Protein A-immobilized microporous polyhydroxyethylmethacrylate affinity membranes for selective sorption of human-immunoglobulin-G from human plasma

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dc.contributor.authorDenizli, A
dc.contributor.authorArica, Y
dc.date.accessioned2020-06-25T17:34:45Z
dc.date.available2020-06-25T17:34:45Z
dc.date.issued2000
dc.departmentKırıkkale Üniversitesi
dc.description.abstractMicroporous membranes made of poly(2-hydroxyethylmethacrylate) [poly(HEMA)] carrying protein A were used for selective sorption of human-IgG from human plasma. Poly(HEMA) membranes were prepared by a photo-polymerization technique, and activated by cyanogen bromide (CNBr) in an alkaline medium (pH 11.5). Bioligand protein A was then immobilized by covalent binding onto these CNBr-activated membranes. The amount of immobilized protein A was controlled by changing pH and the initial concentrations of CNBr and protein A. The non-specific adsorption of protein A on the plain poly(HEMA) membranes was 2.9 mu g cm(-2). Maximum protein A immobilization was observed at pH 9.5. Up to 180 mu g cm(-2) was immobilized on the CNBr-activated poly(HEMA) membranes. The maximum adsorption of human-IgG on the protein A-immobilized poly(HEMA) membranes was observed at pH 8.0. The non-specific adsorption of human-IgG onto the plain poly(HEMA) membranes was low (about 4.4 mu g cm(-2)). Higher human-IgG adsorption values (up to 394 mu g cm(-2)) were obtained in which the protein A-immobilized poly(HEMA) membranes were used. Much higher amounts of human-Igg (up to 489 mu g cm(-2)) were adsorbed from human plasma. Up to 91 % of the adsorbed human-IgG was desorbed by using 0.1 M aminoacetic acid as elution agent. The adsorption-desorption cycle was repeated ten times using the same polymeric membranes. There was no remarkable reduction in the adsorption capacity of the protein A-immobilized poly(HEMA) membranes.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1163/156856200743760
dc.identifier.endpage382en_US
dc.identifier.issn0920-5063
dc.identifier.issue4en_US
dc.identifier.pmid10903036
dc.identifier.scopus2-s2.0-0034123607
dc.identifier.scopusqualityQ1
dc.identifier.startpage367en_US
dc.identifier.urihttps://doi.org/10.1163/156856200743760
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2873
dc.identifier.volume11en_US
dc.identifier.wosWOS:000087993600004
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherVsp Bven_US
dc.relation.ispartofJournal Of Biomaterials Science-Polymer Edition
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleProtein A-immobilized microporous polyhydroxyethylmethacrylate affinity membranes for selective sorption of human-immunoglobulin-G from human plasmaen_US
dc.typeArticle

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