In vitro and in vivo effects of commercial and environmental microplastics on Unio delicatus

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dc.authoridFilazi, Ayhan/0000-0002-2800-6215
dc.authoridGUNAL, AYSEL CAGLAN/0000-0002-9072-543X
dc.authoridTavsanoglu, Ulku Nihan/0000-0001-8462-415X
dc.contributor.authorYuce, Pinar Arslan
dc.contributor.authorGunal, Aysel Caglan
dc.contributor.authorErkmen, Belda
dc.contributor.authorYurdakok-Dikmen, Begum
dc.contributor.authorCagan, Ali Serhan
dc.contributor.authorCirak, Tamer
dc.contributor.authorKankilic, Gokben Basaran
dc.date.accessioned2025-01-21T16:41:43Z
dc.date.available2025-01-21T16:41:43Z
dc.date.issued2024
dc.departmentKırıkkale Üniversitesi
dc.description.abstractMicroplastics (MPs) are ubiquitous pollutants in freshwater environments. In this study, freshwater mussels, Unio delicatus, were exposed to both environmental MPs (e-MP) and commercial MPs (c-MP) that include green fluorescent MP (gf-MP), polyethylene (c-PE) and polystyrene (c-PS) at environmental concentrations (5 mg/L and 50 mg/L) over duration of 7 and 30 days. According to in vivo experiment results, both e-MPs and c-MPs induced significant changes in the total hemocyte counts of mussels (p < 0.05). Exposure to high concentrations of e-MPs and c-MPs for 7 days led to decreased cellular glutathione levels in the mussels, while exposure to low concentrations of e-MPs and c-PS for 7 days resulted in increased advanced oxidation protein products (AOPP). Mussels exposed to high concentrations of e-MPs for 30 days exhibited decreases in both glutathione levels and AOPP values. Although no damage was observed in tissues other than gills and digestive gland, histopathological alterations were observed in these tissues following exposure to 50 mg/L c-MPs. Additionally, MPs were observed in the intestine tissues. In vitro experiments using the MTT assay showed no significant difference in cell viability between the MP-exposed group and the control group at tested concentrations, with no observed dose-response relationship (p > 0.05). Nevertheless, certain cells exhibited signs of cell death, such as disrupted cellular structures, condensed nuclei, and loss of cellular integrity. These observations were consistent with mechanical compression, indicating that physical contact with MPs may result in cell damage or death. These findings demonstrate that environmentally relevant concentrations of MPs have toxic effects on freshwater mussels and multiple parameters provide valuable insight for the evaluation of health risks of organisms.
dc.description.sponsorshipScientific and Technological Research Council of Turkiye (Tubitak) [119Y031]
dc.description.sponsorshipThis study was supported by The Scientific and Technological Research Council of Turkiye (Tubitak), Project no: 119Y031. We would like to thank Goktug Gul, Donald Romaric Y. Tessi, Eda Akdag, Sami Can Durak, and Gul Turanoglu for laboratory assistance. Special thanks to M. Zeki Yildirim for the identification of the species of freshwater mussels.
dc.identifier.doi10.1007/s10646-024-02807-2
dc.identifier.issn0963-9292
dc.identifier.issn1573-3017
dc.identifier.pmid39387969
dc.identifier.scopus2-s2.0-85206349736
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1007/s10646-024-02807-2
dc.identifier.urihttps://hdl.handle.net/20.500.12587/24935
dc.identifier.wosWOS:001336739000002
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofEcotoxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectFreshwater mussel; Glutathione level; Advanced oxidation protein products; Histopathology; Cytotoxicity
dc.titleIn vitro and in vivo effects of commercial and environmental microplastics on Unio delicatus
dc.typeArticle

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