Effect of spacer-arm and Cu(II) ions on performance of L-histidine immobilized on poly(GMA/MMA) beads as an affinity ligand for separation and purification of IgG

Basit Öğe Kaydı
dc.contributor.authorBayramoğlu, Gülay
dc.contributor.authorŞenel, Aysegül Ülkü
dc.contributor.authorArıca, M. Yakup
dc.date.accessioned2020-06-25T17:43:31Z
dc.date.available2020-06-25T17:43:31Z
dc.date.issued2006
dc.description.abstractIn this study, the beads were prepared from glycidiyl methacrylate (GMA) and methyl methacrylate (MMA) via suspension polymerization and, the used beads fractions were between 75 and 150 mu m. The epoxy groups of the beads were converted into amino groups by the reaction of ammonia or 1,6-diaminohexane as a spacer-arm. L-Histidine ligand was immobilized onto both beads. Cu(II) ions were chelated onto spacer-arm attached and L-histidine immobilized beads. The IgG adsorption capacity of the spacer-arm attached and Cu(II) chelated affinity beads led to higher adsorption capacities about 1.64- and 2.94-fold, respectively. The adsorption equilibrium studies showed that the adsorption isotherm of IgG obeyed the Langmuir isotherm model. The experimental data was well described by the second-order equations. Purification data of IgG with spacer-arm attached and Cu(II) ions chelated (i.e. poly(GMA/MMA)-SAH-Cu(II)) beads indicated that 87.5% of IgG was removed from human serum with a purity of 90%. (C) 2005 Elsevier B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.seppur.2005.11.030
dc.identifier.endpage239en_US
dc.identifier.issn1383-5866
dc.identifier.issn1873-3794
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-33747125111
dc.identifier.scopusqualityQ1
dc.identifier.startpage229en_US
dc.identifier.urihttps://doi.org10.1016/j.seppur.2005.11.030
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3762
dc.identifier.volume50en_US
dc.identifier.wosWOS:000238415800011
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevieren_US
dc.relation.ispartofSeparation And Purification Technology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectaffinity beadsen_US
dc.subjecthistidineen_US
dc.subjectIgGen_US
dc.subjectadsorptionen_US
dc.subjectpurificationen_US
dc.subjectthermodynamic parametersen_US
dc.titleEffect of spacer-arm and Cu(II) ions on performance of L-histidine immobilized on poly(GMA/MMA) beads as an affinity ligand for separation and purification of IgGen_US
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Effect of spacer-arm and Cu(II) ions on performance of L-histidine immobilized on poly(GMA MMA) beads as an affinity ligand for separation and purification of IgG.pdf
Boyut:
416.74 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text
İndir

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
Makale Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu