Novel hydrophobic ligand-containing hydrogel membrane matrix: preparation and application to gamma-globulins adsorption

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dc.contributor.authorArica, MY
dc.contributor.authorAkin-Oktem, G
dc.contributor.authorDenizli, A
dc.date.accessioned2020-06-25T17:34:49Z
dc.date.available2020-06-25T17:34:49Z
dc.date.issued2001
dc.departmentKırıkkale Üniversitesi
dc.description.abstractIn this study, phenylalanine as a hydrophobic ligand was covalently attached to the co-monomer methacrylochloride. Then, poly(2-hydroxyethylmethacrylate-co-methacrylamidophenyalanine) [poly(HEMA-MApA)] membranes were prepared by UV-initiated photopolymerization of HEMA and methacrylamidophenyalanine. The gamma -globulins adsorption onto these affinity membranes from aqueous solutions containing different amounts of gamma -globulins at different pH was investigated in a batch system. The gamma -globulins adsorption capacity of the membranes was increased as the ligand density on the membrane surface increase. The non-specific adsorption of the gamma -globulins on the pHEMA membranes was negligible. The adsorption phenomena appeared to follow a typical Langmuir isotherm. The maximum adsorption capacity (q(m)) of the poly(HEMA-MAPA4) membrane for gamma -globulins was 2.37 mg g(-1) dry membrane. The equilibrium constant (k(d)) value was found to be 1.61 x 10(-1) mg ml(-1). More than 87% (up to 100%) of the adsorbed gamma -globulins were desorbed in 120 min in the desorption medium containing 50% ethylene glycol in 1.0 M NaCl. (C) 2001 Elsevier Science B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.endpage283en_US
dc.identifier.issn0927-7765
dc.identifier.issue4en_US
dc.identifier.pmid11397630
dc.identifier.startpage273en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2914
dc.identifier.volume21en_US
dc.identifier.wosWOS:000169665200004
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bven_US
dc.relation.ispartofColloids And Surfaces B-Biointerfaces
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectaffinity membraneen_US
dc.subjecthydrophobic liganden_US
dc.subjectphenylalanineen_US
dc.subjectgamma-globulinsen_US
dc.subjectprotein separationen_US
dc.titleNovel hydrophobic ligand-containing hydrogel membrane matrix: preparation and application to gamma-globulins adsorptionen_US
dc.typeArticle

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