Alteration of tumor glucose metabolism after radiotherapy in MCF-7 breast cancer cell lines

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dc.contributor.authorCengiz M.
dc.contributor.authorBoyunağa H.
dc.contributor.authorYildiz F.
dc.contributor.authorUral A.U.
dc.contributor.authorAtahan I.L.
dc.date.accessioned2020-06-25T15:13:47Z
dc.date.available2020-06-25T15:13:47Z
dc.date.issued2006
dc.departmentKırıkkale Üniversitesi
dc.description.abstractCancer cells utilize anaerobic glycolytic way to compensate their faster metabolism when compared to normal cells. The purpose of this study is to investigate the effect of radiation on tumor metabolism. MCF-7 breast cancer cell lines were divided into 4 groups, including 2 control groups and aerobic and anaerobic study groups (were irradiated 600 cGy by Co-60 teletherapy unit), incubated with radiolabelled glucose for 4 hours. One control group was for aerobic, and the other was for anaerobic group after KCN addition. Radiolabelled CO2 produced by the cells was isolated and collected in specially designed simulation vials. In supernatant the measurements of other end-products of carbohydrate catabolism including lactate, pyruvate, acetate were performed on a liquid scintillation analyzer after they were collected via anion-exchange chromatography. Finally glucose in supernatant was measured enzymatically by glucose oxidase method. Glycogen consumption and lactate production were significantly higher in anaerobic and radiation groups (p<0.01). Whereas CO2 production was significantly higher in control group (p<0.01). Taken all results together radiation lead tumor cells more anaerobic glycolysis with high glycogen consumption, high lactate production and low CO2 production. Radiation itself has led tumor cells to produce energy by anaerobic glycolysis, meaning radiation exposed cells become more hypoxic.en_US
dc.identifier.citationCengiz, M., Boyunağa, H., Yıldız, F., Ural, A. U., Atahan, İ. L.(2006). Alteration of tumor glucose metabolism after radiotherapy in MCF-7 breast cancer cell lines. Uluslararası Hematoloji-Onkoloji Dergisi, 16(4), 172 - 177.en_US
dc.identifier.endpage177en_US
dc.identifier.issn1306133X
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-33845924369
dc.identifier.scopusqualityQ4
dc.identifier.startpage172en_US
dc.identifier.trdizinidtrdizinikyoktur
dc.identifier.urihttps://hdl.handle.net/20.500.12587/1928
dc.identifier.volume16en_US
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherUHOD - Uluslararasi Hematoloji Onkoloji Dergisien_US
dc.relation.ispartofUHOD - Uluslararasi Hematoloji-Onkoloji Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectHypoxiaen_US
dc.subjectMCF-7 cell lineen_US
dc.subjectRadiationen_US
dc.subjectTumor metabolismen_US
dc.titleAlteration of tumor glucose metabolism after radiotherapy in MCF-7 breast cancer cell linesen_US
dc.typeArticle

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