrees C for 60 h and the immobilized enzyme lost about 12% of its initial activity after this period. (C) 2006 Elsevier B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.catcom.2006.10.029
dc.identifier.endpage1101en_US
dc.identifier.issn1566-7367
dc.identifier.issn1873-3905
dc.identifier.issue7en_US
dc.identifier.scopus2-s2.0-34248232512
dc.identifier.scopusqualityQ2
dc.identifier.startpage1094en_US
dc.identifier.urihttps://doi.org10.1016/j.catcom.2006.10.029
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3867
dc.identifier.volume8en_US
dc.identifier.wosWOS:000247133800024
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevieren_US
dc.relation.ispartofCatalysis Communications
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectmagnetic beadsen_US
dc.subjectenzyme immobilizationen_US
dc.subjectbeta-galactosidaseen_US
dc.subjectkinetic parametersen_US
dc.subjectenzyme reactoren_US
dc.titleImmobilization of β-galactosidase onto magnetic poly(GMA–MMA) beads for hydrolysis of lactose in bed reactoren_US
dc.typeArticle

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