Listeria monocytogenes risk associated with chicken at slaughter and biocontrol with three new bacteriophages

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dc.contributor.authorÇufaoğlu, Gizem
dc.contributor.authorAyaz, Naim Deniz
dc.date.accessioned2020-06-25T18:34:02Z
dc.date.available2020-06-25T18:34:02Z
dc.date.issued2019
dc.departmentKırıkkale Üniversitesi
dc.description.abstractThe aim of this study was to isolate Listeria monocytogenes from chicken neck skins and lytic bacteriophages from poultry slaughterhouse wastewaters, and following the characterization of the isolates, biocontrol of L. monocytogenes was investigated on chicken drumsticks with the isolated phages. L. monocytogenes prevalence was detected 12.3% in the chicken samples and the dominant serotype was determined as 1/2a (92.5%). Expression levels of major virulence genes were revealed by real-time RT-PCR. Ten different DNA profiles were detected by ERIC-PCR fingerprinting. According to the MIC results, LM-P75 was defined as MDR by showing resistance to antibiotics in six different groups. Besides, five lytic listeriophages were isolated from wastewaters and treated with Cla1 and Sac1. Taking EoP, TEM, in vitro, and in vivo analyses results into consideration, three bacteriophages were used for the biocontrol assay. The application of the bacteriophages on drumsticks achieved a reduction up to 3.3 log CFU/ml in L. monocytogenes count in 3 hr of incubation at 4 degrees C. Practical applications Our results showed that in spite of the developments in hygiene practices during slaughtering, chicken meat is still a potential source for L. monocytogenes. On the other hand, the phage cocktail that used in this study can be an effective tool to reduce L. monocytogenes in chicken carcasses at final wash or at cooling step in poultry slaughtering process, as well as in decontamination of chicken meat parts.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [114R104]; Scientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [114R104]en_US
dc.description.sponsorshipThe Scientific and Technological Research Council of Turkey (TUBITAK), Grant/Award Number: 114R104; Scientific and Technological Research Council of Turkey, Grant/Award Number: 114R104en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1111/jfs.12621
dc.identifier.issn0149-6085
dc.identifier.issn1745-4565
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85059664433
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1111/jfs.12621
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7771
dc.identifier.volume39en_US
dc.identifier.wosWOS:000469985500003
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWileyen_US
dc.relation.ispartofJournal Of Food Safety
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleListeria monocytogenes risk associated with chicken at slaughter and biocontrol with three new bacteriophagesen_US
dc.typeArticle

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