Alternative screening method for analyzing the water samples through an electrical microfluidics chip with classical microbiological assay comparison of P. aeruginosa

Basit Öğe Kaydı
dc.contributor.authorBilican, Ismail
dc.contributor.authorBahadir, Tolga
dc.contributor.authorBilgin, Kemal
dc.contributor.authorGuler, Mustafa Tahsin
dc.date.accessioned2021-01-14T18:10:22Z
dc.date.available2021-01-14T18:10:22Z
dc.date.issued2020
dc.departmentKKÜ
dc.descriptionBAHADIR, TOLGA/0000-0001-9647-0338; GULER, MUSTAFA TAHSIN/0000-0002-0478-3183
dc.description.abstractPseudomonas aeruginosa is a pathogenic bacterium in fresh water supplies that creates a risk for public health. Microbiological analysis of drinking water samples is time consuming and requires qualified personnel. Here we offer a screening system for rapid analysis of spring water that has the potential to be turned into a point-of-need system by means of simple mechanism. The test, which takes 1 h to complete, electrically interrogates the particles through a microfluidic chip suspended in the water sample. We tested the platform using water samples with micro beads and water samples spiked with P. aeruginosa at various concentrations. The mono disperse micro beads were used to evaluate the performance of the system. The results were verified by the gold standard membrane filtration method, which yielded a positive test result only for the P. aeruginosa spiked samples. Detection of 0-11 k bacteria in 30 mu L samples was successfully completed in 1 h and compared with a conventional microbiological method. The presented method is a good candidate for a rapid, on-site, screening test that can result in a significant reduction in cost and analysis time compared to microbiological analyses routinely used in practice.en_US
dc.description.sponsorshipAksaray University (BAP Project) [2018-047]en_US
dc.description.sponsorshipThe authors would like to thank Cabir Cagri Gence for his comments on the manuscript. This study was financially supported by Aksaray University (BAP Project: 2018-047).en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.talanta.2020.121293
dc.identifier.issn0039-9140
dc.identifier.issn1873-3573
dc.identifier.pmid32887035
dc.identifier.scopus2-s2.0-85086897307
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2020.121293
dc.identifier.urihttps://hdl.handle.net/20.500.12587/12541
dc.identifier.volume219en_US
dc.identifier.wosWOS:000568750500006
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherELSEVIERen_US
dc.relation.ispartofTALANTA
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMicrofluidic chipen_US
dc.subjectElectrical detectionen_US
dc.subjectP. aeruginosaen_US
dc.subjectBacteriaen_US
dc.subjectWater analysisen_US
dc.titleAlternative screening method for analyzing the water samples through an electrical microfluidics chip with classical microbiological assay comparison of P. aeruginosaen_US
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Alternative screening method for analyzing the water samples through an electrical microfluidics chip with classical microbiological assay comparison of P. aeruginosa.pdf
Boyut:
9 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text
İndir

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu